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:: Volume 29, Issue 4 ( winter 2019) ::
MEDICAL SCIENCES 2019, 29(4): 322-328 Back to browse issues page
Investigating the prevalence of resistance genes in Listeria monocytogenes isolated from food and clinical samples
Ali Shivaee1 , Shahla Shahbazi2 , Afsaneh Gholami3 , Parham Kianoush pour4 , Faramarz Masjedian Jazi * 5
1- MSc in Medical Microbiology, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Ph.D Candidate, Department of Bacteriology, Pasteur Institute of Iran, Teheran, Iran
3- MSc in Microbiology, Department of Biology, Alborz Branch, Islamic Azad University, Karaj, Iran
4- Medical Microbiology Research Center, Qazvin University of Medical Science, Qazvin, Iran
5- Assistant Professor in Medical Microbiology, Department of Microbiology, School of Medicine, Iran University of , Fmasjedian@yahoo.com
Abstract:   (215 Views)
Background: Listeriosis can be fatal for vulnerable groups of society. The disease has been widespread in recent years due to the large consumption of dairy and meat products. There is little information about the susceptibility of antibiotics and the pattern of Listeria monostigenesis gene resistance in Iranian society. Accordingly, the present study was conducted to investigate the antibiotic susceptibility and genetic resistance pattern of Listeria monosteogenesis strains isolated from different clinical and environmental sources.
Materials and methods: In this study, 55 isolates were tested for antibiotic susceptibility by disk diffusion in agar and genetic pattern by polymerase chain reaction (PCR).
Results: 91% and 83% of the strains were resistant to streptomycin and Trimethoprim/sulfamethoxazole respectively. The result of PCR of antibiotic resistance genes showed that the prevalence of ermA, ermB, strA, tetA, tetS and ermC genes in isolates of Listeria monocytogenes was 50.90% (28/55), 21.81% (12/55), 89.9% (49/55), 0% (0/55), 21.81% (12/55) and 0% (0/55), respectively.
Conclusion: Due to the presence of 1/2a and 1/2c serotypes in isolated isolates and the presence of marker virulence genes in these strains, these isolates have potential for biological risks and listeriosis disease. Existence of this genetic pattern and resistance pattern can be partly due to the use of antibiotics during the production of dairy products. Regarding results of this study, the manner and rate of using animal antibiotics can be managed.
Keywords: Listeriosis, Community vulnerability groups, Listeria monocytogenesis, Antibiotic resistance, PCR.
Full-Text [PDF 338 kb]   (61 Downloads)    
Semi-pilot: Quazi-Experimental | Subject: Microbiology
Received: 2019/01/9 | Accepted: 2019/04/7 | Published: 2019/12/28
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Shivaee A, Shahbazi S, Gholami A, Kianoush pour P, Masjedian Jazi F. Investigating the prevalence of resistance genes in Listeria monocytogenes isolated from food and clinical samples. MEDICAL SCIENCES. 2019; 29 (4) :322-328
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Volume 29, Issue 4 ( winter 2019) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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