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The effect of aerobic training and resveratrol on some regulatory and executive factors of cardiomyocytes apoptosis in STZ-diabetic male rats
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Ali Mehri1    , Sedigheh Hosseinpour Delaware 2, Masoume Azizi3 , Mohammad Ali Azarbaijani4 , Parvin Farzangi5 |
1- PhD Candidate of Sport Physiology, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran
2- , delavar2009@iauksh.ac.ir
3- Department of Physical Education and Sport Sciences, Abadan Branch, Islamic Azad University, Abadan, Iran
4- Faculty of Physical Education and Sport Sciences, Islamic Azad University, Tehran Branch, Tehran, Iran
5- Department of Physiology, Islamic Azad University, Sari Branch, Sari, Iran |
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Abstract: (2775 Views) |
Background: The present study was conducted to investigate the effect of aerobic training with resveratrol supplementation on regulatory and executive factors of cardiomyocytes apoptosis in streptozocin diabetic male rats.
Materials and methods: In an experimental study, 50 male rats were randomly divided into 5 groups of 10 healthy controls, diabetics, resveratrol supplements, aerobic training and complementary resveratrol + aerobic training. Diabetes was induced by single injection of streptozotocin (50 ml/kg). Training was done 5 days per week for 8 weeks. They did exercise on the treadmill at 15m/min, 5 min and gradient of 15%. At the end of the course, all animals were completely and similarly treated and 48 hours after the last training session, anesthetized and sacrificed. After cardiac out and homogenization, the concentration of BAX, Bcl-2 and Caspase3 was measured individually.
Results: After induction of diabetes, the concentration of BAX and Caspase-3 significantly increased compared with healthy controls, while BCL-2 concentration decreased significantly. However, only exercise interaction and resveratrol had significant effect on BAX concentration and had no significant effect on Caspase-3 levels. Each exercise and resveratrol separately increased BCL-2 concentration, but their interactive effect was not significant.
Conclusion: Aerobic exercise and resveratrol have a beneficial effect on cardiac apoptosis in inducing diabetes, but their interaction is not significant in the process of cardiac apoptosis. Of course, aerobic exercise and resveratrol could be used as complementary therapies for myocardial protection against invasive apoptosis induced by diabetes. |
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| Keywords: Diabetes mellitus, Resveratrol, Aerobic exercise, BAX, Bcl-2, Caspase3. |
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Full-Text [PDF 349 kb]
(1342 Downloads)
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Semi-pilot: Controlled/Randomized clinical trial |
Subject:
Physiology
Received: 2019/01/13 | Accepted: 2019/07/2 | Published: 2020/04/15
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1. Anderson EJ, Rodriguez E, Anderson CA, Thayne K, Chitwood WR, Kypson AP. Increased propensity for cell death in diabetic human heart is mediated by mitochondrial-dependent pathways. Am J Physiol Heart Circ Physiol 2010;300:H118-24. [ DOI:10.1152/ajpheart.00932.2010] 2. Chao M, Zou D, Zhang Y, Chen Y, Wang M, Wu H, Ning G, Wang W. Improving insulin resistance with traditional Chinese medicine in type 2 diabetic patients. Endocrine 2009;36:268-74. [ DOI:10.1007/s12020-009-9222-y] 3. Mirfeizi M, Mehdizadeh Tourzani Z, Mirfeizi SZ, Asghari Jafarabadi M, Rezvani HR, Afzali M. Controlling type 2 diabetes mellitus with herbal medicines: a triple-blind randomized clinical trial of efficacy and safety. J Diabetes. 2016;8:647-56. [ DOI:10.1111/1753-0407.12342] 4. Sanders RA, Rauscher FM, Watkins III JB. Effects of quercetin on antioxidant defense in streptozotocin‐induced diabetic rats. J Biochem Mol Toxicol 2001;15:143-9. [ DOI:10.1002/jbt.11] 5. Sadi G, Kartal Dİ, Güray T. Regulation of glutathione S-transferase Mu with type 1 diabetes and its regulation with antioxidants. Turk J Biochem 2013; 38 ; 92-100. [ DOI:10.5505/tjb.2013.96720] 6. Ryter SW, Kim HP, Hoetzel A, Park JW, Nakahira K, Wang X, et al. Mechanisms of cell death in oxidative stress. Antioxid Redox Sign 2007;9:49-89. [ DOI:10.1089/ars.2007.9.49] 7. Sha J, Sui B, Su X, Meng Q, Zhang C. Alteration of oxidative stress and inflammatory cytokines induces apoptosis in diabetic nephropathy. Mol Med Rep 2017;16:7715-23. [ DOI:10.3892/mmr.2017.7522] 8. Maybaum S, Ilan M, Mogilevsky J, Tzivoni D. Improvement in ischemic parameters during repeated exercise testing: a possible model for myocardial preconditioning. Am J Cardiol 1996;78:1087-91. [ DOI:10.1016/S0002-9149(96)90057-0] 9. Shave R, Dawson E, Whyte G, George K, Gaze D, Collinson P. Altered cardiac function and minimal cardiac damage during prolonged exercise. Med Sci Sport Exer 2004;36:1098-103. [ DOI:10.1249/01.MSS.0000131958.18154.1E] 10. Dolinsky VW, Rogan KJ, Sung MM, Zordoky BN, Haykowsky MJ, Young Meet al. Both aerobic exercise and resveratrol supplementation attenuate doxorubicin-induced cardiac injury in mice. Am J Physiol Endoc M 2013;305:E243-53. [ DOI:10.1152/ajpendo.00044.2013] 11. Das S, Alagappan VK, Bagchi D, Sharma HS, Maulik N, Das DK. Coordinated induction of iNOS-VEGF-KDR-eNOS after resveratrol consumption: A potential mechanism for resveratrol preconditioning of the heart. Vas Pharmacol 2005;42:281-9. [ DOI:10.1016/j.vph.2005.02.013] 12. Lee PS, Chiou YS, Ho CT, Pan MH. Chemoprevention by resveratrol and pterostilbene: Targeting on epigenetic regulation. Biofactors 2018;44:26-35 [ DOI:10.1002/biof.1401] 13. Moeini Fard, M, Hedayati, M. Alloxan and streptozotocin, diabetes research tool. Applied Sport Physiology Research 2014;10(20):13-22. [In Persian] 14. Linden MA, Fletcher JA, Morris EM, Meers GM, Laughlin MH, Booth FW, et al. Treating NAFLD in OLETF rats with vigorous-intensity interval exercise training. Med Sci Sports Exerc 2015;47:556-58. [ DOI:10.1249/MSS.0000000000000430] 15. Shankar S, Singh G, Srivastava RK. Chemoprevention by resveratrol: molecular mechanisms and therapeutic potential. Front Biosci 2007;12:4839-54. [ DOI:10.2741/2432] 16. Torres M, Canal J, Perez C. Oxidative stress in normal and diabetic rats. Physiol Res 1999;48:203-208. 17. Pérez-Gallardo RV, Noriega-Cisneros R, Esquivel-Gutiérrez E, Calderón-Cortés E, Cortés-Rojo C, Manzo-Avalos S, et al. Effects of diabetes on oxidative and nitrosative stress in kidney mitochondria from aged rats. J Bioenerg Biomembr 2014;46:511-8. [ DOI:10.1007/s10863-014-9594-4] 18. Dehghan F, Soori R, Gholami K, Abolmaesoomi M, Yusof A, Muniandy Set al. Purslane (Portulaca oleracea) seed consumption and aerobic training improves biomarkers associated with atherosclerosis in women with type 2 diabetes (T2D). Sci Rep 2016;6:37819. [ DOI:10.1038/srep37819] 19. Fadini GP, Iori E, Marescotti MC, de Kreutzenberg SV, Avogaro A. Insulin-induced glucose control improves HDL cholesterol levels but not reverse cholesterol transport in type 2 diabetic patients. Atherosclerosis 2014;235:415-17. [ DOI:10.1016/j.atherosclerosis.2014.05.942] 20. Buluc M, Ayaz M, Turan B, Demirel-Yilmaz E. Resveratrol-induced depression of the mechanical and electrical activities of the rat heart is reversed by glyburide: evidence for possible K ATP channels activation. Arch Pharm Res 2007;30:603-7. [ DOI:10.1007/BF02977655] 21. Smoliga JM, Baur JA, Hausenblas HA. Resveratrol and health-a comprehensive review of human clinical trials. Mol Nutr Food Res 2011;55:1129-41. [ DOI:10.1002/mnfr.201100143] 22. Arbab-Zadeh A, Dijk E, Prasad A, Fu Q, Torres P, Zhang R, et al. Effect of aging and physical activity on left ventricular compliance. Circulation 2004;110:1799-805. [ DOI:10.1161/01.CIR.0000142863.71285.74] 23. Ulakcsai Z, Bagaméry F, Vincze I, Szökő É, Tábi T. Protective effect of resveratrol against caspase 3 activation in primary mouse fibroblasts. Croat Med J 2015;56:78-84. [ DOI:10.3325/cmj.2015.56.78] 24. Guo R, Li W, Liu B, Li S, Zhang B, Xu Y. Resveratrol protects vascular smooth muscle cells against high glucose-induced oxidative stress and cell proliferation in vitro. Med Sci Monit Basic Res 2014;20:82. [ DOI:10.12659/MSMBR.890858] 25. Khan RS, Fonseca-Kelly Z, Callinan C, Zuo L, Sachdeva MM, Shindler KS. SIRT1 activating compounds reduce oxidative stress and prevent cell death in neuronal cells. Front Cell Neurosci 2012; 31;6:63. [ DOI:10.3389/fncel.2012.00063] 26. Knowler WC, Barrett-Connor E, Fowler SE, Hamman RF, Lachin JM, Walker EA, et al. Reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin. N Engl J Med 2002;346:393-403. [ DOI:10.1056/NEJMoa012512] 27. Moien-Afshari F, Ghosh S, Khazaei M, Kieffer TJ, Brownsey RW, Laher I. Exercise restores endothelial function independently of weight loss or hyperglycaemic status in db/db mice. Diabetologia. 2008;51:1327-37. [ DOI:10.1007/s00125-008-0996-x] 28. Ghosh S, Khazaei M, Moien-Afshari F, Ang LS, Granville DJ, Verchere CB, et al. Moderate exercise attenuates caspase-3 activity, oxidative stress, and inhibits progression of diabetic renal disease in db/db mice. Am J Physiol Renal Physiol 2009;296:F700-8. [ DOI:10.1152/ajprenal.90548.2008] 29. Hong J-H, Kim M-J, Park M-R, Kwag O-G, Lee I-S, Byun BH, et al. Effects of vitamin streptozotocin-induced diabetic rats. Clinica chimica acta on oxidative stress and membrane fluidity in brain. Am J Physiol Renal Physiol 2004; 340:107-15. [ DOI:10.1016/j.cccn.2003.10.003] 30. Timmers S, Konings E, Bilet L, Houtkooper RH, van de Weijer T, Goossens GHet al. Calorie restriction-like effects of 30 days of resveratrol supplementation on energy metabolism and metabolic profile in obese humans. Cell Metabol 2011;14:612-22. [ DOI:10.1016/j.cmet.2011.10.002] 31. Bo S, Ciccone G, Castiglione A, Gambino R, De Michieli F, Villois P, et al. Anti-inflammatory and antioxidant effects of resveratrol in healthy smokers a randomized, double-blind, placebo-controlled, cross-over trial. Curr Med Chem 2013;20:1323-31. [ DOI:10.2174/0929867311320100009] 32. Kwak HB. Effects of aging and exercise training on apoptosis in the heart. J Exerc Rehabil 2013;9:212. [ DOI:10.12965/jer.130002] |
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Mehri A, Hosseinpour Delaware S, Azizi M, Azarbaijani M A, Farzangi P. The effect of aerobic training and resveratrol on some regulatory and executive factors of cardiomyocytes apoptosis in STZ-diabetic male rats. MEDICAL SCIENCES 2020; 30 (1) :59-66 URL: http://tmuj.iautmu.ac.ir/article-1-1742-en.html
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