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The effect of curcumin on altered expression of BAX and BCL2 genes following cholestasis in the frontal region of male rats
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Shabnam Akbari1   , Mohammad Nasehi  2, Mehrdad Hashemi3 |
1- MSc,Department of Genetics,Tehran Medical Sciences,Islamic Azad University,Tehran,Iran
2- Associate Professor,Department of Physiology,Institute for Cognitive Science Studies(ICSS),Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , Nasehi@iricss.org
3- Professor,Department of Genetics,Tehran Medical Sciences, Islamic Azad University, Tehran, Iran |
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Abstract: (2192 Views) |
Background: Cholestasis occurs as a result of malfunction or secretion of bile; and complications of this disease include apoptosis (planned death). Curcumin is an effective extract of turmeric rhizome that has anti-apoptosis properties. Thus in this study, we investigated the expression of BAX and BCL2 genes following cholestasis and curcumin in the frontal region of male rats.
Materials and methods: This study was performed on 16 male rats weighing 220-240 gram in which they were divided into 4 groups, including control group, sham-curcumin, BDL group and BDL-curcumin. Then peripheral region of rats was collected and after extraction of RNA and cDNA synthesis, expression of genes in different groups was evaluated by Real Time PCR technique.
Results: BCL2 gene expression decreased in the BDL group compared to the control group(p<0.05), and BAX gene expression increased in the BDL group compared to the control group as a result,the cell progresses to apoptosis. However,with the injection of curcumin,the expression of BCL2 gene in the BDL-Curcumin group increased compared the BDL group and BAX gene expression decreased in the BDL-Curcumin compared to the BDL group.
Conclusion: Curcumin can increase the expression of BCL2 gene and decrease the expression of BAX gene in the frontal region,reduce the negative effects of cholestasis including apoptosis. |
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| Keywords: Cholestasis, Bax, Bcl2, Curcumin, Frontal |
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Full-Text [PDF 497 kb]
(1753 Downloads)
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Semi-pilot: Basic |
Subject:
Brain and Nerves System
Received: 2020/06/16 | Accepted: 2021/01/10 | Published: 2021/06/22
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