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:: Volume 34, Issue 2 (summer 2024) ::
MEDICAL SCIENCES 2024, 34(2): 146-154 Back to browse issues page
Phenotypic and genotypic investigation of the co-cultivation of intestinal probiotic lactobacilli on the amount of biofilm production in Shigella typhi
Moseib Gol Mohammadi1 , Mahnaz Mohammadi 2
1- Senior Expert, Department of Biotechnology, Tehran Medical Sciences, Islamic Azad University,Tehran, Iran
2- Assistant Professor, Department of Biology, Islamshahr Branch, Islamic Azad University, Islamshahr, Iran , mh_mohamadi@yahoo.com
Abstract:   (495 Views)
Background: Diarrhea is one of the most important causes of death in the world. Shigella is one of the causes of diarrheal diseases. Global studies have also shown that the prevalence of this bacterium as well as the resistance in Shigella are strongly increasing. Also, the resistance of Shigella species to third generation cephalosporins is increasing. The purpose of this study was to investigate the effect of Lactobacillus plantarum probiotics on the survival rate and also to investigate the expression of surface factors related to survival and bacterial biofilm.
Materials and methods: In this study, a sample of Shigella bacteria was prepared according to ATCC standard from Pasteur Institute of Iran and Lactobacillus plantarum isolated from dairy products was used. Samples were freshly cultured in culture medium. Then the effect of Lactobacillus plantarum on Shigella was co-cultured in 96-well plates in the presence of 1% glucose. After 24 hours RNA extraction and cDNA synthesis were performed and the survival and expression of the genes for the production of binding factors and bacterial biofilm were evaluated by Real Time technique.
Results: This study showed that the co-cultivation of Lactobacillus plantarum could significantly reduce the population of Shigella bacteria and also significantly reduced the expression of genes related to BSSS and OSPE 2 binding factors.
Conclusion: The results of this study showed that Lactobacillus plantarum could have an effect on the control and recovery process of Shigella bacteria.
 
Keywords: Lactobacillus plantarum, Shigella, Biofilm, Probiotic
Full-Text [PDF 781 kb]   (137 Downloads)    
Semi-pilot: Controlled/Randomized clinical trial | Subject: biotechnology
Received: 2023/08/1 | Accepted: 2023/11/5 | Published: 2024/06/30
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Gol Mohammadi M, Mohammadi M. Phenotypic and genotypic investigation of the co-cultivation of intestinal probiotic lactobacilli on the amount of biofilm production in Shigella typhi. MEDICAL SCIENCES 2024; 34 (2) :146-154
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Volume 34, Issue 2 (summer 2024) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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