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Comparative study of the effects of biologically synthesized copper and zinc oxide quantum nanodots on biofilm-forming genes in Klebsiella pneumoniae and Staphylococcus aureus bacteria
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Yasman Elsadat Nabipour1    , Ardeshir Hesampour2 , Salman Ahmady Asbchin3 , Maryam Tajabadi1 , Arman Rostamzad4 |
1- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , ardeshir.hesampour@iau.ac.ir
3- Department of Biology, Faculty of Basic Sciences, University of Mazandaran, Babolsar, Iran
4- Department of Biology, Faculty of Basic Sciences, University of Ilam, Ilam, Iran |
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Abstract: (367 Views) |
Background: In the current context, the rise of multidrug-resistant (MDR) pathogenic bacteria has become a major threat to the healthcare sector. Biofilm formation is a key factor in the emergence of MDR bacterial infections. The present study aimed to investigate the effects of copper quantum dots (CuQDs) and green-synthesized zinc oxide quantum dots (ZnOQDs) on the expression of biofilm-related genes in Staphylococcus aureus and Klebsiella pneumoniae.
Materials and methods: This study was designed to compare the effects of ZnOQDs and CuQDs—semiconducting nanoparticles smaller than 10 nm—on drug-resistant Gram-positive and Gram-negative pathogenic bacteria. Acorn fruit extract was used as a reducing and stabilizing agent in the green hydrothermal synthesis of the quantum dots. The minimum inhibitory concentration (MIC) for both types of QDs was determined. Subsequently, the expression levels of biofilm-associated genes in S. aureus and K. pneumoniae were assessed using real-time PCR analysis.
Results: The findings demonstrated that at sub-MIC concentrations—100 µg/mL for ZnOQDs and 200 µg/mL for CuQDs—the expression levels of biofilm-related genes icaA, icaD, and icaR in S. aureus, and wzm in K. pneumoniae, were significantly downregulated compared to the reference gene 16S rRNA.
Conclusion: It is concluded that CuQDs and ZnOQDs could be effective on the expression of biofilm-related genes in Staphylococcus aureus and Klebsiella pneumoniae |
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| Keywords: Quantum dots, Gene expression, Pathogen, Biofilm. |
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Full-Text [PDF 672 kb]
(84 Downloads)
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Semi-pilot: Experimental |
Subject:
Microbiology
Received: 2025/04/29 | Accepted: 2025/10/8 | Published: 2026/07/1
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Nabipour Y E, Hesampour A, Ahmady Asbchin S, Tajabadi M, Rostamzad A. Comparative study of the effects of biologically synthesized copper and zinc oxide quantum nanodots on biofilm-forming genes in Klebsiella pneumoniae and Staphylococcus aureus bacteria. MEDICAL SCIENCES 2026; 36 (2) :189-199 URL: http://tmuj.iautmu.ac.ir/article-1-2381-en.html
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