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:: Volume 32, Issue 1 (Spring 2022) ::
MEDICAL SCIENCES 2022, 32(1): 21-30 Back to browse issues page
Phytosynthesis of silver nanoparticles with the aqueous extract of portulaca oleracea and its antimicrobial and antioxidant effect against gram-positive and gram-negative bacteria
Niloofar Elyasi1 , Simin Nabizadeh 2, Zahra Shafiei3
1- MSc in Microbiology, Department of Microbiology, School of Science and Agriculture, Islamic Azad University, Roudehen Branch, Tehran, Iran
2- Faculty, PhD in Cellular-Developmental-Plant Biology, Department of Biology, School of Science and Agriculture, Islamic Azad University, Roudehen Branch, Tehran, Iran , Nabizadeh.simin@gmail.com
3- Assistant Professor, PhD in Microbial Biotechnology, Department of Biology, School of Science and Agriculture, Islamic Azad University, Roudehen Branch, Tehran, Iran
Abstract:   (1593 Views)
Background: Due to the importance of investigating nosocomial infections and drug resistance in bacteria causing these infections, the use of herbs can be very helpful in treatment. In the present study, the effect of silver nanoparticles obtained from portulaca oleracea extract on bacteria causing nosocomial infections and effective parameters in the synthesis of these nanoparticles were investigated.
Materials and methods: First, an aqueous extract of portulaca oleracea was added to a 1 mM silver nitrate solution. The extract was used as a reducing and stabilizing agent for nanoparticles. The effect of effective parameters for the optimization of silver nanoparticles was evaluated by ultraviolet-visible spectroscopy and transmission electron microscopy. The antioxidant and antibacterial effect of the synthesized nanoparticles was determined by studying the activity of catalase, MIC, MBC, disk, and well method.
Results: The results showed the effect of alkaline pH as well as silver salt concentration on the quality of synthesized nanoparticles. Also, the results of the antimicrobial test showed that gram-negative bacteria revealed significant sensitivity to synthetic nanoparticles and Staphylococcus aureus showed the highest resistance to nanoparticles. Antioxidant properties were also observed against gram-negative strains.
Conclusion: The results showed that silver nanoparticles synthesized with portulaca oleracea had antibacterial and antioxidant properties. Therefore, photosynthesis of nanoparticles can be a good option for the treatment of infectious diseases caused by gram-negative bacteria.
Keywords: Phytosynthesis, Silver nanoparticles, Portulaca oleracea, Antimicrobial activity, Antioxidant activity.
Full-Text [PDF 487 kb]   (759 Downloads)    
Semi-pilot: Experimental | Subject: Microbiology
Received: 2021/07/6 | Accepted: 2021/10/25 | Published: 2022/03/21
1. رفرنس های متنی مثل خروجی کراس رف را در اینجا وارد کرده و تایید کنید 1. Rajabpour M, Arabestani mR, Yousefi mashof R, Alikhani MY. MIC determination of Pseudomonas aeruginosa strains were isolated from clinical specimens of patients admitted to educational hospitals in Hamedan (90-91). Iran J Med Microbiol 2013;7:18-25.
2. Zhou Y-X, Xin H-L, Rahman K, Wang S-J, Peng C, Zhang H. Portulaca oleracea L.: a review of phytochemistry and pharmacological effects. Biomed Res Int 2015;2015:925631. [DOI:10.1155/2015/925631]
3. Hashemi B, Taghiloo S, Allahmoradi E, Karami M, Rahdar HA. Assessment of antibacterial effect of hydro-alcoholic extract of Portulaca oleracea on the human pathogen bacteria. Journal of Sabzevar University of Medical Sciences 2018;25:303-8. [In Persian] 4. Safaei M. Investigation of the use of nanoparticles in the treatment of cancer and their mechanisms of action. National Conference on Nanotechnology Development; Tehran, Iran; 2017. 5. Naserian F, Heshmati F, Mehdizadeh Omrani M, Salarian R. An overview of nanoparticles and their application to drug delivery in cancer: review article. Tehran Univ Med J 2018;76:221-30. [In Persian] 6. Dousti B, Nabipour F, Hajiamraei A. Green Synthesis of Silver Nanoparticle Using Aqueous Extract of Fumaria Parviflora and Study of its Antibacterial and Antioxidant Properties. RJMS 2019;26:105-17. [In Persian] 7. Karimi J, Mohsenzadeh S. Plant synthesis of silver nanoparticles by Achillea wilhelmsii Pharmaceutical plant. RJMS 2013;20:64-9. 8. Calderón-Jiménez B, Johnson ME, Montoro Bustos AR, Murphy KE, Winchester MR, Vega Baudrit JR. Silver Nanoparticles: Technological Advances, Societal Impacts, and Metrological Challenges. Front Chem 2017;5:6. 9. Gholami A, Arabestani M R, Ahmadi M. Evaluation of antibacterial activity of aqueous and methanol extracts of Allium Jesdianum plant on a number of pathogenic bacteria resistant to antibiotics. PSJ 2016; 14:18-26. [In Persian] https://doi.org/10.21859/psj-140418 10. Goudarzi GH, Satari M, Aslanzadeh M, Goudarzi M, Bigdeli M. Effects of sub-inhibitory Concentrations OF German Chamomile (Matricaria Recotita) Extracts on The Activity of Catalase Enzyme of S. aureus. YAFTEH 2006;7: 49-55. [In Persian] 11. Mollania N, Gharibnia F, Rostami R, Dizaj T, Kheyrabadi M. Study on the antibacterial effects of silver nanoparticles produced by α-amylase enzyme. J Sabzevar Univ Medi Sci 2016;23:214-21. 12. Farrokhi Z, Kanvisi M, Ayati A. Silver Nanoparticles: A Survey on Chemical and Biological Synthesis Methods and their Antibacterial Behavior. J Biosaf 2018;1141-58. 13. Kakaei K, Ghadimi G. Synthesis of silver nanoparticles based on reduced graphene oxide as anelectrocatalyst for cathod side of fuel cells. J Appl Chem 2019;14:51-64. [DOI:10.3389/fchem.2017.00006]
4. Ghaderi RS, Kazemi M, Soleimanpour S. Nanoparticles are More Successful Competitor than Antibiotics in Treating Bacterial Infections: A Review of the Literature. IJMM 2021;15:18-45. [In Persian] [DOI:10.30699/ijmm.15.1.18]
5. Ghandehari S, Homayounitabrizi M, Ardalan P. Antioxidant And Cytotoxic Properties of Green Synthesized Silver Nanoparticles from Rubia tinctorum. JIUMS 2017:26:57-67. [In Persian]
6. Shali R, Neamati A, Ardalan P. Investigating the Cytotoxic Effect and Antioxidant Properties of Green Synthesized Silver Nanoparticles from the Root of Persicaria Bistorta on Human Liver Cancer Cell Line (Hep G2). IJMM 2018; 26:133-142. [In Persian] [DOI:10.29252/sjimu.26.6.133]
7. Mohtashami M, Sepehriseresht S, Asli E, Boroumand MA, Ghasemi A. Synthesis of silver nanoparticles through chemical reduction and biosynthesis methods and evaluation of their antibacterial effects. RJMS 2012;19:65-74. [In Persian]
8. Rajan R, Chandran K, Harper SL, Yun S-I, Kalaichelvan PT. Plant extract synthesized silver nanoparticles: an ongoing source of novel biocompatible materials. Ind Crops Prod 2015;70:356-73. . [DOI:10.1016/j.indcrop.2015.03.015]
9. Nikparast Y, Ghorbani R, Ahmadzadeh H, Asadi G. Green Synthesis of Silver Nanoparticles Using Amarantus retroflexus and Its Antibacterial Effect. J Plant Prot Res 2018;32:139-46. [In Persian]
10. Azizian Shermeh O, Valizadeh M, Valizadeh J, Taherizadeh M, Beigomi M. Phytochemical investigation and phytosynthesis of silver nanoparticles using aqueous extract of Capparis spinosa L. Modares J Biotech 2017;8:80-90.
11. Khatami M, Azizi Z, Pourseyedi S, Najarion O. Antibacterial effect of silver nanoparticles synthesized by green method against the standard strains Escherichia coli k12 and Escherichia coli 25922. Gorgan J Univ Med Sci 2016;17:119-24. [In Persian]
12. Shams S, Pourseyedi S. Green synthesis of silver nanoparticles in Melia azedarach fruit extract and screening its antimicrobial activity. Plant Prod Sci 2016;38:55-63.
13. Etemadi M, Mohebbi-Kalhori D, Shermeh OA, Qasemi A. Phytosynthesis of Silver Nanoparticles using aqueous extract of Camellia sinensis L. and study of their antibacterial activities. Fasa J Univ Med Sci 2017;7:39-52. [In Persian]
14. Shaik MR, Khan M, Kuniyil M, Al-Warthan A, Alkhathlan HZ, Siddiqui MRH, et al. Plant-extract-assisted green synthesis of silver nanoparticles using Origanum vulgare L. extract and their microbicidal activities. Sustainability 2018;10:913. [DOI:10.3390/su10040913]
15. Dolatabadi S, Emrani S, Mehrafruz E, Zhiani R. Green synthesis and antibacterial effect of silver nanoparticles using Eucalyptus camaldulensis. Neyshabur J Univ Med Sci 2017;5:74-85. [In Persian]
16. Jain D, Daima HK, Kachhwaha S, Kothari S. Synthesis of plant-mediated silver nanoparticles using papaya fruit extract and evaluation of their anti microbial activities. Dig J Nanomater Biostructures 2009;4:557-63.
17. Guzman M, Dille J, Godet S. Synthesis and antibacterial activity of silver nanoparticles against gram-positive and gram-negative bacteria. Nanomedicine 2012;8:37-45. [DOI:10.1016/j.nano.2011.05.007]
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Elyasi N, Nabizadeh S, Shafiei Z. Phytosynthesis of silver nanoparticles with the aqueous extract of portulaca oleracea and its antimicrobial and antioxidant effect against gram-positive and gram-negative bacteria. MEDICAL SCIENCES 2022; 32 (1) :21-30
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Volume 32, Issue 1 (Spring 2022) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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