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Evaluation of cooperative protective effect of sinomenine combined with gastrodin on carbon tetrachloride-induced hepatic injury in male mouse
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Aref Nooraei1    , Marzieh Darvishi2 , Javad Cheraghi3 , Marzieh Havasi4 , Salman Soltani5 |
1- Assistant Professor of Comparative Anatomy and Embryology, Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran
2- Associate Professor of Anatomical Sciences, Department of Basic Sciences, Faculty of Medicine, Ilam University of Medical Sciences, Ilam, Iran
3- Associate Professor of Physiology, Department of Laboratory Sciences, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran , j.cheraghi@ilam.ac.ir
4- PhD Candidate in Biochemistry, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
5- Assistant Professor of Comparative Histology, Department of Histology, Faculty of Veterinary Sciences, Ilam University, Ilam, Iran |
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Abstract: (224 Views) |
Background: As one of the most important organs of the body, the liver is responsible for detoxifying the body, and any liver failure caused by hepatotoxicity leads to acute liver damage, cirrhosis, and inflammation and destruction of hepatocytes. Therefore, this study was done to investigate the supportive effect of sinomenin with gastrodin among cases of liver damage caused by carbon tetrachloride.
Materials and methods: In this study, 48 male mice were used in the weight range of 23 to 30 grams. Then the mice were randomly divided into 8 groups (n= 6 each), including healthy group, healthy group receiving gastrodin, healthy group receiving sinomenin, the healthy group receiving sinomenin and gastrodin, the damage group receiving gastrodin, the damage group receiving sinomenin, the damage group receiving gastrodin and sinomenin, and the acute liver damage group. After the end of the experiment, the animals were killed by enzyme assay and liver histological study.
Results: The toxicity induced by carbon tetrachloride leads to increase in liver enzymes, as well as destruction of hepatocytes, accumulation of fatty tissue in the liver, and inflammation of the liver. Taking sinomenin and gastrodin improved liver enzymes and liver inflammation.
Conclusion: The use of herbal medicines can be a suitable alternative to synthetic medicines that have many side effects, in which the use of sinomenin and gastrodin can replace anti-inflammatory drugs for the liver and the entire digestive system.
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| Keywords: Carbon tetrachloride, Hepatotoxicity, Gastrodin, Sinomenin |
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Full-Text [PDF 2007 kb]
(87 Downloads)
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Semi-pilot: Experimental |
Subject:
Histology
Received: 2024/08/23 | Accepted: 2025/01/7 | Published: 2025/09/1
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Nooraei A, Darvishi M, Cheraghi J, Havasi M, soltani S. Evaluation of cooperative protective effect of sinomenine combined with gastrodin on carbon tetrachloride-induced hepatic injury in male mouse. MEDICAL SCIENCES 2025; 35 (3) :306-313 URL: http://tmuj.iautmu.ac.ir/article-1-2295-en.html
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