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Effects of alcoholic extract of Alhagi maurorum on the expression level of hepatic Caspase-3 gene in male Wistar rats following the induction of diabetes mellitus
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Tahmoores Shahrivar1    , Mokhtar Mokhtari 2, Vally Alipour3 |
1- Ph.D Student , Department of biology, Faculty of Science, Islamic Azad University- Kazeroon branch, Kazeroon, Iran
2- Professor, Department of Biology, Faculty of Sciences, Islamic Azad University, Kazeroon Branch, Kazeroon, Iran , M.Mokhtari246@yahoo.com
3- Assistant Professor, Department of environmental health, school of public health, Hormozgan university of medical sciences and health services, Hormozgan, Iran |
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Abstract: (3024 Views) |
Background: Many diseases, including diabetes, are involve in the development of liver disorders through changes in the expression of genes such as Caspase-3. In the present study, the impact of alcoholic extract of Alhagi maurorum on the expression level of liver Caspase-3 gene was investigated in male Wistar rats following the induction of diabetes mellitus by streptozotocin.
Materials and methods: In this experimental study, 42 male Wistar rats were divided into 6 groups. The control group was left untreated, the diabetic control group received one dose of 70 mg/kg streptozotocin, the experimental groups 1 and 2 received 250 and 500 mg/kg Alhagi maurorum alcoholic extract peritoneally respectively, and the experimental groups 3 and 4 first received streptozotocin followed by 250 and 500 mg/kg extracts respectively. To investigate the expression level of the Caspase-3 gene, liver samples were obtained from all the groups, and were evaluated using real time PCR.
Results: The expression of Caspase-3 gene, against β actine, increased significantly in experimental groups compared to the control group, while diabetic groups received extract showed reduced gene expression compared to the streptozotocin group.
Conclusion: It appears that Alhagi maurorum extract is effective in reducing hepatic gene expression of Caspase-3, which increases after using streptozotocin. This effect is possibly due to the anti-oxidants contents, such as flavonoids and anthocyanins. |
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| Keywords: Alhagi maurorum, Streptozotocin, Caspase-3, β actine, Rat. |
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Full-Text [PDF 661 kb]
(1612 Downloads)
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Semi-pilot: Experimental |
Subject:
Animal Biology
Received: 2020/02/12 | Accepted: 2020/05/11 | Published: 2020/12/20
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