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Study of apoptosis induced by Calendula officinalis extract on experimental colon carcinoma in rats
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Yousef Doustar   1, Dariush Mohajeri , Fatheme Fathiazad , Ali Namvaran |
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Abstract: (23416 Views) |
Background: In vitro studies have demonstrated the anticancer effect of Calendula officinalis extract on tumor cell line derived from colorectal cancers. The inhibition ranged from 70-100%. The aim of this study was to evaluate the apoptosis in epiththelial displastic colon cells following treated with Calendula officinalis extract.
Materials and methods: In this experimental study, 56 wistar male rats with age of 12 weeks and 200-300g weight were allocated into two equal groups of treatment and control. For induction of colorectal carcinoma, these two groups were given 1,2-dimethylhydrazine (40mg/kg), as subcutaneous injection, twice a week for two weeks. Treatment group also was treated with Calendula officinalis extract (200mg/kg/day) orally for 10 weeks. After 10 weeks, distal parts of colon tissue were biopsied in both groups and 3-4 micron tissue section was prepared through TUNEL staining method.
Results: Immunohistopathological study reveals that apoptotic cells of displastic colon epithelial cells in treatment group were higher than control group. Mean difference between treatment and control groups were statistically significant (p<0.01).
Conclusion: This study indicated that Calendula officinalis extract would be induced apoptosis of displastic colon cells in experimental colorectal carcinoma of rats. |
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| Keywords: Apoptosis, Colorectal carcinoma, Calendula officinalis |
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Full-Text [PDF 1334 kb]
(3433 Downloads)
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Subject:
Nutrition Sciences
Received: 2010/08/17 | Published: 2010/04/15
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