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The role and effect of finasteride on the spermatogenesis, prostate gland and epididym of mature NMRI mouse in in vivo and in vitro conditions
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Kazem Parivar   1, Parichehr Yaghmaei , Sahar Sekhavati |
| 1- , kazem_parivar@yahoo.com |
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Abstract: (36000 Views) |
Background: Finasteride, a 4-azasteroid compound, is a specific inhibitor of type P 5α-reductase that converts testosterone into 5α-dihydrotestosterone. In the present study, the effects of human dose of finasteride on the number of spermatogenic cells, seminiferous, prostatic and epididymal tubules diameter and thickness of mature NMRI mouse were investigated, both in in vivo and in vitro conditions.
Material and methods: In this experimental study, in in vivo condition, 18 mature NMRI mice were divided into 3 groups of 6: control (without treatment), sham (treatment whit physiologic serum) and experimental (treatment with drug). Experimental group was received intraperitoneal injection of 5 mg/kg/day finasteride for 7 days. In in vitro condition, testes, prostates and epididyms of 18 mature NMRI mice were divided into 3 groups of 6: control (fixation with bouin without treatment), sham (treatment with physiologic serum) and experimental (treatment with 5 mg/kg/day of finasteride in culture media for 3 days). Data were analyzed using one way ANOVA and Tukey test by SPSS software. P- value< 0.05 was considered significant.
Results: In in vivo condition, treatment with finasteride did not cause significant reduction in the number of spermatogenic cells, seminiferous tubules diameter compared with control group. But, in in vitro condition, significant decrease was observed. In both conditions, the drug could cause significant decrease in prostatic tubules diameter and thickness compared with control group. Diameter and thickness of epididymis tubules were decreased just in in vitro condition.
Conclusion: Finasteride can act in long-term treatment, high doses and in in vitro condition better than other conditions. |
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| Keywords: Finasteride, Dihydrotestosterone, Prostate, Spermatogenesis, Epididymis, NMRI mouse. |
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Full-Text [PDF 334 kb]
(3676 Downloads)
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Semi-pilot: Experimental |
Subject:
Pharmacology
Received: 2010/11/16 | Published: 2010/11/15
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