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:: Volume 29, Issue 4 ( winter 2019) ::
MEDICAL SCIENCES 2019, 29(4): 296-302 Back to browse issues page
Bad gene expression following effect of coenzyme Q10 on Wistar rat hippocampus with cerebral ischemia
Farnoosh Kazemi Asafeh1, Maliheh Entezari * 2, Maryam Shirvani Shahenayati1
1- MSc in Molecular Genetics, Department of Biology, Islamic Azad University, Damghan Branch, Damghan, Iran
2- Associate Professor, Department of genetics, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mentezari@iautmu.ac.ir
Abstract:   (222 Views)
Background: Q10 coenzyme is a potent antioxidant in the mitochondrial membrane. Releasing the oxygen free radicals occurs in the cerebral ischemia. Using Q10 coenzyme causes strength against oxidative after injury of cerebral ischemia during reperfusion. Also CoQ10 plays an important anti-apoptotic role to reduce Caspase 3 as a key enzyme neuroprotective in apoptosis. According to the sensitive nature of CA1-Hypocampus pyramidal neurons against the cerebral ischemia, we studied the effects of CoQ10 in this area of rat`s brain after transient general ischemia/ reperfusion.
Materials and methods: We selected randomly 24 heads of Wistar rats under 250-300 g weights that divided into 4 groups, including control, experiment (ischemia, Q10), ischemia and Q10 coenzyme groups. Then we separated the hippocampus from the brain expression of pro-apoptosis genes was studied. After extraction of RNA and synthesis of cDNA, we used real time RCR methods to express this gene.
Results: The expression of Bad gene in experiment group (Q10, 10mg/Kg) significantly decreased compared to ischemia group (p<0.05).
Conclusion: It seems that ischemia caused to increase the expression of Bad pro-apoptic gene, and CoQ10 with dose of 10mg/Kg has effects on the expression of Bad gene and prevent the severity of lesions.
Keywords: CoQ10, Apoptosis, Hippocampus, Real Time PCR.
Full-Text [PDF 417 kb]   (74 Downloads)    
Semi-pilot: Experimental | Subject: Animal Biology
Received: 2018/12/3 | Accepted: 2019/02/26 | Published: 2019/12/28
1. 1. Badr R, Hashemi M, Javadi G, Movafagh A, Mahdian R. Assessment of global ischemic /reperfusion and Tacrolimus administration on CA1 region of hippocampus: gene expression profiles of BAX and BCL2 genes. Bratisl Lek Listy 2016;117:358-62. [DOI:10.4149/BLL_2016_071]
2. Badr R, Hashemi M, Javadi G, Movafagh A, Mahdian R. Gene Expression ‌‌‌‌Profiles of BAD and Bcl-xL in the CA1 Region of the Hippocampus Following Global Ischemic/Reperfusion and FK-506 Administration. Iran Red Crescent Med J 2015;17:e23145. [DOI:10.5812/ircmj.23145]
3. Behroozaghdam M, Hashemi M, Javadi G, Mahdian R, Soleimani M. Expression of bax and bcl2 Genes in MDMA-induced Hepatotoxicity on Rat Liver Using Quantitative Real-Time PCR Method through Triggering Programmed Cell Death. Iran Red Crescent Med J 2015; 26:17:e24609. [DOI:10.5812/ircmj.24609]
4. Ishrat T, Khan MB, Hoda MN, Yousuf S, Ahmad M, Ansari MA, et al. Coenzyme Q10 modulates cognitive impairment against intracerebroventricular injection of streptozotocin in rats. Behav Brain Res 2006; 171: 9-16. [DOI:10.1016/j.bbr.2006.03.009]
5. Gholnari T, Aghadavod E, Soleimani A, Hamidi GA, Sharifi N, Asemi Z. The Effects of Coenzyme Q10 Supplementation on Glucose Metabolism, Lipid Profiles, Inflammation, and Oxidative Stress in Patients With Diabetic Nephropathy: A Randomized, Double-Blind, Placebo-Controlled Trial. J Am Coll Nutr 2018;37:188-93. [DOI:10.1080/07315724.2017.1386140]
6. Awad AM, Bradley MC, Fernández-Del-Río L, Nag A, Tsui HS, Clarke CF. Coenzyme Q10 deficiencies: pathways in yeast and humans. Essays Biochem 2018;62:361-376. [DOI:10.1042/EBC20170106]
7. Kalayci M, Unal MM, Gul S, Acikgoz S, Kandemir N, Hanci V, et al. Effect of Coenzyme Q10 on ischemia and neuronal damage in an experimental traumatic brain-injury model in rats. BMC neuroscience. BMC Neurosci 2011;12:75. [DOI:10.1186/1471-2202-12-75]
8. Horecký J, Gvozdjáková A, Kucharská J, Obrenovich ME, Palacios HH, Li Y, et al. Effects of Coenzyme Q and Creatine Supplementation on Brain Energy Metabolism in Rats Exposed to Chronic Cerebral Hypoperfusion. Curr Alzheimer Res 2011; 8: 868-875. [DOI:10.2174/156720511798192727]
9. Young AJ, Johnson S, Steffens DC, Doraiswamy PM. Coenzyme Q10: a review of its promise as a neuroprotectant. CNS spectrums 2007; 12: 62-68. [DOI:10.1017/S1092852900020538]
10. Hashemi M. The Study of Pentoxifylline Drug Effects on Renal Apoptosis and BCL-2 Gene Expression Changes Following Ischemic Reperfusion Injury in Rat. Iran J Pharm Res 2014; 13: 181-89.
11. Farahani K, Hashemi M. Investigating the Effect of Hydroalcoholic Extract of Cyperus rotundus L. on the Expression of Bcl-x1 Antiapoptotic Gene in Rats' Hippocampus Tissue Following Global Ischemic-Reperfusion Injury. Acta Medica Iranica 2016;54:256-60.
12. Khazaei Koohpar Z, Entezari M, Movafagh A, Hashemi M. Anticancer activity of curcumin on human breast adenocarcinoma: Role of Mcl-1 gene. Iran J Cancer Prev 2015;8:1-4. [DOI:10.17795/ijcp2331]
13. Sharifi ZN, Abolhassani F, Zarrindast MR, Movassaghi S, Rahimian N, Hassanzadeh G. Effects of FK506 on hippocampal CA1 cells following transient global ischemia/reperfusion in Wister rat. Stroke Res Treat 2012;2012:809417. [DOI:10.1155/2012/809417]
14. Sari S, Hashemi M, Mahdian R, Parivar K, Rezayat M. The effect of pentoxifylline on bcl-2 gene expression changes in hippocampus after ischemia-reperfusion in Wistar rats by a quatitative RT-PCR method. Iran J Pharm Res 2013; 12: 495-501.
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Kazemi Asafeh F, Entezari M, Shirvani Shahenayati M. Bad gene expression following effect of coenzyme Q10 on Wistar rat hippocampus with cerebral ischemia. MEDICAL SCIENCES. 2019; 29 (4) :296-302
URL: http://tmuj.iautmu.ac.ir/article-1-1683-en.html

Volume 29, Issue 4 ( winter 2019) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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