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Introduction of Liver Infusion Tryptose (LIT) for cultivation and diagnosis of Blastosystis sp.
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Ali Moosavi1   , Ali Haghighi1 , Mohammad Rostami-Nejad2 , Ehsan Nazemalhosseini Mojarad3 , Farideh Naderi1 , Anita Mohammadiha1 , Raheleh Rafiei Sefid Dashti4 |
1- Department of Medical Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
2- Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
3- Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , ehsanmojarad@gmail.com
4- Department of Medical Parasitology and Mycology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran |
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Abstract: (9653 Views) |
Background: According to some researches in recent years, Blastocystis is considered as the etiological agent of some gastrointestinal disorders. This parasite is polymorphic and its detection is not easy under the microscope. Because of this problem, several microscopic based studies have already reported different Blastocystis infection rates. Application of culture methods increases the chance of diagnosis of this parasite. The in vitro culture of Blastocystis in Liver Infusion Tryptose (LIT) medium is described for the first time in this study.
Materials and Methods: Liver Infusion Tryptose (LIT) containing 10% fetal calf serum in screw-capped tubes was used. Samples incubated in 37°c and examined after 24, 48 and 72 hours.
Results: 420 fresh stool samples were tested by LIT culture method and 100 positive samples (23.8%) of Blastocystis sp. were detected. while only 64 samples (15.2%) were positive with direct smear examination.
Conclusion: This study highlights the same sensitivity of this culture media and other regular cultures media and it introduces a new medium for Blastocystis sp. detection. |
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| Keywords: Blastocystis, Culture media, Diagnosis |
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Full-Text [PDF 130 kb]
(5811 Downloads)
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Semi-pilot: Experimental |
Subject:
Parasitology
Received: 2014/01/22 | Accepted: 2014/01/22 | Published: 2014/01/22
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