:: Volume 26, Issue 1 (spring 2016) ::
MEDICAL SCIENCES 2016, 26(1): 16-21 Back to browse issues page
Antimutagenicity and cytotoxic effects of Malus domestica peel extract
Maliheh Entezari
of Developmental Biology, Department of Cellular and Molecular Biology, Faculty of Advanced Medical Sciences, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran , mentezari@iautmu.ac.ir
Abstract:   (4754 Views)

Background: At present, cancer is one of the leading causes of mortality in the world. Therefore, prevention can be considered as important as treatment in the management of cancer. Diet can play a vital role in cancer prevention. Nowadays, scientists are looking for natural food which can prevent the cancer occurrence. The purpose of this research was to examine antimutagenicity and anticancer effects of apple peel extract.

Materials and methods: In this experimental study, HUT-78 cell line were cultured in 90% RPMI1640, supplemented with 10% fetal calf serum, l- glutamine, peniciline, streptomycin and then incubated at 37°c for 2 days. The cancer cell line was treated by different apple peel extract concentrations and cellular vital capacity was determined by MTT assay. The apple peel extract was subsequently evaluated in terms of antimutagenicity and anticancer properties by a standard reverse mutation assay (Ames test).

Results: During MTT assay, T cell lymphoma cancerous cells revealed significant cell death compared with controls (p<0.01). In Ames test, the apple peel extract prevented the reverted mutations and the hindrance percent was 81.3%.

Conclusion: The results demonstrated that apple peel extract has anticancer effects in in vitro condition. So, we can persuade people to add apple peel to daily diet.

Keywords: Antimutagenicity, Anticancer, Apple peel, Human Tcell lymphoma cancerous cells, Ames Test.

Keywords: Antimutagenicity, Anticancer, Apple peel, Human Tcell lymphoma cancerous cells, Ames Test.
Full-Text [PDF 244 kb]   (1859 Downloads)    
Semi-pilot: Experimental | Subject: Molecular Biology
Received: 2015/08/23 | Accepted: 2015/11/8 | Published: 2016/03/14


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