1- Department of Microbiology, TeMS.C, Islamic Azad University, Tehran, Iran 2- Department of Microbiology, TeMS.C, Islamic Azad University, Tehran, Iran , Shokouhi@iautmu.ac.ir
Abstract: (234 Views)
Background: There are many reports of antibiotic resistance in ESBL (Extended Spectrum Beta-Lactamase) producing strains of Acinetobacter baumannii including cephalosporins from all over the word. This research aimed to determine the frequency of colistin-resistant genes (mcr-2, mcr-1) and fosfomycin-resistant genes (fos A3) in ESBL-producing and carbapenem-resistant A. baumannii strains. Materials and methods: Acinetobacter baumannii isolates collected from selected hospitals in Tehran were confirmed by detecting the bla OXA-51-lik gene using PCR. Antibiograms were performed using the disk diffusion method, and the combined disk method was used to identify ESBL-producing isolates. The minimum inhibitory concentration (MIC) for colistin was obtained using the E-test. The presence of colistin (mcr-1, mcr-2) and fosfomycin (fosA3) resistance genes was evaluated using PCR. Results: Of 219 clinical isolates of A. baumannii, 71 isolates with ESBL and carbapenem resistance were detected. The 100% of A. baumannii isolates were resistant to cephalosporins and piperacillin. The antibiotic resistance to ciprofloxacin, amikacin, gentamicin was evaluated at 87.3%, 71.8%, and 67.6%, respectively. The ESBL-producing and carbapenem-resistant isolates were 100% sensitive to colistin, fosfomycin and tigecycline. In these strains, fosA3, mcr-1, and mcr-2 genes were not detected. Conclusion: In the present study, common genes that cause resistance to the antibiotics fosfomycin and colistin were not observed among ESBL-producing and carbapenem-resistant A.baumannii isolates. Therefore, prescribing these antibiotics is still effective and their excessive use should be avoided.
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Nikbakht M, Rahimi M K, Taghizadeh Marand S, Shokouhi Mostafavi S K. Evaluation of prevalence of colistin and fosfomycin resistance genes in the clinical Acinetobacter baumannii isolates producing carbapenemase and ESBL. MEDICAL SCIENCES 2025; 35 (3) :314-322 URL: http://tmuj.iautmu.ac.ir/article-1-2302-en.html