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Study of p53 and sirtuin1 gene expression levels in sperm of infertile men compared to healthy men
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Fariba Ghodrati1    , Kazem Parivar2 , Iraj Amiri3 , Nasim Hayati Roodbari1 |
1- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran , kparivar.srbiau@gmail.com
3- Department of Anatomy and Embryology, Hamadan University of Medical Sciences, Hamadan, Iran |
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Abstract: (221 Views) |
Background: Several factors, including molecular and genetic changes, play a role in male infertility. The p53 and SIRT1 genes are among the key molecules that play a role in regulating cellular processes such as apoptosis, DNA repair, and oxidative stress. The aim of this study was to investigate the expression levels of p53 and SIRT1 genes in the sperm of infertile men compared to healthy men.
Materials and methods: This case-control study used sperm samples from 15 fertile and 15 infertile men who referred to Omid Infertility Clinic in Hamedan for infertility-related tests. Some of the above sperm were used for fertilization and the other part was frozen for molecular studies. The expression levels of genes were measured using the RT-qPCR technique. After performing IVF and ICSI in vitro fertilization, the results were compared with the results of molecular studies.
Results: The p53 gene expression in the sperm of infertile men was significantly increased (P ≤0.0001), while SIRT1 gene expression was significantly decreased (P ≤0.0001). In terms of the correlation between morphology, sperm count and motility in the semen sample and blastocyst rate obtained from IVF and ICSI, no significant correlation was found with P53 and SIRT1 gene expression.
Conclusion: The findings showed that increased p53 expression causes sperm apoptosis and decreased SIRT1 expression exacerbates oxidative stress; both of which can be used as key markers in the evaluation of male fertility.
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| Keywords: p53, Sirtuin1, Sperm, Infertile men |
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Full-Text [PDF 727 kb]
(85 Downloads)
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Semi-pilot: Experimental |
Subject:
Cilinical Biochemistry
Received: 2025/05/6 | Accepted: 2025/09/3 | Published: 2026/03/30
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