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:: Volume 28, Issue 2 (winter 2018) ::
MEDICAL SCIENCES 2018, 28(2): 92-103 Back to browse issues page
Differentiation potential of human CD133 and CD34 positive hematopoietic stem cells into motor neuron- like cells; an in vitro study
Sepideh Alavi Moghaddam1, Faezeh Faghihi *2, Nasim Hayati Roodbari3
1- Cellular and Molecular Biology, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
2- Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran. , faezefaghihi@yahoo.com
3- Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Abstract:   (1005 Views)
Background: We were studied on differentiation potential of CD133+ and CD34+ hematopoietic stem cells into motor neuron like cells.
Materials and methods: CD133+ and CD34+ HSCs were isolated from human UCB using MACS system. After cell characterization using flow cytometry, the cells were treated with a combination of Retinoic acid, Sonic hedgehog, Brain derived neurotrophic factor, and B27 through a 2- step procedure for two weeks. The expression of MN-specific markers was examined using immunocytochemistry and flow cytometery.
Results: Flow cytometery analysis revealed 98% and 95.7% CD133+ and CD34+ cells, respectively. By the end of the two-week differentiation protocol, CD133+ and CD34+ cells acquired unipolar MNL morphology with thin and long neurites. The expression of Isl-1, AChE, NF-H and Nestin was detected in 66.4%, 58.3%, 80.6%and 84.9% of CD133+ cells and 63.2%, 52.3%, 78.6% and 80.1% of CD34+ cells. T
Conclusion: Human UCB - CD133+ and CD34+HSCs are remarkably potent cell candidates to trans- differentiate into motor neuron-like cells, in vitro.
Keywords: CD133+, CD34+, Hematopoietic Stem Cell, Motor Neuron, Umbilical Cord Blood
Full-Text [PDF 1077 kb]   (243 Downloads)    
Semi-pilot: Experimental | Subject: Biology
Received: 2017/10/3 | Accepted: 2017/12/26 | Published: 2018/06/19
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Alavi Moghaddam S, Faghihi F, Hayati Roodbari N. Differentiation potential of human CD133 and CD34 positive hematopoietic stem cells into motor neuron- like cells; an in vitro study. MEDICAL SCIENCES. 2018; 28 (2) :92-103
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Volume 28, Issue 2 (winter 2018) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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