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Detection of IL-10 gene polymorphisms in patients with ulcerative colitis in Northern Iran
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Mahsa Naeimi1   , Rasoul Zahmatkesh Roudsari  2 |
1- Master Student, Department of Cell and Molecular Sciences, School of Biology Sciences, Islamic Azad University Tonekabon Branch, Tonekabon, Iran
2- Department of Cell and Molecular Sciences, School of Biology Sciences, Islamic Azad University Tonekabon Branch, Tonekabon, Iran , rasoul130@yahoo.com |
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Abstract: (4087 Views) |
Background: Ulcerative colitis is a multifactorial disease in which the environmental and genetics factor are being involved together. Interleukins are a group of cytokine which are produced through T cells, monocytes, macrophages, and endothelial cells. Interleukin-10 is an important multitasking cytokine that has a key role in inflammatory responses. The promoter of interlekin-10 is highly polymorphic and many of its polymorphisms were identified. The purpose of this study was to investigate and detect the A1082G and T819C polymorphisms of interleukin-10 promoter in ulcerative colitis patients.
Materials and methods: In this case- control study, DNA from 150 individuals with ulcerative colitis and 150 controls were determined by PCR technique. Data were analyzed by Chi-square test using SPSS software. Also, odds ratios and 95% confidence interval were calculated.
Results: The results showed that distribution of GG and CC genotypes and frequency of G and C alleles (related to A1082C and T819C polymorphisms, respectively) was significantly different between patient and control groups.
Conclusion: According to the results, both polymorphisms of IL-10 gene promoter (A1082G and T819C), including G allel and C allel, respectively, are likely to be considered as risk factors for ulcerative colitis. Moreover, both genotype of GG and CC can increase the incidence of ulcerative colitis.
Keywords: Ulcerative colitis, Polymorphism, IL-10 (A1082G), IL-10 (T819C). |
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| Keywords: Ulcerative colitis, Polymorphism, IL-10 (A1082G), IL-10 (T819C). |
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Full-Text [PDF 695 kb]
(1396 Downloads)
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Semi-pilot: case-control |
Subject:
Biology
Received: 2018/09/15 | Accepted: 2018/11/23 | Published: 2019/06/16
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