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A review on high-performance liquid chromatography method design and development
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Seyed Mohammad Alavi1    , Amir Beheshti Maal1 , Hoda Jahandar2 |
1- PhD student in Pharmacy, Faculty of Pharmacy, Faculty of Pharmacy, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran
2- Assistant Professor, Department of Medicinal Plants, Pharmaceutical Sciences Research Center, Faculty of Pharmacy, Tehran Medical Sciences Branch, Islamic Azad University, Tehran, Iran , ho_jahandar@yahoo.com |
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Abstract: (28 Views) |
High performance liquid chromatography is a suitable method for the separation, identification and measurement of components in samples. In the pharmaceutical industry, this method is very important for the analysis of impurities and active pharmaceutical ingredients in the quality control of pharmaceutical products, stability studies and evaluation of new formulations. With the daily emergence of new drugs and pharmaceutical formulations, the need to design and develop analysis methods with high-performance liquid chromatography has also increased. The purpose of this review article is to investigate how to develop an analysis method using high-performance liquid chromatography and related influencing factors. The databases including PubMed, Google Scholar, and Google were utilized with the keywords HPLC, HPLC Method Development, HPLC Method optimization, HPLC Method troubleshooting and HPLC Method validation to find articles related to method design and development. Additionally, the book “Principles of Instrumental Analysis” was used as a guide. Factors such as column type, mobile phase composition, HPLC Separation modes, detector, modes of elution and examination of the physicochemical properties of the drug are important parameters in the design and development of a HPLC method.
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| Keywords: High-performance liquid chromatography, Method design and development, Drug analysis |
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Full-Text [PDF 763 kb]
(20 Downloads)
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Semi-pilot: Review |
Subject:
Pharmacology
Received: 2024/05/27 | Accepted: 2024/08/6 | Published: 2025/09/1
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