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:: Volume 28, Issue 4 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(4): 270-276 Back to browse issues page
The effects of ubiquinone antioxidants on the MCL1 gene in the CA1 pyramidal cells of hippocampus of male Wistar rat following transient reperfusion inclusive ischemia
Maryam Shirvani Shahenayati 1, Maliheh Entezari * 2
1- MSc in Molecular Genetics, Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
2- Department of Biochemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mentezari@iautmu.ac.ir
Abstract:   (302 Views)
Background: The aim of this study was to evaluate effect of ubiquinone on MCL1 gene expression in hippocampal CA1 pyramidal cells following transient global ischemia/reperfusion in male Wistar rat.
Materials and methods: In this study, we randomly selected 24 Wistar rats under 250-300 g weights that divided into 4 groups including group of observance (maintenance in the laboratory standard condition), ischemia to close the carotid artery and ischemia for 20 minutes, experiment to obtain CoQ10 dose and again obtain CoQ10 coenzyme, and group of control to obtain CoQ10 coenzyme solvent and ischemia again to obtain CoQ10 coenzyme solvent. CoQ10 coenzyme was injected via intraperitoneal (IP) method. The separated hippocampus from the brain was studied by expressing the changes of MCL1 gene.
Results: The expression of MCL1 anti-apoptic gene in observance group has been decreased compared to experiment group. There were not significant differences in the expression of MCL1 gene in the ischemia group towards control group. The expression of MCL1 gene have no significant differences in the coenzyme of control group towards observance group.
Conclusion: According to the results, CoQ10 can create the protective effects in the severity of lesions of hippocampus after ischemia and also increases the rate of the expression gene.
 
Keywords: CoQ10, Cerebral ischemia, Oxidative stress, Free radicals, Apoptosis, Hippocampus, Real time PCR.
Full-Text [PDF 268 kb]   (111 Downloads)    
Semi-pilot: Experimental | Subject: Animal Biology
Received: 2018/01/27 | Accepted: 2018/05/16 | Published: 2018/12/23
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Shirvani Shahenayati M, Entezari M. The effects of ubiquinone antioxidants on the MCL1 gene in the CA1 pyramidal cells of hippocampus of male Wistar rat following transient reperfusion inclusive ischemia . MEDICAL SCIENCES. 2018; 28 (4) :270-276
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Volume 28, Issue 4 (winter 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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