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Evaluation of the enterococcus role in treatment of infectious diseases produced by Staphylococcus aureus
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Mahsa Yeganeh   1, Anita Khanafari2 , Mohammad reza Fallahian3 , Anoosheh Sharifan4 |
1- MSc of Food Science and Technology, Department of Food Science and Technology, Islamic Azad University, Sciences and Researches Campus, Tehran, Iran , mahsayeganeh19@yahoo.com
2- Associated professor of Microbiology, Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
3- Instructor of Microbiology, Department of Microbiology, Islamic Azad University, Tehran North Branch, Tehran, Iran
4- Assistant professor of Food Science and Technology, Department of Food Science and Technology, Islamic Azad University, Sciences and Researches Campus, Tehran, Iran |
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Abstract: (17148 Views) |
Background: The aim of this study was to evaluate the Enterococcus role in treatment of infectious diseases produced by Staphylococcus aureus by producing of Tyrothricin antibiotic.
Materials and methods: After the culture of Enterococcus faecalis (PTCC 1394) in the BHI agar, centrifuging and isolation of microbial mass, antimicrobial compound of this strain was purified by dialysis and molecular weight was estimated by SDS-PAGE electrophoresis. Antimicrobial effect and minimum inhibitory concentration of antibiotic was evaluated.
Results: Molecular weight of antimicrobial compound was approximately 66 KDa. It was similar to molecular weight of tyrothricin as antimicrobial compound of Enterococcus. After dialysis, activity unit, specific activity, purification factor increased and total activity, amount of total protein decreased. The most antimicrobial effect and the lowest inhibitory concentration produced by tyrothricin were against Staphylococcus aureus.
Conclusion: Enterococcus faecalis (PTCC1394) can produce antimicrobial compound with broad specterum, so it can play important role in treatment of angina and intestinal inflammations. |
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| Keywords: Antimicrobial effect, Angina, Enterococcus faecalis, Intestinal inflammations, Tyrothricin |
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Full-Text [PDF 153 kb]
(2375 Downloads)
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Semi-pilot: Experimental |
Subject:
Microbiology
Received: 2012/02/15 | Published: 2012/02/15
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