[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 32, Issue 4 (winter 2022) ::
MEDICAL SCIENCES 2022, 32(4): 368-378 Back to browse issues page
The effect of residual acetamiprid and dichlorvos in greenhouse cucumber on liver function and testicular germ cells on laboratory mice
Hamid Salehi Mishani1 , Alireza Jalalizand 2, Mehrdad Modaresi3
1- Department of Plant protection, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
2- Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran , arjalalizand@gmail.com
3- Department of Animal Science, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran
Abstract:   (895 Views)
Background: Pesticides are widely used around the world, especially in underdeveloped and developing countries. However, the effect of residual amounts of these compounds on the physiological processes of the body has always been discussed.
Materials and methods: In this experimental study, 27cucumber plants were sprayed with a concentration equal to twofold recommended dose (acetamiprid (50 g/l) and dichlorvos (4 ml/l)). After 24 hours, the residual amount was obtained and to evaluate the effect of these residues, 20 mice were divided into 4 groups. The control group and groups 1, 2 and 3 that received dichlorvos,  acetamiprid, and combination of two pesticides respectively in drinking water.
Results: The residual pesticide in cucumber was obtained for acetamiprid 1.5 mg/kg and for dichlorvos 0.5 mg/kg. Their effect indicated a significant decrease in body weight and blood albumin levels (p<0.01) and an increase in liver enzymes (aspartate transaminase and alanine transaminase) and had changes in the number of testicular germ cells (p<0.05).
Conclusion: Liver disorders and reduced reproductive potential in male mice can be attributed to the addition of pesticides to their drinking water. Monitoring programs to evaluate the presence of residual pesticides in food should be done continuously and special attention should be paid to the management of consumption of these pesticides.
 
Keywords: Crops, Chemical pesticides, Liver, Germ cells, Laboratory mice
Full-Text [PDF 380 kb]   (845 Downloads)    
Semi-pilot: Experimental | Subject: Animal Biology
Received: 2022/05/22 | Accepted: 2022/08/28 | Published: 2022/12/31
References
1. United Nations. Mobilizing Countries to Beat Back 'Voracious' Desert Locust Threat in East Africa. Available from: https://news.un.org/en/audio/2020/02/1057121. 2. Sarkara S, Dias J, Keeley J, Mohring N, Jansen K. The use of pesticides in developing countries and their impact on health and the right to food. Belgium: Policy Department for External Relations Directorate General for External Policies of the :union:; 2021. 3. Eddleston M. Poisoning by pesticides. Medicine 2020; 48: 214-217. 4. Jia ZQ, Zhang YC, Huang QT, Jones AK, Han ZJ, Zhao CQ. Acute toxicity, bioconcentration, elimination, action mode and detoxification metabolism of broflanilide in zebrafish, Danio rerio. J Hazard Mater 2020;394:122521. https://doi.org/10.1016/j.jhazmat.2020.122521 5. Bertero A, Chiari M, Vitale N, Zanoni M, Faggionato E, Biancardi A, Caloni F. Types of pesticides involved in domestic and wild animal poisoning in Italy. Sci Total Environ 2020;707:136129. https://doi.org/10.1016/j.scitotenv.2019.136129 6. Owens K, Feldman J, Kepner J. Wide Range of Diseases Linked to Pesticides, Database supports policy shift from risk to alternatives assessment. Pestic and You 2010;30: 13-21. 7. El Nemr A, Ed. Impact, Monitoring and Management of Environmental Pollution. Hauppauge, New York: Nova Science Publishers, Inc; 2011. p. 638. 8. Sjerps RMA, Kooij PJF, van Loon A, Van Wezel AP. Occurrence of pesticides in Dutch drinking water. Chemosphere 2019; 235:510-518. https://doi.org/10.1016/j.chemosphere.2019.06.207 9. Warra AA, Prasad MNV. African perspective of chemical usage in agriculture and horticulture-their impact on human health and environment. Prasad MNV, Ed. Agrochemicals Detection, Treatment and Remediation Pesticides and Chemical Fertilizers. New York: Elsevier; 2020. P.401-436. https://doi.org/10.1016/B978-0-08-103017-2.00016-7 10. Juraske R, Mutel CL, Stoessel F, Hellweg S. Life cycle human toxicity assessment of pesticides: comparing fruit and vegetable diets in Switzerland and the United States. Chemosphere 2009;77:939-45. https://doi.org/10.1016/j.chemosphere.2009.08.006 11. Zikankuba VL, Mwanyika G, Ntwenya JE, James A. Pesticide Regulations and their Malpractice Implications on Food and Environment Safety. Cogent Food & Agriculture 2019;5:1-15. https://doi.org/10.1080/23311932.2019.1601544 12. Environmental Working Group (EWG). EWG's 2021 Shopper's Guide to Pesticides in Produce™. Over 90 Percent of Non-Organic Citrus Fruits Contain Fungicides Linked to Cancer and Hormone Disruption. Aqvailable from: https://www.ewg.org/news-insights/news-release/out-now-ewgs-2021-shoppers-guide-pesticides-producetm. 13. Buszewski B, Bukowska M, Ligor M, Staneczko-Baranowska I. A holistic study of neonicotinoids neuroactive insecticides-properties, applications, occurrence, and analysis. Environ Sci Pollut Res Int 2019;26:34723-34740. https://doi.org/10.1007/s11356-019-06114-w 14. Nguyen TT, Rosello C, Bélanger R, Ratti C. Fate of Residual Pesticides in Fruit and Vegetable Waste (FVW) Processing. Foods 2020 ;9:1468. https://doi.org/10.3390/foods9101468 15. McPherson R, Pincus M, Eds. Henry's clinical diagnosis and management by laboratory methods. 23rd Ed. New York; Elsevier; 2017. 16. Mostofi FK, Spaander P, Gingor K. Cousensus on pathological clasihication of testicular germ cell tumors. Prog Clin Biol 1990: 357,267-276. 18. Kouchesfahani HM, Parivar K, Eds. General Histological Embryological and Zoological Microtechniques. First Ed. Tehran: Al-Hosein Co: 2001. pp: 48-49. 19. Thorburn C. The Rise and Demise of Integrated Pest Management in Rice in Indonesia. Insects 2015 17;6:381-408. https://doi.org/10.3390/insects6020381 20. Dong J, Gong X, Zhang L. Wang H. Determination of imidacloprid tebufenozide、 avennectins and hexythiazox in vegetables by QuEChERS-HPLC. Chinese Journal of Analysis Laboratory 2008:S481.8. 21. Yawar Latif STH, Sherazi M, Bhanger I. Assessment of pesticide residues in commonly used vegetables in Hyderabad, Pakistan. Ecotoxicol Environ Saf 2011;8:2299-2303. https://doi.org/10.1016/j.ecoenv.2011.07.030 22. Amrollahi H, Pazoki R, Imani S. Pesticide Multiresidue Analysis in Tomato and Cucumber Samples Collected from Fruit and Vegetable Markets in Tehran, Iran. Middle East J Rehabil Health Stud 2018;6::e64271. https://doi.org/10.5812/mejrh.64271 23. Galani JHY, Houbraken M, Wumbei A, Djeugap JF, Fotio D, Spanoghe P. Evaluation of 99 Pesticide Residues in Major Agricultural Products from the Western Highlands Zone of Cameroon Using QuEChERS Method Extraction and LC-MS/MS and GC-ECD Analyses. Foods 2018;7:184. https://doi.org/10.3390/foods7110184 24. Galani JHY, Houbraken M, Wumbei A, Djeugap JF, Fotio D, Spanoghe P. Evaluation of 99 Pesticide Residues in Major Agricultural Products from the Western Highlands Zone of Cameroon Using QuEChERS Method Extraction and LC-MS/MS and GC-ECD Analyses. Foods 2018;7:184. https://doi.org/10.3390/foods7110184 25. Ibrahim AR, Abdelbasset Ibrahim M. Malathion induced testicular toxicity and oxidative damage in male mice: the protective effect of curcumin. Egypt J Forensic Sci 2018;70:1-13. 26. Slimen S, Saloua EF, Najoua Gh. Oxidative stress and cytotoxic potential of anticholinesterase insecticide, Malathion in reproductive toxicology of male adolescent mice after acute exposure. Iran J Basic Med Sci 2014; 17:522-530. 27. Abdel-Tawab, H Mossa, Tarek, M., Heikal. Enayat Abdel Aziz Omara. Physiological and histopathological changes in the liver of male rats exposed to paracetamol and diazinon. Asian Pac J Trop Biomed 2012;1683-S1690. https://doi.org/10.1016/S2221-1691(12)60478-X 28. Sankhala LN, Tripathi SM, Bhavsar SK, Thaker AM, Sharma P. Hematological and immunological changes due to shortterm oral administration of acephate. Toxicol Int 2012; 19:162-6. https://doi.org/10.4103/0971-6580.97217 29. Mukherji A, Bailey SM, Staels B, Baumert TF. The circadian clock and liver function in health and disease. J Hepatol 2019; 71:200-211. https://doi.org/10.1016/j.jhep.2019.03.020 30. Liu H. Hussain SH. Ali D,Al Omar S. Shalik U. Alghamdi H and Maddu N. Induced alteration of rat erythrocyte membrane with effect of pyrethroid based compounds. Saudi J Biol Sci 2020:273669-3675. https://doi.org/10.1016/j.sjbs.2020.08.011 31. Assaraj QSH, Alattar HA, Farid AS, Fararah KM. Influence of Lactoferrin on immune response in rats intoxicated by diazinon. Benha Veterinary Medical Journal 2018; 34; 169-181. https://doi.org/10.21608/bvmj.2018.29426 32. Roche M, Rondeau P, Singh NR, Tarnus E, Bourdon E. The antioxidant properties of serum albumin. FEBS Lett 2008;582:1783-7. https://doi.org/10.1016/j.febslet.2008.04.057 33. Ambali SF, Ayo JO, Ojo SA, Esievo KA. Ameliorative effect of vitamin C on chronic chlorpyrifos-induced erythrocyte osmotic fragility in Wistar rats. Hum Exp Toxicol 2011;30:19-24. https://doi.org/10.1177/0960327110368415 34. Goel A, Danni V, Dhawan DK. Role of zinc in mitigating the toxic effects of chlorpyrifos on hematological alterations and electron microscopic observations in rat blood. BioMetals 2006;19: 483-492. https://doi.org/10.1007/s10534-005-5148-x 35. Rabideau CL. Pesticide mixtures induce immunotoxicity: potentiation of apoptosis and oxidative stress. MSc Thesis. Blacksburg, Virginia: Virginia Polytechnic Institute and State University; 2001. 36. Davis LM, Pei Z, Trush MA, Cheskin LJ, Contoreggi C, McCullough K. Bromocriptine reduces steatosis in obese rodent modelsd. J Hepatol 2006; 45: 439-44. https://doi.org/10.1016/j.jhep.2006.03.019 37. Elgaml ASH, Hassan W, Abdelaziz S, Hashish E. Parkinsonia aculeata L. aqueous extract alleviated the hepatotoxicity induced by acetaminophen in albino rats. Compar Clin Pathol 2020;29:1-7. https://doi.org/10.1007/s00580-020-03102-2 38. Abdelazeim SA, Shehata NI, Aly HF, Shams SGE. Amelioration of oxidative stress-mediated apoptosis in copper oxide nanoparticles-induced liver injury in rats by potent antioxidants. Sci Rep 2020;10:10812. https://doi.org/10.1038/s41598-020-67784-y 39. Kartheek RM, David M. Assessment of fipronil toxicity on wistar rats: A hepatotoxic perspective. Toxicol Rep 2018;5:448-456. https://doi.org/10.1016/j.toxrep.2018.02.019 40. Jaiswal K, Gupta V, Siddiqi N, Pandey R, Sharma B. Hepatoprotective Effect of Citrus limon Fruit Extract against Carbofuran Induced Toxicity in Wistar Rats. Chinese J Biol 2015;1- 10. https://doi.org/10.1155/2015/686071 41. Lasram MM, Lamine AJ, Dhouib IB, Bouzid K, Annabi A, Belhadjhmida N, et al. Antioxidant and anti-inflammatory effects of N-acetylcysteine against malathion-induced liver damages and immunotoxicity in rats. Life Sci 2014;107:50-8. https://doi.org/10.1016/j.lfs.2014.04.033 42. Ojezele, Matthew Obaineh, Abatan, Oluwole Matthew. Toxicological effects of chlorpyrifos and methidathion in young chickens. African J Biochem Res 2009;3: 048-051. 43. Awad ME, Abdel-Rahman MS, Hassan SA. Acrylamide toxicity in isolated rat hepatocytes. Toxicol In Vitro 1998;12:699-704. https://doi.org/10.1016/S0887-2333(98)00051-4 44. Ilavenil S, Al-Dhabi NA, Srigopalram S, Ock Kim Y, Agastian P, Baru R, Choi KC, Valan Arasu M. Acetaminophen Induced Hepatotoxicity in Wistar Rats--A Proteomic Approach. Molecules 2016;21:161. https://doi.org/10.3390/molecules21020161 45. Khan SM, Sobti RC, Kataria L. Pesticide-induced alteration in mice hepato-oxidative status and protective effects of black tea extract. Clin Chim Acta 2005;358:131-8. https://doi.org/10.1016/j.cccn.2005.02.015 46. Meyer SA, Kulkarni AP. Hepatotoxicity. In: Hodgson E, Smart RC, Eds. Introduction to Biochemical Toxicology. 3rd Ed. New York: Wiley and Sons; 2001. p: 487-490. 47. Mostofi FK, Spaander P, Gingor K. Cousensus on pathological clasihication of testicular germ cell tumors. Prog Clin Biol 1990;357:267-276. 48. Nikoosirjahromi M, Ranjbar R, Khaksarmahabadi M, Morovati H, Najafzadehvarzi H. Effects of Curcumin on Histologic Lesions of Testis Induced by Aflatoxin B1 in Rat Offspring before and after Puberty. Sci J Ilam Uni Med Sc. 2017;24: 41-53. [In Persian] https://doi.org/10.18869/acadpub.sjimu.24.6.41 49. Aslam F, Khan A, Khan MZ, Sharaf S, Gul ST, Saleemi MK. Toxico-pathologicalchange induced by p-NP in broiler chicks; their attenuation with vitamin E and selenium. Exp Toxicol Pathol 2010;62:441-50. https://doi.org/10.1016/j.etp.2009.06.004 50. Bal R, Türk G, Tuzcu M, Yılmaz Ö, Kuloğlu T, Baydaş G, et al. Effects of the neonicotinoid insecticide, clothianidin, on the reproductive organ system in adult male rats. Drug Chem Toxicol 2013;36:421-9. https://doi.org/10.3109/01480545.2013.776575 51. Arıcan EY, Gökçeoğlu Kayalı D, Ulus Karaca B, Boran T, Öztürk N, Okyar A, et al. Reproductive effects of subchronic exposure to acetamiprid in male rats. Sci Rep 2020;10:8985. https://doi.org/10.1038/s41598-020-65887-0 52. D'Occhio MJ, Hengstberger KJ, Johnston SD. Biology of sperm chromatin structure and relationship to male fertility and embryonic survival. Anim Reprod Sci 2007;101:1-17. https://doi.org/10.1016/j.anireprosci.2007.01.005 53. Recio-Vega R, Ocampo-Gómez G, Borja-Aburto VH, Moran-Martínez J, Cebrian-Garcia ME. Organophosphorus pesticide exposure decreases sperm quality: association between sperm parameters and urinary pesticide levels. J Appl Toxicol 2008;28:674-80. https://doi.org/10.1002/jat.1321 54. Joshi SC, Mathur R, Gulati N. Testicular toxicity of chlorpyrifos (an organophosphate pesticide) in albino rat. Toxicol Ind Health 2007;23:439-44. https://doi.org/10.1177/0748233707080908 55. Mnif W, Ibn Hadj Hassine A, Bouaziz A, Bartegi A, Thomas O, Roig B. Effect of endocrine disruptor pesticides: a review. Int j Environ Res Public Health 2011;8:2265-303. https://doi.org/10.3390/ijerph8062265 56. Mandal TK, Das NS. Testicular gametogenic and steroidogenic activities in chlorpyrifos insecticide-treated rats: a correlation study with testicular oxidative stress and role of antioxidant enzyme defence systems in Sprague-Dawley rats. Andrologia 2012;44:102-15. https://doi.org/10.1111/j.1439-0272.2010.01110.x [DOI:10.1016/j.mpmed.2019.12.019]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Salehi Mishani H, Jalalizand A, Modaresi M. The effect of residual acetamiprid and dichlorvos in greenhouse cucumber on liver function and testicular germ cells on laboratory mice. MEDICAL SCIENCES 2022; 32 (4) :368-378
URL: http://tmuj.iautmu.ac.ir/article-1-2008-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 32, Issue 4 (winter 2022) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.05 seconds with 37 queries by YEKTAWEB 4645