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:: Volume 29, Issue 4 ( winter 2019) ::
MEDICAL SCIENCES 2019, 29(4): 303-312 Back to browse issues page
The effect of charantin on expression of Ngn3 gene in streptozotocin-induced diabetic rats
Abolfazl Nasirzadeh Vanhari1, Hossein Sazgar * 2
1- MSc in Biology and Biochemistry, Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
2- Assistant Professor of Physiology, Department of Biology, Faculty of Basic Sciences, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran , hoseinsazgar@yahoo.com
Abstract:   (265 Views)
Background: Positive effects of medicinal herbs on diabetes have been demonstrated in previous studies. The aim of the present study was to evaluate the effect of charantin on the treatment of diabetes and increase the expression of the Ngn3 gene in diabetes, which is a key gene for transcription of the pancreas.
Materials and methods: In this experimental study, 42 adult male Wistar rats were randomly divided into 7 healthy control groups, diabetic control, control group, 150 control, and three groups receiving effective doses of charantin (50-100-200 mg/ Per kilogram). All groups except diabetic control group and control group with streptozotocin chemical injected intraperitoneally. Afterwards, they received a cartilage by gavage for four weeks (three times a week).
Results: The lowest expression was observed in the diabetic group. Among those who received different doses of charantine, the highest expression was among group with dose of 100 mg/kg, and the lowest expression in 50/kg group.
Conclusion: It can be concluded that increased expression of the Ngn3 gene was not dose-dependent. After the development of diabetes, the patient's severity declined sharply, and after the use of the charantin and metformin drug, this reduction was somewhat offset.
Keywords: Diabetes, Streptozotocin, Charantin, Ngn3 gene
Full-Text [PDF 425 kb]   (78 Downloads)    
Semi-pilot: Experimental | Subject: Physiology
Received: 2018/05/20 | Accepted: 2018/09/3 | Published: 2019/12/28
1. 1. Meusel, L, Kansal, N, Tchistiakova, E, Yuen W, MacIntosh, B, Greenwood, C, et al. A systematic review of type 2 diabetes mellitus and hypertension in imaging studies of cognitive aging: time to establish new norms. Front Aging Neurosci 2014.6: 148. [DOI:10.3389/fnagi.2014.00148]
2. Patel DK, Kumar R, Prasad SK, Sairam K, Hemalatha S. Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus (Linn) F. Muell in streptozotocin-induced diabetic rats. Asian Pac J Trop Biomed 2011;1: 316-322. [DOI:10.1016/S2221-1691(11)60051-8]
3. Scherbaum WA. Insulin therapy in Europe-Diabetes. Metab Res Rev 2002; 3: 3:S50-6. [DOI:10.1002/dmrr.298]
4. Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract 2010;87:4-14. [DOI:10.1016/j.diabres.2009.10.007]
5. Nolte MS, Karam JH, ed. Pancreatic hormones and antidiabetic drugs. Basic and clinical pharmacology. 9th ed. New York: McGraw Hill Companies; 2004. P.708.
6. Hald A, Lotharius J. Oxidative stress and inflammation in Parkinson's disease: is there a causal link? Exp Neurol 2005;193:279-90. [DOI:10.1016/j.expneurol.2005.01.013]
7. Venkatesh S, Reddy GD, Reddy BM, Ramesh M, Rao AV. Antihyperglycemic activity of Caralluma attenuata. Fitoterapia 2003; 74:274-9. [DOI:10.1016/S0367-326X(03)00021-2]
8. McCune LM, Johns T. Antioxidant activity in medicinal plants associated with the symptoms of diabetes mellitus used by the indigenous peoples of the North American boreal forest. J Ethnopharmacol 2002;82:197-205. [DOI:10.1016/S0378-8741(02)00180-0]
9. Fallah Huseini H, Fakhrzadeh H, Larijani B, Shikh Samani A. Review of anti-diabetic medicinal plant used in traditional medicine. J Med Plants 2006; 1 :1-8 [In Persian]
10. Hajinejad MR, Salehi Moghadam M, Hajian Shahri S, Vaezi E, Jamshidian A, Sa'atati D. Effect of Carla leaf extract on serum glucose, lipid and malondialdehyde levels in streptozocin-induced diabetic rats. Diabetes Nur 2014;2:8-19. [In Persian]
11. Ahmed I, Adeghate E, Cummings E, Sharma AK, Singh J. Beneficial effects and mechanism of action of Momordica charantia juice in the treatment of streptozotocin-induced diabetes mellitus in rat. Mol Cell Biochem 2004;261:63-70. [DOI:10.1023/B:MCBI.0000028738.95518.90]
12. Yibchok-anun S, Adisakwattana S, Yao CY, Sangvanich P, oengsumran S, Hsu WH. Slow acting protein extract from fruit pulp of Momordica charantia with insulin secretagogue and insulinomimetic activities. Biol Pharm Bull 2006;29:1126-31. [DOI:10.1248/bpb.29.1126]
13. Huang QY, Cheng MR, Ji SL. Linkage and association studies of the susceptibility genes for type 2 diabetes. Yi Chuan Xue Bao 2006; 33:573-89. [DOI:10.1016/S0379-4172(06)60087-5]
14. Patlak M. New weapons to combat at ancient disease: treating diabetes. FASEB J. 2002:16:1853. [DOI:10.1096/fasebj.16.14.1853e]
15. Qiu Li, Zhi-Chun Lai. Recent progress in studies of factors that elicit pancreatic β-cell expansion. Protein Cell 2015; 6: 81-87. [DOI:10.1007/s13238-014-0123-3]
16. Rukstalis JM, Habener JF. Neurogenin3: a master regulator of pancreatic islet differentiation and regeneration. Islets 2009;1:177-184. [DOI:10.4161/isl.1.3.9877]
17. Gomez DL, O'Driscoll M, Sheets TP, Hruban RH, Oberholzer J, McGarrigle JJ, et al. Neurogenin 3 expressing cells in the human exocrine pancreas have the capacity for endocrine cell fate. PLoS One 2015;10:e0133862. doi:10.1371/journal.pone.0133862. [DOI:10.1371/journal.pone.0133862]
18. Pagliuca FW, Melton DA. Melton. How to make a functional β-cell. Development 2013;140:2472-83. [DOI:10.1242/dev.093187]
19. Khaleghi S, Bahrami G, Mahmoodi M, Asgari V, Mostafaie A. Hypoglycemic Effect of Hydroalcoholic Extract and Hexane Fraction of Persian Shallot (Allium Hirtifolium Boiss) Extract in Streptozotocin-Induced Diabetic Rats. JRPS. 2015; 5: 33-40.
20. Kermany H, Shahanipour K, Nakhaee AR. Effect of aqueous and methanolic extracts of Momordica charantia fruit on blood glucose and liver enzymes in diabetic rats. Quarterly of the Horizon of Medical Sciences 2015; 21:105-112. [In Persian] [DOI:10.18869/acadpub.hms.21.2.105]
21. Abdollahi M, Zuki ABZ, Goh YM, Rezaeizadeh A, Noordin MM. The effects of Momordica charantia on the liver in streptozotocin-induced diabetes in neonatal rats. Afr J Biotechnol 2011;26:13-21.
22. Ahangaran, A, Mosahebi Mohammadi G, Koohi Habibi M, Khezri S, Shahraeen, N. Use of rapid serological and nucleic acid-based methods for detecting the soybean mosaic virus. J Agric Sci Technol 2009.11: 91-97.
23. Al-said MS, Abdelsattar EA, Khalifa SI, EI- Feraly FS. Isolation and identification of an anti- inflammatory principle from Capparis spinosa. Pharmazie 1988; 43: 640-41.
24. Lenzen S. The mechanisms of alloxan and STZ- induced diabetes. Diabetologia 2008;51:216-226. [DOI:10.1007/s00125-007-0886-7]
25. Rethod NR. Chime HR. Hypoglycemic effect of calotropis gigantean linn.Leaves and flowers in STZ-diabetic rats. J Oman Med 2011; 26:104-108. [DOI:10.5001/omj.2011.26]
26. Ardestani A, Yazdanparast R, Jamshidi Sh. Therapeutic effects of Teucrium polium extract on oxidative stress in pancreas of streptozotocin-induced diabetic rats. Med Food 2008; 11: 525-32. [DOI:10.1089/jmf.2006.0230]
27. Kavalali G. Tuncel H. Hatmi HH. Hypoglycemic activity of Urtica pilulifera in pilulifera in STZ-diabetic rats. J Ethnopharmacol 2003;84:241-245. [DOI:10.1016/S0378-8741(02)00315-X]
28. Baboota, S. Kohli, K. Ahmad, S. Ali, J. Silymarin: a review of Pharmalogical aspects and bioavilability enhancement approaches. Indian J Pharamacol 2007; 39:172-179. [DOI:10.4103/0253-7613.36534]
29. Kaushik U, Aeri V, Mir SR. Cucurbitacins - An insight into medicinal leads from nature. Pharmacogn Rev 2015;9:12-8. [DOI:10.4103/0973-7847.156314]
30. Hussain F, Tahira S. Antidiabetic evaluation of Momordica charantia L fruit extracts. West Indian Med J 2014; 63:294-99.
31. Gheibi S, Bakhtiarzadeh F, Ghasemi A. A review of high fat diet-streptozotocin model for induction of type 2 diabetes in rat. Iranian Journal of Endocrinology and Metabolism 2016; 18:135-148. [In Persian]
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Nasirzadeh Vanhari A, Sazgar H. The effect of charantin on expression of Ngn3 gene in streptozotocin-induced diabetic rats. MEDICAL SCIENCES. 2019; 29 (4) :303-312
URL: http://tmuj.iautmu.ac.ir/article-1-1684-en.html

Volume 29, Issue 4 ( winter 2019) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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