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Investigating the effect of intramyocardial injection of DNAzyme-containing nisosome of NF-κB gene on the level of cardiac tissue NF-κB protein expression in cardiac ischemia-reperfusion of male rats
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Seyede Bahar Hosseinian1    , Nahid Aboutaleb2 , Maryam Naseroleslami3 , Neda Mousavi Niri 4 |
1- Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Physiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran
3- Department of Cellular and Molecular Biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
4- Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mousaviniri@gmail.com |
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Abstract: (299 Views) |
Background: NF-κB factor is activated by ischemic damage and reperfusion and has a crucial role in transcription of several genes. Applying DNAzyme is one of the novel therapeutic means. Niosome is a new drug delivery system that is controllable ae used targeted. Applying DNAzyme-containing nanoniosome to diminish the expression of NF-κB protein after cardiac ischemia.
Materials and methods: In the current study, twenty-five male rats were assigned into 5 groups: 1- Control group, 2- Ischemic induced group, 3- Post ischemic DNAzyme receiving group, 4- Post ischemic DNAzyme-bearing nanoniosome receiving group, and 5- Post ischemic nanoniosome receiving group. After 72 hours, western blotting was performed on rats’ hearts to assess expression of NF-κB protein in treated groups.
Results: post ischemic treatment with DNAzyme-bearing nanoniosome led to considerably decrease of NF-κB expression compared to ischemic rats (P<0.05).
Conclusion: Results demonstrated the efficiency of DNAzyme-containing nanoniosome on NF-κB protein after cardiac ischemia. Therefore it is propounded as a curative method for cardiac ischemia |
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| Keywords: DNAzyme, NF-κB, cardiac ischemia |
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Full-Text [PDF 517 kb]
(160 Downloads)
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Semi-pilot: Experimental |
Subject:
Molecular Biology
Received: 2023/05/10 | Accepted: 2023/06/28 | Published: 2023/12/1
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| References |
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Hosseinian S B, Aboutaleb N, Naseroleslami M, Mousavi Niri N. Investigating the effect of intramyocardial injection of DNAzyme-containing nisosome of NF-κB gene on the level of cardiac tissue NF-κB protein expression in cardiac ischemia-reperfusion of male rats. MEDICAL SCIENCES 2023; 33 (4) :338-346 URL: http://tmuj.iautmu.ac.ir/article-1-2111-en.html
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