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Investigating the morphology, morphometry and behavioral analysis of the effect of demyelination on the hippocampus of rats in the model of multiple sclerosis induced by cuprizone
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Mehdi Rashnavadi1    , Nematolla Shakarami2 , Salman Soltani3 , Hajar Azizian4 , Aref Nooraei 5 |
1- Assistant Professor, Department of Veterinary Laboratory Sciences, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
2- PhD Student in Histology, Faculty of Veterinary Medicine, Tehran University, Tehran, Iran
3- Assistant Professor, Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
4- PhD in Histology, Central Laboratory, Ilam University, Ilam, Iran
5- Assistant Professor, Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran , a.nooraei@ilam.ac.ir |
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Abstract: (352 Views) |
Background: Multiple sclerosis is an autoimmune and inflammatory disease of the central nervous system that causes the degeneration of neurons in the hippocampus, cognitive disorders, memory disorders, and imbalance. This disease is three times more prevalent in women than men. The present study compared the destruction of astrocytes in the hippocampus and the subsequent functional disorders in both males and females without hormonal intervention.
Materials and methods: 20 adult rats were used in this study. The animals were randomly divided into 4 groups: male control, female control, male MS and female MS. In the MS groups, MS was induced by 0.02% cuprizone orally. Then they were subjected to a behavioral study for 6 weeks, and at the end of the experiment, a tissue sample from the hippocampus was studied. The data were analyzed by SPSS version 16 software. The data were presented as mean ± standard error. Analysis was done by one-way analysis of variance.
Results: There were weight loss, reduced brain anatomical parameters, neuron destruction and imbalance in both male and female sexes compared to other groups, and the severity of neuron destruction was more in the hippocampus of males than in females.
Conclusion: Although MS is more common in women than men, the severity of the destruction of brain structures and nerve cells is greater in men.
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| Keywords: Morphology, Morphometry, Behavioral analysis, Hippocampus, Multiple sclerosis |
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Full-Text [PDF 1316 kb]
(116 Downloads)
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Semi-pilot: Experimental |
Subject:
Brain and Nerves System
Received: 2024/02/3 | Accepted: 2024/04/14 | Published: 2024/11/30
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1. Nooraei A, Khazaeel K, Darvishi M, Ghotbeddin Z, Basir Z. Dimorphic evaluation of hippocampal changes in rat model of demyelination: A comparative functional, morphometric, and histological study. Brain Behav 2022;12:e32723. [ DOI:10.1002/brb3.2723] 2. Bauer C, Dyrby TB, Sellebjerg F, Madsen KS, Svolgaard O, Blinkenberg M, et al. Motor fatigue is associated with asymmetric connectivity properties of the corticospinal tract in multiple sclerosis. NeuroImage Clin 2020;28:102393. [ DOI:10.1016/j.nicl.2020.102393] 3. Avigan PD, Cammack K, Shapiro ML. Flexible spatial learning requires both the dorsal and ventral hippocampus and their functional interactions with the prefrontal cortex. Hippocampus 2020;30:733-44. [ DOI:10.1002/hipo.23198] 4. Magyari M, Sorensen PS. The changing course of multiple sclerosis: rising incidence, change in geographic distribution, disease course, and prognosis. Curr Opin Neurol 2019;32:320-6. [ DOI:10.1097/WCO.0000000000000695] 5. Guo S, Tian Z, Wu QW, King-Jones K, Liu W, Zhu F, et al. Steroid hormone ecdysone deficiency stimulates preparation for photoperiodic reproductive diapause. PLoS Genet 2021;17:e1009352. [ DOI:10.1371/journal.pgen.1009352] 6. Tanriverdi F, Silveira LF, MacColl GS, Bouloux PM. The hypothalamic-pituitary-gonadal axis: immune function and autoimmunity. J Endocrinol 2003;176:293-304. [ DOI:10.1677/joe.0.1760293] 7. Borrás C, Sastre J, García-Sala D, Lloret A, Pallardó FV, Viña J. Mitochondria from females exhibit higher antioxidant gene expression and lower oxidative damage than males. Free Radic Biol Med 2003;34:546-52. [ DOI:10.1016/S0891-5849(02)01356-4] 8. Kipp M, Clarner T, Dang J, Copray S, Beyer C. The cuprizone animal model: new insights into an old story. Acta Neuropathol 2009;118:723-36. [ DOI:10.1007/s00401-009-0591-3] 9. Jhelum P, Santos-Nogueira E, Teo W, Haumont A, Lenoël I, Stys PK, et al. Ferroptosis mediates cuprizone-induced loss of oligodendrocytes and demyelination. J Neuroscience 2020;40:9327-41. [ DOI:10.1523/JNEUROSCI.1749-20.2020] 10. Buscarinu MC, Reniè R, Morena E, Romano C, Bellucci G, Marrone A, et al. Late-onset MS: disease course and safety-efficacy of DMTS. Front Neurol 2022;13:829331. [ DOI:10.3389/fneur.2022.829331] 11. Zirngibl M, Assinck P, Sizov A, Caprariello AV, Plemel JR. Oligodendrocyte death and myelin loss in the cuprizone model: an updated overview of the intrinsic and extrinsic causes of cuprizone demyelination. Mol Neurodegener 2022;17:1-28. [ DOI:10.1186/s13024-022-00538-8] 12. Boltze J, Kowalski I, Geiger K, Reich D, Gunther A, Buhrle C, et al. Experimental treatment of stroke in spontaneously hypertensive rats by CD34+ and CD34-cord blood cells. GMS German Med Sci 2005;3. 13. Ziehn MO, Avedisian AA, Dervin SM, Umeda EA, O'Dell TJ, Voskuhl RR. Therapeutic testosterone administration preserves excitatory synaptic transmission in the hippocampus during autoimmune demyelinating disease. J Neurosci 2012;32:12312-24. [ DOI:10.1523/JNEUROSCI.2796-12.2012] 14. Khan Z, Gupta GD, Mehan S. Cellular and molecular evidence of multiple sclerosis diagnosis and treatment challenges. J Clin Med 2023;12:4274. [ DOI:10.3390/jcm12134274] 15. Ma K, Xiong N, Shen Y, Han C, Liu L, Zhang G, et al. Weight loss and malnutrition in patients with Parkinson's disease: current knowledge and future prospects. Front Aging Neurosci 2018;10:1. [ DOI:10.3389/fnagi.2018.00001] 16. Mojaverrostami S, Pasbakhsh P, Madadi S, Nekoonam S, Zarini D, Noori L, et al. Calorie restriction promotes remyelination in a Cuprizone-Induced demyelination mouse model of multiple sclerosis. Metab Brain Dis 2020;35:1211-24. [ DOI:10.1007/s11011-020-00597-0] 17. Bai M, Wang Y, Han R, Xu L, Huang M, Zhao J, et al. Intermittent caloric restriction with a modified fasting-mimicking diet ameliorates autoimmunity and promotes recovery in a mouse model of multiple sclerosis. J Nutr Biochem 2021;87:108493. [ DOI:10.1016/j.jnutbio.2020.108493] 18. Miller DH, Barkhof F, Frank JA, Parker GJ, Thompson AJ. Measurement of atrophy in multiple sclerosis: pathological basis, methodological aspects and clinical relevance. Brain 2002;125:1676-95. [ DOI:10.1093/brain/awf177] 19. D'Souza CA, Moscarello MA. Differences in susceptibility of MBP charge isomers to digestion by stromelysin-1 (MMP-3) and release of an immunodominant epitope. Neurochem Res 2006;31:1045-54. [ DOI:10.1007/s11064-006-9116-9] 20. Surgent OJ, Dadalko OI, Pickett KA, Travers BG. Balance and the brain: A review of structural brain correlates of postural balance and balance training in humans. Gait Posture 2019;71:245-52. [ DOI:10.1016/j.gaitpost.2019.05.011] 21. Zhang H, Kim Y, Ro EJ, Ho C, Lee D, Trapp BD, et al. Hippocampal neurogenesis and neural circuit formation in a cuprizone-induced multiple sclerosis mouse model. J Neurosci 2020;40:447-58. [ DOI:10.1523/JNEUROSCI.0866-19.2019] 22. Dunn SE, Gunde E, Lee H. Sex-Based Differences in Multiple Sclerosis (MS): Part II: Rising Incidence of Multiple Sclerosis in Women and the Vulnerability of Men to Progression of this Disease. Curr Top Behav Neurosci 2015;26:57-86. [ DOI:10.1007/7854_2015_370] 23. Norkute A, Hieble A, Braun A, Johann S, Clarner T, Baumgartner W, et al. Cuprizone treatment induces demyelination and astrocytosis in the mouse hippocampus. J Neurosci Res 2009;87:1343-55. [ DOI:10.1002/jnr.21946] 24. Sriram K, Benkovic SA, Hebert MA, Miller DB, O'Callaghan JP. Induction of gp130-related cytokines and activation of JAK2/STAT3 pathway in astrocytes precedes up-regulation of glial fibrillary acidic protein in the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine model of neurodegeneration: key signaling pathway for astrogliosis in vivo? J Biol Chem 2004;279:19936-47. [ DOI:10.1074/jbc.M309304200] 25. Kipp M, Beyer C. Impact of sex steroids on neuroinflammatory processes and experimental multiple sclerosis. Front Neuroendocrinol 2009;30:188-200. [ DOI:10.1016/j.yfrne.2009.04.004] |
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Rashnavadi M, Shakarami N, Soltani S, Azizian H, Nooraei A. Investigating the morphology, morphometry and behavioral analysis of the effect of demyelination on the hippocampus of rats in the model of multiple sclerosis induced by cuprizone. MEDICAL SCIENCES 2024; 34 (4) :385-393 URL: http://tmuj.iautmu.ac.ir/article-1-2212-en.html
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