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Development of non-enzymatic amperometric biosensor for the determination of cysteine and acetyl cysteine
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Naghmeh Sattarahmady1   , Hossein Heli2 |
1- PhD of Biophysics, Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran, Department of Medical Physics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
2- PhD of Electrochemistry, Institute of Mechanics, Iranian Space Research Center, Shiraz, Iran , hheli7@yahoo.com |
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Abstract: (12806 Views) |
Background: Extensive researches exist in the recent years about attractive properties of nanoparticles and their applicability in design of the biosensors. In this area, development of simple and reliable biosensors for the determination of sulfhydryl compound is pertinent. In this trial, an enzyme-less amperometric biosensor was designed for the detection of cysteine and N-acetyl-L-cysteine.
Materials and methods: Nanoparticles of iron (III) oxide core-cobalt hexacyanoferrate shell were synthesized and employed as a signal transducer in the amperometric biosensor.
Results: The nanoparticles represented a good redox behavior and represented an efficient catalytic activity toward the oxidation of the substrates.
Conclusion: Amperometric signals of the biosensor were successfully recorded for quantification of the substrates and the biosensor was successfully employed for the analysis of real samples or determination in blood serum and urine. |
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| Keywords: Biosensor, Nanoparticle, Cysteine, N-acetyl-L-cysteine |
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Full-Text [PDF 401 kb]
(3394 Downloads)
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Semi-pilot: Experimental |
Subject:
Medical Physics
Received: 2013/03/16 | Accepted: 2013/05/28 | Published: 2013/05/28
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