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:: Volume 32, Issue 3 (Fall 2022) ::
MEDICAL SCIENCES 2022, 32(3): 281-292 Back to browse issues page
The effect of short endurance training on the expression level of PINK-1, Parkin and PGC-1α in the heart of nicotine-sensitized rats
Amir Abbas Lashgari1, Mohammad Ali Azarbayjani 2, Maghsoud Peeri1, Mohammad Nasehi3
1- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , m_azarbayjani@iauctb.ac.ir
3- Cognitive and Neuroscience Research Center (CNRC), Amir-Almomenin Hospital, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Abstract:   (167 Views)
Background: Nicotine alters the expression of various genes in the heart. PINK-1(PTEN-induced kinase1) is the major regulator of cellular mitophagy. Moreover, Parkin is a protein that plays a key role in the process of ubiquitination. Also, PGC-1ὰ (peroxisome proliferator-activated receptor gamma coactivator 1-alpha) is the main regulator of mitochondrial biogenesis. On the other hand, exercise has many positive physiological effects on patients suffering from heart failure. In this study, we aimed to investigate the effect of short endurance training on the expression of parkin, PINK1 and PGC- 1ὰ genes in the heart of nicotine-sensitive rats.
Materials and methods: in this study, male Wistar rats weighing approximately 180 to 200 gr were used. The animals received nicotine at dose of 0.21 mg/kg intraperitoneally. Real time PCR technique was used to evaluate the expression of genes.
Results: The results showed that nicotine decreased the expression of PGC-1ὰ gene (p<0.05) and had no effect on other genes (p>0.05). Short-term endurance training slightly increased the expression of all genes that was not statistically significant.
Conclusion: It seems that short-term exercise can reduce the pro–apoptotic and stimulant effects of oxidative stress induced by nicotine. In addition, long-term exercise may potentially induce a significant positive effect on mitophagy-related genes.
Keywords: Nicotine, Exercise, Heart, Parkin, PINK-1, PGC-1α
Full-Text [PDF 811 kb]   (69 Downloads)    
Semi-pilot: Experimental | Subject: Physiology
Received: 2020/08/9 | Accepted: 2022/09/5 | Published: 2022/09/19
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Lashgari A A, Azarbayjani M A, Peeri M, Nasehi M. The effect of short endurance training on the expression level of PINK-1, Parkin and PGC-1α in the heart of nicotine-sensitized rats. MEDICAL SCIENCES 2022; 32 (3) :281-292
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فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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