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The effects of chlorodiazepoxide on the Bax gene expression in CA1 pyramidal cells of the newborn rat hippocampus
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Amin Dinarvand1   , Mehrdad Hashemi  2, Rasool Dinarvand3 , Shabnam Movaseghi4 , Mojtaba Jafarinia5 |
1- MSc, Department of Genetics, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran
2- Professor, Department of Genetics, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mhashemi@iautmu.ac.ir
3- Professor, Department of Pharmacy, Faculty of Pharmacy, Tehran Medical Sciences University, Tehran, Iran
4- Associate professor, Department of Anatomy, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
5- Assistant professor, Department of Genetics, Islamic Azad University, Marvdasht Branch, Marvdasht, Iran |
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Abstract: (2710 Views) |
Background: Most drugs used by pregnant women can pass through the placenta and expose embryos and developing embryos to teratogenic effects. Due to the increased cellular damage of Bax gene expression, the effect of cholorodiazepoxide use during pregnancy on Bax gene expression in the hippocampus of neonatal rats was investigated.
Materials and methods: After confirmation of pregnancy in Wistar female rats, intraperitoneal injection of chlordiazepoxide was administered at a dose of 10 mg/ kg daily for 21 days in the experimental group. Two weeks after birth, the infant's brain was removed from the skull. After isolating the CA1 region of the neonatal hippocampus, the expression of the Bax proapoptotic gene was examined and compared with the control and carrier group (saline).
Results: The level of gene expression was analyzed and propoptotic Bax gene showed significant increase in experimental group compared to control group.
Conclusion: The results of this study indicated that the administration of chlorodiazepoxide during pregnancy can cause neuronal damage in the hippocampus of Wistar rats. |
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| Keywords: Gene expression, Chlorodiazepoxide, Rat, Hippocampus |
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Full-Text [PDF 350 kb]
(3887 Downloads)
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Semi-pilot: Experimental |
Subject:
Genetic
Received: 2019/02/16 | Accepted: 2019/04/7 | Published: 2020/06/28
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Dinarvand A, Hashemi M, Dinarvand R, Movaseghi S, Jafarinia M. The effects of chlorodiazepoxide on the Bax gene expression in CA1 pyramidal cells of the newborn rat hippocampus. MEDICAL SCIENCES 2020; 30 (2) :134-140 URL: http://tmuj.iautmu.ac.ir/article-1-1771-en.html
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