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The expression studies of Bcl-xl gene subsequent effect of cholestasis and treatment by curcumin in hippocampus of male rats
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Somayeh Baghbaderani1   , Mohammad Nasehi 2, Mehrdad Hashemi3 |
1- Master of Science, Department of Genetics, Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran
2- Department of Physiology, Institute for Cognitive Science Studies (ICSS), Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran , nasehi@iricss.org
3- Department of Genetics, Tehran Medical Science Branch, Islamic Azad University, Tehran, Iran |
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Abstract: (5040 Views) |
Background: Cholestasis is a hepatic disease, which discomposes the secretion of bile and gives rise for the aggregation of bile compounds such as bile salts, in case of non-treatment, cholestasis finds the capability to influence on different organs such as heart and brain. Besides, the disease is also quite effective on the expression rate of apoptosis and mitochondrial biogenesis genes. Thus, we went through researching about the effects of cholestasis over the changes of expressions for genes run through apoptosis (BCL-XL) in hippocampus region of male rats. We also tried for fact-finding about the impact of curcumin drug, which holds treatment impact for chronic disease such as neoplastic, inflammatory, and nervous disorders over these genes expression in rats’ hippocampus.
Materials and methods: The animals were divided in 4 groups of BDL, BDL- Curcumin, Sham- Curcumin, and Control. Rats of BDL group experienced BDL surgery (closing bile tubes). Besides, of surgery, rats of BDL-Curcumin group, treated with curcumin. Animals of Sham-Curcumin just received surgery stress and treated with drug, while the animals of CONTROL just experienced surgery stress without any other interference. Then, we removed hippocampus from rats’ brains and after the RNA extraction and cDNA synthesis, we investigated genes expression measurement through Real Time PCR technique.
Results: Curcumin drug increased the rate of BCL-XL gene expression at the rats’ hippocampus.
Conclusion: The data indicated that curcumin can alter apoptosis-induced by cholestasis at the hippocampus region. |
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| Keywords: Cholestasis, BCL-XL, Apotosis, Hippocampal, Curcumin. |
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Full-Text [PDF 387 kb]
(3586 Downloads)
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Semi-pilot: Experimental |
Subject:
Genetic
Received: 2017/05/14 | Accepted: 2017/09/6 | Published: 2018/04/1
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Baghbaderani S, Nasehi M, Hashemi M. The expression studies of Bcl-xl gene subsequent effect of cholestasis and treatment by curcumin in hippocampus of male rats
. MEDICAL SCIENCES 2018; 28 (1) :37-43 URL: http://tmuj.iautmu.ac.ir/article-1-1371-en.html
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