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Investigating the application of graphene quantum dots doped with heteroatoms as a contrast agent in magnetic resonance imaging system
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Morteza Mollazadeh1    , Farshid Babapour Mofrad 2, Ashraf Fakhari3 , Ali Jamali Nazari4 , Tohid Mortazazadeh5 |
1- Ph.D. Student of Department of Medical Radiation Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Associate Professor, Department of Medical Radiation Engineering, Science and Research Branch, Islamic Azad University, Tehran, Iran , Babapour@srbiau.ac.ir
3- Assistant Professor, Department of Nuclear Medicine, Faculty of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran
4- Assistant Professor, Department of Engineering, Shahrood Branch, Islamic Azad University, Shahrood Branch, Shahrood, Iran
5- Assistant Professor, Department of Medical Physics, Faculty of Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran |
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Abstract: (165 Views) |
Background: Contrast agents used in MRI are mostly based on metallic elements such as gadolinium and are toxic.The present study aims to prepare a non-metallic and non-toxic nanoparticle based on quantum data carbon, which can be used as a non-metallic and non-toxic contrast agent in MRI.
Materials and methods: The cytotoxicity of nanoparticles (PN-GQDOTs) against MCF-7 and MCF-10A cells was investigated using the MTT method. The value of longitudinal relaxation r1 in the phantom model was calculated using MRI imaging with spin-echo protocol. The ability of nanoparticles to penetrate into cancer cells was determined using MRI imaging.
Results: Cytotoxicity assay results showed no significant decrease in cell viability with different concentrations of NPs for the incubation period of 24 hours. With MRI imaging in the phantom environment, r1=4.49 was obtained for the synthesized nanoparticle, which was a significant increase compared to the dotarem material (r1=2.54).The results of animal imaging showed the ability of nanoparticles to create a difference in the contrast of the images before and after the injection of the contrast agent.
Conclusion: The results exhibited low cytotoxicity of the PN-GQDOTs nanoparticles in the given concentration range,which is essential for MRI imaging and further biological in vitro and in vivo applications.According to the results of the animal study, these nanoparticles can be considered as a positive and non-toxic contrast agent, which minimizes the concern about the toxicity of this medical system due to the absence of metal compounds in the structure of this compound.
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| Keywords: Graphene quantum dots, MRI, Cytotoxicity |
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Full-Text [PDF 976 kb]
(161 Downloads)
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Semi-pilot: Experimental |
Subject:
Medical Physics
Received: 2023/10/14 | Accepted: 2024/01/20 | Published: 2024/10/1
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Mollazadeh M, Babapour Mofrad F, Fakhari A, Jamali Nazari A, Mortazazadeh T. Investigating the application of graphene quantum dots doped with heteroatoms as a contrast agent in magnetic resonance imaging system. MEDICAL SCIENCES 2024; 34 (3) :245-254 URL: http://tmuj.iautmu.ac.ir/article-1-2179-en.html
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