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:: Volume 35, Issue 1 (spring 2025) ::
MEDICAL SCIENCES 2025, 35(1): 35-43 Back to browse issues page
Development and validation of a HPLC-UV method of analysis for sulfasalazine in human plasma
Amir Beheshti Maal1 , Seyed Mohammad Alavi1 , Mohsen Amini2 , Hoda Jahandar3
1- PharmD student Department of Pharmaceutical Chemistry, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University (IAUPS), Tehran, Iran
2- Full Professor, Department of Medicinal Chemistry, Faculty of Pharmacy, Tehran University of Medical Sciences (TUMS), Tehran, Iran , moamini@tums.ac.ir
3- Assistant Professor, Department of pharmacognosy, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University (IAUPS), Tehran, Iran
Abstract:   (191 Views)

Background: In this study, a rapid, simple, and advanced reverse phase high-performance liquid chromatography (RP-HPLC) method was developed for the quantification of sulfasalazine in human plasma.

Materials and methods: Sulfasalazine was extracted from plasma matrices using a simple protein precipitation method by acetonitrile. Chromatographic conditions were optimized (mobile phase compositon, flow rate, injection volume and temperature of the oven). The method was validated in protein precipitated human plasma for linearity, selectivity, accuracy, precision, limit of detection, limit of quantification.

Results: The chromatographic separation was conducted on C18 brisa LC2 column (250 mm × 4.6 mm, 5μ) using isocratic elusion with mobile phase consisting of the mixture of acetonitrile: 10mM Ammonium acetate pH adjusted to 4.6 (30:70 v/v) with a flow rate of 1.0 ml/min at ambient temperature. Detection was carried out by UV detector at 361 nm. Calibration curves made in the human plasma were linear in the range of 3.125-50 μg/ml with the value of r2 > 0.9999. The LOD and LOQ was 0.5 and 2.5 µg/ml, repectively.

Conclusion: The developed and validated HPLC-UV method is suitable for accurately determining sulfasalazine levels in pharmacokinetic studies of new formulations.
Keywords: Sulfasalazine, RP-HPLC, Human plasma, Method development, Validation
Full-Text [PDF 856 kb]   (57 Downloads)    
Semi-pilot: Experimental | Subject: Pharmacology
Received: 2024/03/6 | Accepted: 2024/05/28 | Published: 2025/03/30
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Beheshti Maal A, Alavi S M, Amini M, Jahandar H. Development and validation of a HPLC-UV method of analysis for sulfasalazine in human plasma. MEDICAL SCIENCES 2025; 35 (1) :35-43
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Volume 35, Issue 1 (spring 2025) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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