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:: Volume 29, Issue 4 ( winter 2019) ::
MEDICAL SCIENCES 2019, 29(4): 313-321 Back to browse issues page
The prevalence of Mycoplasma pneumoniae infection in patients with ventilator-associated pneumonia hospitalized in intensive care unit
Shirin Mafi1 , Fatemeh Sakhaee2 , Mohsen Zargar3 , Farzam Vaziri2 , Masoud Zarei4 , Fatemeh Rahimi Jamnani2 , Seyed Davar Siadat2 , Abolfazl Fateh * 5
1- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran
3- Faculty of Science, Qom Branch, Islamic Azad University, Qom, Iran
4- Molecular Laboratory of Erfan Niyayesh Hospital, Tehran, Iran
5- Department of Mycobacteriology and Pulmonary Research, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran , afateh2@gmail.com
Abstract:   (284 Views)
Background: Ventilator-associated pneumonia (VAP) is a type of hospital acquired pneumonia with the mortality rate between 27% and 76% that develops more than 48–72 h after endotracheal intubation. Possible causes leading to this infection can be Mycoplasma pneumoniae. The objective of this study was to determine the presence of Mycoplasma pneumoniae in bronchoalveolar samples of patients with VAP who admitted in the intensive care unit (ICU).
Materials and methods: In this cross-sectional study, 103 bronchoalveolar lavage samples were collected from VAP patients who admitted to ICU in Loghman, Erfan Niyayesh and Erfan Hospitals. Then, samples were investigated for presence of Mycoplasma pneumoniae using nested-PCR method with 16S rRNA gene.
Results: 4 (3.9%) patients were positive for Mycoplasma pneumoniae. There was a significant relationship between the positive infectious agents with clinical signs (P=0.017) and the duration of using ventilator (P=0.043).
Conclusion: The results of this study, as a first study in Iran, showed that Mycoplasma pneumoniae can be considered as true pathogens of the respiratory tract in patients with VAP and should be given more attention. To confirm the results of this study, further research is needed to reveal the association of this bacterium with VAP
Keywords: Ventilator-associated pneumonia, Mycoplasma pneumoniae, 16S rRNA gene
Full-Text [PDF 345 kb]   (79 Downloads)    
Semi-pilot: Survey/Cross Sectional/Descriptive | Subject: Microbiology
Received: 2019/03/16 | Accepted: 2019/05/11 | Published: 2019/12/28
References
1. 1. Kalil AC, Metersky ML, Klompas M, Muscedere J, Sweeney DA, Palmer LB, et al. Management of adults with hospital-acquired and ventilator-associated pneumonia: 2016 clinical practice guidelines by the Infectious Diseases Society of America and the American Thoracic Society. Clin Infect Dis 2016;63: 61-111. [DOI:10.1093/cid/ciw353]
2. Othman AA, Abdelazim MS. Ventilator-associated pneumonia in adult intensive care unit prevalence and complications. The Egyptian Journal of Critical Care Medicine 2017;5:61-3. [DOI:10.1016/j.ejccm.2017.06.001]
3. Gadani H, Vyas A, Kar AK. A study of ventilator-associated pneumonia: Incidence, outcome, risk factors and measures to be taken for prevention. Indian J Anaesth 2010;54:535-40. [DOI:10.4103/0019-5049.72643]
4. Kalanuria AA, Zai W, Mirski M. Ventilator-associated pneumonia in the ICU. Critical care 2014;18:208. [DOI:10.1186/cc13775]
5. Muscedere JG, Day A, Heyland DK. Mortality, attributable mortality, and clinical events as end points for clinical trials of ventilator-associated pneumonia and hospital-acquired pneumonia. Clin Infect Dis 2010;51: 120-25. [DOI:10.1086/653060]
6. Mokhless NA-S, El-Mofty MF, Hanafi NF, Muhammad A. Atypical bacteria in ventilator associated pneumonia; an Egyptian University Hospital experience. J Am Sci 2010;6:1074-79.
7. Saraya T. Mycoplasma pneumoniae infection: Basics. J Gen Fam Med 2017;18:118-25. [DOI:10.1002/jgf2.15]
8. Meyer Sauteur PM, Unger WW, Nadal D, Berger C, Vink C, van Rossum A. Infection with and Carriage of Mycoplasma pneumoniae in Children. Front Microbiol 2016; 23: 329. [DOI:10.3389/fmicb.2016.00329]
9. Atkinson TP, Balish MF, Waites KB. Epidemiology, clinical manifestations, pathogenesis and laboratory detection of Mycoplasma pneumoniae infections. FEMS Microbiol Rev 2008;32:956-73. [DOI:10.1111/j.1574-6976.2008.00129.x]
10. Saraya T. The history of Mycoplasma pneumoniae pneumonia. Front Microbiol 2016;7:364. [DOI:10.3389/fmicb.2016.00364]
11. Principi N, Esposito S, Blasi F, Allegra L, Group MS. Role of Mycoplasma pneumoniae and Chlamydia pneumoniae in children with community-acquired lower respiratory tract infections. Clin Infect Dis 2001;32:1281-9. [DOI:10.1086/319981]
12. Gardiner SJ, Gavranich JB, Chang AB. Antibiotics for community-acquired lower respiratory tract infections secondary to Mycoplasma pneumoniae in children. Cochrane Database Syst Rev 2015;1-CD004875. [DOI:10.1002/14651858.CD004875.pub5]
13. Nour M, Trabelsi A, Maatouk N, Hammami M. Amplification of P1 and 16S rRNA genes by nested PCR for detection of Mycoplasma pneumoniae in paediatric patients. Pathologie Biologie 2005;53:9-14. [DOI:10.1016/j.patbio.2004.01.002]
14. Zhou Z, Li X, Chen X, Yao L, Pan C, Huang H, et al. Comparison of P1 and 16S rRNA genes for detection of Mycoplasma pneumoniae by nested PCR in adults in Zhejiang, China. J Infect Dev Ctries 2015;9:244-53. [DOI:10.3855/jidc.5149]
15. Bucholtz GA, Salzman SA, Bersalona FB, Boyle TR, Ejercito VS, Penno L, et al. PCR analysis of nasal polyps, chronic sinusitis, and hypertrophied turbinates for DNA encoding bacterial 16S rRNA. Am J Rhinol 2002;16:169-73. [DOI:10.1177/194589240201600309]
16. Thumerelle C, Deschildre A, Bouquillon C, Santos C, Sardet A, Scalbert M, et al. Role of viruses and atypical bacteria in exacerbations of asthma in hospitalized children: a prospective study in the Nord‐Pas de Calais region (France). Pediatr Pulmonol 2003;35:75-82. [DOI:10.1002/ppul.10191]
17. Han X, Li S, Lu S, Liu L, Li S, Zhang J. Amplification of 16S rDNA by nested PCR for measurement of Mycoplasma pneumoniae DNA over time: clinical application. J Med Microbiol 2012;61:426-30. [DOI:10.1099/jmm.0.030098-0]
18. Alp E, Voss A. Ventilator associated pneumonia and infection control. Ann Clin Microbiol Antimicrob 2006;5:7. [DOI:10.1186/1476-0711-5-7]
19. Akter S, Khatun R, Shamsuzzaman S. Molecular detection of atypical microorganisms in patients with ventilator associated pneumonia. Ibrahim Med Coll J 2015;9:22-5. [DOI:10.3329/imcj.v9i1.27636]
20. Moosavian S, Pordeli H. Survey of respiratory and urogenital infections due to Mycoplasma in the hospitalized patients in Ahwaz Imam Khomeini Hospital. Journal of Kerman University of Medical Sciences 2003;10:185-192. [In Persian]
21. Nolan T, Gadsby N, Hellyer T, Templeton K, McMullan R, McKenna J, et al. Low-pathogenicity Mycoplasma spp. alter human monocyte and macrophage function and are highly prevalent among patients with ventilator-acquired pneumonia. Thorax 2016;71:594-600. [DOI:10.1136/thoraxjnl-2015-208050]
22. El Seify MY, Fouda EM, Ibrahim HM, Fathy MM, Al Husseiny Ahmed A, Khater WS, et al. Microbial etiology of community-acquired pneumonia among infants and children admitted to the pediatric hospital, Ain Shams University. Eur J Microbiol Immunol 2016; 6:206-214. [DOI:10.1556/1886.2016.00022]
23. Apfalter P, Stoiser B, Barousch W, Nehr M, Kramer L, Burgmann H. Community-acquired bacteria frequently detected by means of quantitative polymerase chain reaction in nosocomial early-onset ventilator-associated pneumonia. Crit Care Med 2005;33:1492-8. [DOI:10.1097/01.CCM.0000169879.97129.7B]
24. Yamazaki T, Kenri T. Epidemiology of Mycoplasma pneumoniae infections in Japan and therapeutic strategies for macrolide-resistant M. pneumoniae. Front Microbiol 2016;7:693. [DOI:10.3389/fmicb.2016.00693]
25. Muir MT, Cohn SM, Louden C, Kannan TR, Baseman JB. Novel toxin assays implicate Mycoplasma pneumoniae in prolonged ventilator course and hypoxemia. Chest 2011;139:305-10. [DOI:10.1378/chest.10-1222]
26. Izumikawa K. Clinical features of severe or fatal Mycoplasma pneumoniae pneumonia. Front Microbiol 2016;7:800. [DOI:10.3389/fmicb.2016.00800]
27. Kashyap S, Sarkar M. Mycoplasma pneumonia: Clinical features and management. Lung India 2010;27:75-85. [DOI:10.4103/0970-2113.63611]
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Mafi S, Sakhaee F, Zargar M, Vaziri F, Zarei M, Rahimi Jamnani F, et al . The prevalence of Mycoplasma pneumoniae infection in patients with ventilator-associated pneumonia hospitalized in intensive care unit. MEDICAL SCIENCES. 2019; 29 (4) :313-321
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Volume 29, Issue 4 ( winter 2019) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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