[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
About the Journal
Editorial Board
Aims& Scopes
Journal News
Articles archive::
Current Issue
All Issues
For Authors::
Call for Papers
Submission Instruction
Submission Form
For Reviewers::
Reviewers Section
Registration::
Registration Information
Registration Form
Contact us::
Contact Information
Contact us
Site Facilities::
Site map
Search contents
FAQ
Top 10 contents
Inform to friends
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 28, Issue 4 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(4): 270-276 Back to browse issues page
The effects of ubiquinone antioxidants on the MCL1 gene in the CA1 pyramidal cells of hippocampus of male Wistar rat following transient reperfusion inclusive ischemia
Maryam Shirvani Shahenayati1 , Maliheh Entezari 2
1- MSc in Molecular Genetics, Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
2- Department of Biochemistry, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mentezari@iautmu.ac.ir
Abstract:   (3917 Views)
Background: The aim of this study was to evaluate effect of ubiquinone on MCL1 gene expression in hippocampal CA1 pyramidal cells following transient global ischemia/reperfusion in male Wistar rat.
Materials and methods: In this study, we randomly selected 24 Wistar rats under 250-300 g weights that divided into 4 groups including group of observance (maintenance in the laboratory standard condition), ischemia to close the carotid artery and ischemia for 20 minutes, experiment to obtain CoQ10 dose and again obtain CoQ10 coenzyme, and group of control to obtain CoQ10 coenzyme solvent and ischemia again to obtain CoQ10 coenzyme solvent. CoQ10 coenzyme was injected via intraperitoneal (IP) method. The separated hippocampus from the brain was studied by expressing the changes of MCL1 gene.
Results: The expression of MCL1 anti-apoptic gene in observance group has been decreased compared to experiment group. There were not significant differences in the expression of MCL1 gene in the ischemia group towards control group. The expression of MCL1 gene have no significant differences in the coenzyme of control group towards observance group.
Conclusion: According to the results, CoQ10 can create the protective effects in the severity of lesions of hippocampus after ischemia and also increases the rate of the expression gene.
 
Keywords: CoQ10, Cerebral ischemia, Oxidative stress, Free radicals, Apoptosis, Hippocampus, Real time PCR.
Full-Text [PDF 268 kb]   (1122 Downloads)    
Semi-pilot: Experimental | Subject: Animal Biology
Received: 2018/01/27 | Accepted: 2018/05/16 | Published: 2018/12/23
References
1. Bokura H, Robinson RG. Long-term cognitive impairment associated with caudate stroke. Stroke 1997; 28: 970-5 [DOI:10.1161/01.STR.28.5.970]
2. Braduik B, Sonesson B, Holtas S. Spatial impairment hallowing right hemisphere transient ischemic attacks in patients without carotid artery stenosis. Acta Neural Scand 1989; 80:411-18. [DOI:10.1111/j.1600-0404.1989.tb03902.x]
3. Godefroy O, Rousseaux M, Pruro JP, Cabara M, Leys D. Neuropsychological changes related to unilateral lenticostriate infarcts. J Neurol Neurosurg Psychiat 1994; 57: 480-85. [DOI:10.1136/jnnp.57.4.480]
4. Mustoe T. "Understanding chronic wounds: a unifying hypothesis on their pathogenesis and implications for therapy". Am J Surg. 2004;187: 65S–70S. [DOI:10.1016/S0002-9610(03)00306-4]
5. Hossman KA. Post-ischemic resucitation of the brain :selective vulnerability versus global resistance. Prog Brain Res. 1985;63:3-7. [DOI:10.1016/S0079-6123(08)61971-8]
6. Pulsinelli WA, Brieley JB. A new model of bilateral hemispheric ischemia in the unanesthetized rat. Strok. 1979;10:268-72. [DOI:10.1161/01.STR.10.3.267]
7. Zola-Morgan S, Squire LR, Amaral DG. Human amnesia and the medial temporal region: enduring memory impairment following a bilateral lesion limited to field CA1 of the hippocampus. J Neur. 1986;6: 2950-2967. [DOI:10.1523/JNEUROSCI.06-10-02950.1986]
8. Petito CK, Feldmann E, Pulsinelli WA, Plum F. Delayed hippocampal damage in humans following cardiorespiratory arrest. Neurology. 1987;37: 1281-86. [DOI:10.1212/WNL.37.8.1281]
9. Morioka T, Kalehua AN, Streit WJ. Progressive expression of immunomolecules on microglial cells in rat dorsal hippocampus following transient forbrain ischemia. Acta Neuropathol. 1992;83:149-57. [DOI:10.1007/BF00308474]
10. Collino M, Aragno M, Mastrocola R, Gallicio M, Rosa AC, Dianzani C, et al. Modulation of the oxidative stress and inflammatory response by PPAR-Y agonists in the hippocampus of rats exposed to cerebral ischemia/reperfusion. Eur J Pharmacol. 2006;530:70-80. [DOI:10.1016/j.ejphar.2005.11.049]
11. Block F. Global ischemia and behavioral deficits .Prog Neurobiol. 1999;58:279-95. [DOI:10.1016/S0301-0082(98)00085-9]
12. Fisher M, ed. Stroke therapy. 2nd ed. Boston, USA: Butter Worth Heinemann; 2001. p:25-50.
13. Hachinski V:Relevence of stroke models of stroke. Dis Model Mech. 2012; 5: 718-25 [DOI:10.1242/dmm.010033]
14. Grotta J. The current status of neuronal protective therapy: Why have all neuronal protective drugs worked in animals but none so far in stroke patients ? Cerebrovasc Dis 1994;4:115-120. [DOI:10.1159/000108466]
15. Neuroprotection as initial therapy in acute stroke. Third Report of an Ad Hoc Consensus Group Meeting. The European Ad Hoc Consensus Group. Cerebrovasc Dis. 1998 Jan-Feb;8(1):59-72
16. Won R, Lee KH, Lee BH. Coenzyme Q10 protects neurons against neurotoxicity in hippocampal slice culture. Neuro Report. 2011; 22: 721-25. [DOI:10.1097/WNR.0b013e32834acb8d]
17. Kalayci M, Unal MM, Gul S, Acikgoz S, Kandemir N, Hanci V, et al. Effect of coenzyme Q10 on ischemia and neuronal damage in an experimental traumatic brain-injury model in rats. BMC Neurosci. 2011;12:75. [DOI:10.1186/1471-2202-12-75]
18. Horecký J, Gvozdjáková A, Kucharská J, Obrenovich ME, Palacios HH, Li Y, et al. Effects of coenzyme Q and creatine supplementation on brain energy metabolism in rats exposed to chronic cerebral hypoperfusion. Curr Alzheimer Res. 2011;8:868-75. [DOI:10.2174/156720511798192727]
19. Binukumar BK, Gupta N, Sunkaria A, Kandimalla R, Wani WY, Sharma DR, et al. Protective efficacy of coenzyme Q10 against DDVP-induced cognitive impairments and neurodegeneration in rats. Neurotox Res. 2012;21:345-57. [DOI:10.1007/s12640-011-9289-0]
20. Ishrat T, Khan MB, Hoda MN, Yousuf S, Ahmad M, Ansari MA, et al. Coenzyme Q10 modulates cognitive impairment against intracerebroventricular injection of streptozotocin in rats. Behav Brain Res. 2006;171:9-16. [DOI:10.1016/j.bbr.2006.03.009]
21. Russell T. Matthews, Lichuan Yang, Susan Browne, Myong Baik, and M. Flint Beal. Coenzyme Q10 administration increases brain mitochondrial concentrations and exerts neuroprotective effects. Proc Natl Acad Sci U S A. 1998; 95: 8892–8897. [DOI:10.1073/pnas.95.15.8892]
22. Li H, Klein G, Sun P, Buchan AM. CoQ10 fails to protect brain against focal and global ischemia in rats. Brain Res. 2000;877:7-11. [DOI:10.1016/S0006-8993(00)02609-3]
23. Li G, Zou L, Jack CR Jr, Yang Y, Yang ES. Neuroprotective effect of Coenzyme Q10 on ischemic hemisphere in aged mice with mutations in the amyloid precursor protein. Neurobiol Aging. 2007;28:877-82. [DOI:10.1016/j.neurobiolaging.2006.05.005]
24. Ostrowski RP. Effect of coenzyme Q10 on biochemical and morphological changes in experimental ischemia in the rat brain. Brain Res Bull. 2000;53:399-407. [DOI:10.1016/S0361-9230(00)00406-8]
25. bd-El-Fattah AA, El-Sawalhi MM, Rashed ER, El-Ghazaly MA. Possible role of vitamin E, coenzyme Q10 and rutin in protection against cerebral ischemia/reperfusion injury in irradiated rats. Int J Radiat Biol. 2010;86:1070-8. [DOI:10.3109/09553002.2010.501844]
26. Piotrowski P, Wierzbicka K, Smiałek M. Neuronal death in the rat hippocampus in experimental diabetes and cerebral ischaemia treated with antioxidants. Folia neuropathologica/Association of Polish Neuropathologists and Medical Research Centre. Polish Acad Sci 2001;39: 147-52.
27. Ostrowski R. Effect of coenzyme Q10 (CoQ10) on superoxide dismutase activity in ET-1 and ET-3 experimental models of cerebral ischemia in the rat. Folia neuropathologica/Association of Polish Neuropathologists and Medical Research Centre. Polish Acad Sci 1999;37: 247-51.
28. Spindler M, Beal MF, Henchcliffe C. Coenzyme Q10 effects in neurodegenerative disease. Neuropsychiatr Dis Treat. 2009;5:597-610.
29. Quinzii CM, Hirano M. Coenzyme Q and mitochondrial disease. Dev Disabil Res Rev. 2010;16:183-38. [DOI:10.1002/ddrr.108]
30. Young AJ, Johnson S, Steffens DC, Doraiswamy PM. Coenzyme Q10: a review of its promise as a neuroprotectant. CNS Spectr. 2007;12:62-68. [DOI:10.1017/S1092852900020538]
31. Zamani M, Mehdizadeh M, Mohamadzadeh F, Soleimani M. Coenzyme Q10 protects hippocampal neurons against ischemia/ reperfusion injury via modulation of BAX/Bcl-2 expression. Basic Clin Neur. 2012;3:5-10.
32. Khazaei Koohpar Z, Entezari M, Movafagh A, Hashemi M. Anticancer Activity of Curcumin on Human Breast Adenocarcinoma: Role of Mcl-1 Gene. Iran J Cancer Prev. 2015;8:1-4. [DOI:10.17795/ijcp2331]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA

XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Shirvani Shahenayati M, Entezari M. The effects of ubiquinone antioxidants on the MCL1 gene in the CA1 pyramidal cells of hippocampus of male Wistar rat following transient reperfusion inclusive ischemia . MEDICAL SCIENCES 2018; 28 (4) :270-276
URL: http://tmuj.iautmu.ac.ir/article-1-1486-en.html
Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 28, Issue 4 (winter 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.07 seconds with 37 queries by YEKTAWEB 4645