[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 29, Issue 1 (spring 2019) ::
MEDICAL SCIENCES 2019, 29(1): 56-63 Back to browse issues page
Evaluating the presentation of blaCTX-M, blaTEM, and blaSHV resistance genes in Escherichia coli isolated from animal food sources in Tonekabon city and determination of their antibiotic resistance profile
Shiva Khajavi1 , Zoheir Heshmatipour * 2, Akram Sadat Tabatabaee Bafroee3
1- MSc, Department of microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran
2- Department of microbiology, Tonekabon branch, Islamic Azad University, Tonekabon, Iran , zheshmat@gmail.com
3- Department of Biology, East Tehran Branch, Islamic Azad University, Tehran, Iran
Abstract:   (116 Views)
Background: prevalence of E. coli which produces broad spectrum Beta-lactamases in the category of animals like sheep as well as the risk of its transmission to humans have become a significant concern during recent years. The purpose of the research was to study the presence of genes blaCTX-M , blaTEM  and blaSHV in E.coli isolates derived from lamb meat in butcheries of Tonekabon city, and to determine the anti-biotic resistance pattern of these isolates.
Materials and methods: 50 samples of lamb meat were randomly collected from butcheries of Tonekabon city and were transferred to laboratory under sterile conditions. The presence of studied genes was examined using PCR technique and proprietary primers. Anti-biotic sensitivity test was conducted using Kirby’s disk diffusion method.
Results: Among obtained isolates, 30 isolates were identified as E. coli. All 30 isolated (100%) carried the blaCTX-M gene and lacked blaSHV. blaCTX-M was identified in 7 isolates (23.3%). 7 samples (23.3%) were positive for both blaCTX-M and blaTFM. The analysis of anti-biotic resistance showed that the isolates were resistant against amoxicillin-clavulanic (86/7%), doxycycline and cephadol (10%) and tetracycline (6/7%). On the other hand, all 30 isolates (100%) were susceptible to cefotaxime and ceftazidime, and 28 isolates were susceptible to ceftriaxone.
Conclusion: Isolates susceptible to antibiotics which carrying resistant genes, probably have the potential to become resistant against them. Thus logical usage of antibiotics should be considered more than before.
Keywords: E.coli, blaCTX-M, bla TEM, bla SHV, Animal food sources
Full-Text [PDF 1530 kb]   (59 Downloads)    
Semi-pilot: Quazi-Experimental | Subject: Microbiology
Received: 2018/05/20 | Accepted: 2018/09/3 | Published: 2019/03/11
References
1. Martinez JL. Environmental pollution by antibiotics and by antibiotic resistance determinants. Environ Pollut 2009;157:2893-902. [DOI:10.1016/j.envpol.2009.05.051] [PMID]
2. Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev 2010;74:417-33. [DOI:10.1128/MMBR.00016-10] [PMID] [PMCID]
3. Sallem RB, Slama KB, Estepa V, Jouini A, Gharsa H, Klibi N, et al. Prevalence and characterisation of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in healthy volunteers in Tunisia. Eur J Clin Microbiol Infect Dis 2012;31:1511-6. [DOI:10.1007/s10096-011-1471-z] [PMID]
4. Geser N, Stephan R, Hächler H. Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Vet Res 2012;8:1. [DOI:10.1186/1746-6148-8-21] [PMID] [PMCID]
5. Ewers C, Bethe A, Semmler T, Guenther S, Wieler LH. Extended‐spectrum β‐lactamase‐producing and AmpC‐producing Escherichia coli from livestock and companion animals, and their putative impact on public health: a global perspective. Clin Microbiol Infect 2012;18:646-55. [DOI:10.1111/j.1469-0691.2012.03850.x] [PMID]
6. Branger C, Zamfir O, Geoffroy S, Laurans G, Arlet G, Thien HV, et al. Genetic background of Escherichia coli and extended-spectrum beta-lactamase type. Emerg Infect Dis 2005;11:54-61. [DOI:10.3201/eid1101.040257] [PMID] [PMCID]
7. Shaikh S, Fatima J, Shakil S, Rizvi SMD, Kamal MA. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi J Biol Sci 2015;22:90-101. [DOI:10.1016/j.sjbs.2014.08.002] [PMID] [PMCID]
8. Livermore D. Defining an extended‐spectrum β‐lactamase. Clin Microbiol Infect 2008;14:3-10. [DOI:10.1111/j.1469-0691.2007.01857.x] [PMID]
9. Bush K. Extended‐spectrum β‐lactamases in North America, 1987-2006. Clin Microbiol Infect 2008;14:134-43. [DOI:10.1111/j.1469-0691.2007.01848.x] [PMID]
10. Melvin P. Performance Standards for Antimicrobial Susceptibility Testing. 27th ed. 2017.
11. Carattoli A. Animal reservoirs for extended spectrum β‐lactamase producers. Clin Microbiol Infect 2008;14:117-23. [DOI:10.1111/j.1469-0691.2007.01851.x] [PMID]
12. Petternel CH, Galler H, Zarfel G, Luxner J, Haas D, Grisold A. Isolation bacteria from minced meat in Asteria. 2014.
13. Agersø Y, Aarestrup FM, Pedersen K, Seyfarth AM, Struve T, Hasman H. Prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in Danish slaughter pigs and retail meat identified by selective enrichment and association with cephalosporin usage. J Antimicrob Chemother 2012;67:582-8. [DOI:10.1093/jac/dkr507] [PMID]
14. Bonyadian M, Ebrahimi A, Jamali M. Study on the antibiotic resistance of E.coli isolated from raw milk and unpasteurized cheese and survey on resistance transmission to E.coli O2:K12. J Vet Clin Sci 2013;7:25-31.
15. Haghighi KP, Ali Nezhad I. Antibacterial resistance patterns of Escherichia coli isolated from broilers coli bacillosis of broilers chicks in golestan province. J Vet Clin Res 2010;1:39-47.
16. Schmid A, Hörmansdorfer S, Messelhäusser U, Käsbohrer A, Sauter-Louis C, Mansfeld R. Prevalence of extended-spectrum β-lactamase-producing Escherichia coli on Bavarian dairy and beef cattle farms. Appl Environ Microbiol 2013;7:3027-32. [DOI:10.1128/AEM.00204-13] [PMID] [PMCID]
17. Kala A, Kahler C, Pfeifer Y, Schwab F, Kuhn K, Balau V, et al. High prevalence of extended-spectrum-beta-lactamase producing Entrobacteriaceae in organic and conventional retail chicken meat, Germany. J Antimicrob Chemother 2012;67:2631-4. [DOI:10.1093/jac/dks295] [PMID]
18. Yazdi M, Nazemi A, Mirinargasi M, Jafarpour M, Sharifi SH. Genotypic versus Phenotypic methods to detect Extended-Spectrum Beta-Lactamases (ESBLs) in Uropathogenic Escherichia coli. Annals Biol Res 2012;3:2454-8.
19. Martınez JL, Rojo F. Metabolic regulation ofantibiotic resistance. FEMS Microbiol Rev 2011;35:768-89. [DOI:10.1111/j.1574-6976.2011.00282.x] [PMID]
20. O'Neil AJ. 'Silent' antibiotic resistance genes: an overlooked issue of considerable importance in antibacterial chemotherapy, 2017.
21. Enne VI, A. Delsol A, M. Roe J, M. Bennett P. Evidence of Antibiotic Resistance Gene Silencing in Escherichia coli. Antimicrob Agents Chemother 2006;50:3003-10. [DOI:10.1128/AAC.00137-06] [PMID] [PMCID]
22. Martinez JL. Environmental pollution by antibiotics and by antibiotic resistance determinants. Environ Pollut 2009;157:2893-902. [DOI:10.1016/j.envpol.2009.05.051] [PMID]
23. Davies J, Davies D. Origins and evolution of antibiotic resistance. Microbiol Mol Biol Rev 2010;74:417-33. [DOI:10.1128/MMBR.00016-10] [PMID] [PMCID]
24. Sallem RB, Slama KB, Estepa V, Jouini A, Gharsa H, Klibi N, et al. Prevalence and characterisation of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in healthy volunteers in Tunisia. Eur J Clin Microbiol Infect Dis 2012;31:1511-6. [DOI:10.1007/s10096-011-1471-z] [PMID]
25. Geser N, Stephan R, Hächler H. Occurrence and characteristics of extended-spectrum β-lactamase (ESBL) producing Enterobacteriaceae in food producing animals, minced meat and raw milk. BMC Vet Res 2012;8:1. [DOI:10.1186/1746-6148-8-21] [PMID] [PMCID]
26. Ewers C, Bethe A, Semmler T, Guenther S, Wieler LH. Extended‐spectrum β‐lactamase‐producing and AmpC‐producing Escherichia coli from livestock and companion animals, and their putative impact on public health: a global perspective. Clin Microbiol Infect 2012;18:646-55. [DOI:10.1111/j.1469-0691.2012.03850.x] [PMID]
27. Branger C, Zamfir O, Geoffroy S, Laurans G, Arlet G, Thien HV, et al. Genetic background of Escherichia coli and extended-spectrum beta-lactamase type. Emerg Infect Dis 2005;11:54-61. [DOI:10.3201/eid1101.040257] [PMID] [PMCID]
28. Shaikh S, Fatima J, Shakil S, Rizvi SMD, Kamal MA. Antibiotic resistance and extended spectrum beta-lactamases: Types, epidemiology and treatment. Saudi J Biol Sci 2015;22:90-101. [DOI:10.1016/j.sjbs.2014.08.002] [PMID] [PMCID]
29. Livermore D. Defining an extended‐spectrum β‐lactamase. Clin Microbiol Infect 2008;14:3-10. [DOI:10.1111/j.1469-0691.2007.01857.x] [PMID]
30. Bush K. Extended‐spectrum β‐lactamases in North America, 1987-2006. Clin Microbiol Infect 2008;14:134-43. [DOI:10.1111/j.1469-0691.2007.01848.x] [PMID]
31. Melvin P. Performance Standards for Antimicrobial Susceptibility Testing. 27th ed. 2017.
32. Carattoli A. Animal reservoirs for extended spectrum β‐lactamase producers. Clin Microbiol Infect 2008;14:117-23. [DOI:10.1111/j.1469-0691.2007.01851.x] [PMID]
33. Petternel CH, Galler H, Zarfel G, Luxner J, Haas D, Grisold A. Isolation bacteria from minced meat in Asteria. 2014.
34. Agersø Y, Aarestrup FM, Pedersen K, Seyfarth AM, Struve T, Hasman H. Prevalence of extended-spectrum cephalosporinase (ESC)-producing Escherichia coli in Danish slaughter pigs and retail meat identified by selective enrichment and association with cephalosporin usage. J Antimicrob Chemother 2012;67:582-8. [DOI:10.1093/jac/dkr507] [PMID]
35. Bonyadian M, Ebrahimi A, Jamali M. Study on the antibiotic resistance of E.coli isolated from raw milk and unpasteurized cheese and survey on resistance transmission to E.coli O2:K12. J Vet Clin Sci 2013;7:25-31.
36. Haghighi KP, Ali Nezhad I. Antibacterial resistance patterns of Escherichia coli isolated from broilers coli bacillosis of broilers chicks in golestan province. J Vet Clin Res 2010;1:39-47.
37. Schmid A, Hörmansdorfer S, Messelhäusser U, Käsbohrer A, Sauter-Louis C, Mansfeld R. Prevalence of extended-spectrum β-lactamase-producing Escherichia coli on Bavarian dairy and beef cattle farms. Appl Environ Microbiol 2013;7:3027-32. [DOI:10.1128/AEM.00204-13] [PMID] [PMCID]
38. Kala A, Kahler C, Pfeifer Y, Schwab F, Kuhn K, Balau V, et al. High prevalence of extended-spectrum-beta-lactamase producing Entrobacteriaceae in organic and conventional retail chicken meat, Germany. J Antimicrob Chemother 2012;67:2631-4. [DOI:10.1093/jac/dks295] [PMID]
39. Yazdi M, Nazemi A, Mirinargasi M, Jafarpour M, Sharifi SH. Genotypic versus Phenotypic methods to detect Extended-Spectrum Beta-Lactamases (ESBLs) in Uropathogenic Escherichia coli. Annals Biol Res 2012;3:2454-8.
40. Martınez JL, Rojo F. Metabolic regulation ofantibiotic resistance. FEMS Microbiol Rev 2011;35:768-89. [DOI:10.1111/j.1574-6976.2011.00282.x] [PMID]
41. O'Neil AJ. 'Silent' antibiotic resistance genes: an overlooked issue of considerable importance in antibacterial chemotherapy, 2017.
42. Enne VI, A. Delsol A, M. Roe J, M. Bennett P. Evidence of Antibiotic Resistance Gene Silencing in Escherichia coli. Antimicrob Agents Chemother 2006;50:3003-10. [DOI:10.1128/AAC.00137-06] [PMID] [PMCID]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Khajavi S, Heshmatipour Z, Tabatabaee Bafroee A S. Evaluating the presentation of blaCTX-M, blaTEM, and blaSHV resistance genes in Escherichia coli isolated from animal food sources in Tonekabon city and determination of their antibiotic resistance profile . MEDICAL SCIENCES. 2019; 29 (1) :56-63
URL: http://tmuj.iautmu.ac.ir/article-1-1531-en.html


Volume 29, Issue 1 (spring 2019) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3877