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Quantitative evaluation of T-Bet gene expression in patients with celiac disease on a gluten-free diet
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Fatemeh Ganjali1   , Mohammad Rostami-Nejad2 , , Hamid Asadzadeh Aghdaei3 , Mehrdad Hashemi 4 |
1- Department of Cellular & molecular biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Associate Professor, Tehran, Iran
3- Gastroenterology and Liver Diseases Research Center, Research Institute for Gastroenterology and Liver Diseases, Shahid Beheshti University of Medical Sciences, Assistant Professor, Tehran, Iran
4- Farhikhtegan Medical Convergence Science Research Center, Farhikhtegan Hospital Tehran Medical sciences, Islamic Azad University, Tehran, Iran , Department Genetics, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Professor, Tehran, Iran , mhashemi@iautmu.ac.ir |
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Abstract: (258 Views) |
Background and Aim: Based on previous studies, T-bet can be effective in inflammation and worsening of celiac disease by improving the progression of Th1-mediated responses and the production of pro inflammatory cytokines such as interferon-gamma (IFNγ) and tumor necrosis factor-alpha (TNF-α). The aim of this study was to evaluate the expression of T-bet gene in patients with celiac disease on a gluten-free diet for 6 months or more compared with healthy individuals.
Methods: In this study, 20 peripheral blood samples were collected from patients (37.5±16.07) with celiac disease and 20 samples from healthy individuals (36.47±8.62) as a control group. After RNA extraction and cDNA synthesis, a specific primer pair of T-bet gene was designed and used after confirmation with blast software. PCR was performed and then T-bet gene expression was assessed by real-time PCR.
Results: In this study, 12 (60%) females and 8 (40%) males in the patient group and 13 (65%) females and 7 (35%) males in the control group were studied. T-bet gene expression was not significantly different in patients with celiac disease on a gluten-free diet for 6 months or more compared with healthy individuals (Pvalue: 0.27).
Conclusion: The results of quantitative analysis of the expression of this gene in patients with celiac disease treated with gluten-free diet (for 6 months or more) compared with controls showed that this gene cannot be used as a diagnostic biomarker to differentiate Patients from healthy individuals.
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| Keywords: Celiac Disease, T-bet transcription factor, Gluten Free Diet, DNA Primer, PCR |
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Full-Text [PDF 1009 kb]
(140 Downloads)
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Semi-pilot: case-control |
Subject:
Molecular Biology
Received: 2024/02/12 | Accepted: 2021/12/31 | Published: 2021/12/31
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