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Effects of Phoenix dactylifera extract and testosterone enanthate with and without resistance training on MFN2, FISS and DRP1 genes expression in liver of rat
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Zahra Alikaei1   , Mohammad Ali Azarbayjani  2, Sirvan Atashak3 , Maghsoud Peeri1 , Saleh Rahmati-Ahmadabad4 |
1- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2- Department of Exercise Physiology, Central Tehran Branch, Islamic Azad University, Tehran, Iran , m_azarbayjani@iauctb.ac.ir
3- Department of Physical Education, Mahabad Branch, Islamic Azad University, Mahabad, Iran
4- Department of Physical Education, Pardis Branch, Islamic Azad University, Pardis, Iran |
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Abstract: (870 Views) |
Background: The use of testosterone enanthate has increased among athletes, especially in resistance disciplines. Considering the side effects of using testosterone enanthate, it seems necessary to find alternative methods. The present study compared the independent and interactive effects of resistance training, testosterone and palm pollen extract on mRNA expression of MFN2, FISS and DRP1 genes in male rat liver.
Materials and methods: In a experimental study, 30 male rats were divided into control groups, resistance training, date pollen extract, testosterone ethanate, consumption of date pollen extract + resistance training and testosterone etantate + resistance training. The interventions were applied based on the group name for four weeks. 48 hours after the last intervention, the liver tissue was investigated to measure the expression of MFN2, FISS and DRP1 genes.
Results: Exercise, testosterone and date pollen, each independently, increased the expression of MFN2 gene and decreased the expression of FISS and DRP1 genes in the liver. The simultaneous use of exercise and testosterone/palm pollen strengthened the effect of independent interventions; however, the synergistic effect was observed only on liver FISS gene expression compared to independent interventions.
Conclusion: According to the results of the groups consuming date pollen and testosterone, it seems that the use of each of them has caused a similar pattern of change in the variables of the present study; therefore, the use of date pollen may be a natural alternative to testosterone.
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| Keywords: Resistance training, Liver, Testosterone, Palm pollen extract, Gene expression |
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Full-Text [PDF 365 kb]
(447 Downloads)
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Semi-pilot: Experimental |
Subject:
Physiology
Received: 2022/07/8 | Accepted: 2022/10/11 | Published: 2023/03/30
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1. Cartoni R, Léger B, Hock MB, Praz M, Crettenand A, Pich S, et al. Mitofusins 1/2 and ERRalpha expression are increased in human skeletal muscle after physical exercise. J Physiol 2005;567:349-58.
2.Delroz H, Abdi A, Barari A, Farzanegi P. The Effect of Eight Weeks of Aerobic Training Combined With Resveratrol on MFn1 and MFn2 Expression in Cardiac Myocytes in a Non-alcoholic Fatty Liver Animal Model. CMJA 2020;9:3878-89.
https://doi.org/10.32598/cmja.9.3.627.3
3.Joseph AM, Pilegaard H, Litvintsev A, Leick L, Hood DA. Control of gene expression and mitochondrial biogenesis in the muscular adaptation to endurance exercise. Essays Biochem 2006; 42:13-29.
https://doi.org/10.1042/bse0420013
4.Moore TM, Zhou Z, Cohn W, Norheim F, Lin AJ, Kalajian N, et al. The impact of exercise on mitochondrial dynamics and the role of Drp1 in exercise performance and training adaptations in skeletal muscle. Mol Metab 2019;21:51-67.
https://doi.org/10.1016/j.molmet.2018.11.012
5.Zandi A, Azarbayjani MA, Peeri M, Hosseini SA. The Effect of Aerobic Training and Ozone Therapy on the Levels of MFN1 and DRP1 Gene Expression in the Heart Tissue of Rats with Osteoarthritis. Gene Cell Tissue 2020;7:e106920.
https://doi.org/10.5812/gct.106920
6.Fealy CE, Mulya A, Lai N, Kirwan JP. Exercise training decreases activation of the mitochondrial fission protein dynamin-related protein-1 in insulin-resistant human skeletal muscle. J Appl Physiol (1985) 2014;117:239-45.
https://doi.org/10.1152/japplphysiol.01064.2013
7.Xue R, Zhu X, Jia L, Wu J, Yang J, Zhu Y, et al. Mitofusin2, a rising star in acute-on-chronic liver failure, triggers macroautophagy via the mTOR signalling pathway. J Cell Mol Med 2019;23:7810-18.
https://doi.org/10.1111/jcmm.14658
8.Xue R, Yang J, Jia L, Zhu X, Wu J, Zhu Y, et al. Mitofusin2, as a Protective Target in the Liver, Controls the Balance of Apoptosis and Autophagy in Acute-on-Chronic Liver Failure. Front Pharmacol 2019;10:601.
https://doi.org/10.3389/fphar.2019.00601
9.Hernández-Alvarez MI, Sebastián D, Vives S, Ivanova S, Bartoccioni P, Kakimoto P, et al. Deficient Endoplasmic Reticulum-Mitochondrial Phosphatidylserine Transfer Causes Liver Disease. Cell 2019;177:881-95.e17.
https://doi.org/10.1016/j.cell.2019.04.010
10.Zhou H, Zhu P, Wang J, Toan S, Ren J. DNA-PKcs promotes alcohol-related liver disease by activating Drp1-related mitochondrial fission and repressing FUNDC1-required mitophagy. Signal Transduct Target Ther 2019;4:56.
https://doi.org/10.1038/s41392-019-0094-1
11.Wei Q, Ramsey SA, Larson MK, Berlow NE, Ochola D, Shiprack C, et al. Elucidating the transcriptional program of feline injection-site sarcoma using a cross-species mRNA-sequencing approach. BMC Cancer 2019;19:311.
https://doi.org/10.1186/s12885-019-5501-z
12.Porcellato I, Menchetti L, Brachelente C, Sforna M, Reginato A, Lepri E, et al. Feline Injection-Site Sarcoma. Vet Pathol 2017;54:204-11.
https://doi.org/10.1177/0300985816677148
13.Pronsato L, Boland R, Milanesi L. Testosterone exerts antiapoptotic effects against H2O2 in C2C12 skeletal muscle cells through the apoptotic intrinsic pathway. J Endocrinol 2012;212:371-81.
https://doi.org/10.1530/JOE-11-0234
14.Pronsato L, Milanesi L. Effect of testosterone on the regulation of p53 and p66Shc during oxidative stress damage in C2C12 cells. Steroids 2016;106:41-54.
https://doi.org/10.1016/j.steroids.2015.12.007
15.Kelly DM, Jones TH. Testosterone: a metabolic hormone in health and disease. J Endocrinol 2013;217:R25-45.
https://doi.org/10.1530/JOE-12-0455
16.An Q, Gu YQ. Testosterone replacement therapy: Dilemmas and challenges in China and Asia. Asian J Androl 2018;20:149-51.
https://doi.org/10.4103/aja.aja_16_17
17.Petering RC, Brooks NA. Testosterone Therapy: Review of Clinical Applications. Am Fam Physician 2017;96:441-9.
18.Osterberg EC, Bernie AM, Ramasamy R. Risks of testosterone replacement therapy in men. Indian J Urol 2014;30:2-7.
https://doi.org/10.4103/0970-1591.124197
19.Howatson G, McHugh MP, Hill JA, Brouner J, Jewell AP, van Someren KA, et al. Influence of tart cherry juice on indices of recovery following marathon running. Scand J Med Sci Sports 2010;20:843-52.
https://doi.org/10.1111/j.1600-0838.2009.01005.x
20.Bell PG, Walshe IH, Davison GW, Stevenson EJ, Howatson G. Recovery facilitation with Montmorency cherries following high-intensity, metabolically challenging exercise. Appl Physiol Nutr Metab 2015;40:414-23
https://doi.org/10.1139/apnm-2014-0244
21.Levers K, Dalton R, Galvan E, O'Connor A, Goodenough C, Simbo S, et al. Effects of powdered Montmorency tart cherry supplementation on acute endurance exercise performance in aerobically trained individuals. J Int Soc Sports Nutr 2016;13:22.
https://doi.org/10.1186/s12970-016-0133-z
22.Stefanescu R, Tero-Vescan A, Negroiu A, Aurica E, Vari CE. A Comprehensive Review of the Phytochemical, Pharmacological, and Toxicological Properties of Tribulus terrestris L. Biomolecules 2020;10:752.
https://doi.org/10.3390/biom10050752
23.Ghanbari-Niaki A, Rahmati-Ahmadabad S. Effects of a fixed-intensity of endurance training and pistacia atlantica supplementation on ATP-binding cassette G4 expression. Chin Med. 2013;8:23.
https://doi.org/10.1186/1749-8546-8-23
24.Rahmati-Ahmadabad S, Azarbayjani M, Nasehi M. The Effects of High-Intensity Interval Training with Supplementation of Flaxseed Oil on BDNF mRNA Expression and Pain Feeling in Male Rats. Ann Appl Sport Sci 2017;5:1-12.
https://doi.org/10.29252/aassjournal.5.4.1
25.Rahmati-Ahmadabad S, Azarbayjani MA, Farzanegi P, Moradi L. High-intensity interval training has a greater effect on reverse cholesterol transport elements compared with moderate-intensity continuous training in obese male rats. Eur J Prev Cardiol 2019:2047487319887828.
https://doi.org/10.1177/2047487319887828
26.Tahvilzadeh M, Hajimahmoodi M, Rahimi R. The Role of Date Palm (Phoenix dactylifera L) Pollen in Fertility: A Comprehensive Review of Current Evidence. J Evid Based Complementary Altern Med 2016;21:320-4.
https://doi.org/10.1177/2156587215609851
27.Selmani C, Chabane D, Bouguedoura N. Ethnobotanical survey of phoenix dactylifera l. Pollen used for the treatment of infertility problems in algerian oases. Afr J Tradit Complement Altern Med 2017;14:175-86.
https://doi.org/10.21010/ajtcam.v14i3.19
28.Mehraban F, Jafari M, Akbartabar Toori M, Sadeghi H, Joodi B, Mostafazade M, et al. Effects of date palm pollen (Phoenix dactylifera L.) and Astragalus ovinus on sperm parameters and sex hormones in adult male rats. Iran J Reprod Med 2014;12:705-12.
29.Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J 2012;5:9-19.
https://doi.org/10.1097/WOX.0b013e3182439613
30.Shirvani H, Rahmati-Ahmadabad S, Broom DR, Mirnejad R. Eccentric resistance training and β-hydroxy-β-methylbutyrate free acid affects muscle PGC-1α expression and serum irisin, nesfatin-1 and resistin in rats. J Exp Biol 2019;222:jeb198424.
https://doi.org/10.1242/jeb.198424
31.Al Jaouni SK, Hussein A, Alghamdi N, Qari M, El Hossary D, Almuhayawi MS, et al. Effects of Phoenix dactylifera Ajwa on Infection, Hospitalization, and Survival Among Pediatric Cancer Patients in a University Hospital: A Nonrandomized Controlled Trial. ntegr Cancer Ther 2019;18:1534735419828834.
https://doi.org/10.1177/1534735419828834
32.Bentrad N, Hamida-Ferhat A. Date palm fruit (Phoenix dactylifera): Nutritional values and potential benefits on health. In: Preedy VR, Watson RR, eds. The Mediterranean Diet. 2nd ed. New York: Academic Press; 2020. p. 239-55.
https://doi.org/10.1016/B978-0-12-818649-7.00022-9
33.Assirey A, Wagih H, Mahran H. Phoenix dactylifera L. Extract Diminished Apoptotic Effect in Cirrhotic Liver of a Rat Model. Int J Pharmacol 2019;15:92-101.
https://doi.org/10.3923/ijp.2019.92.101
34.Roshankhah S, Abdolmaleki A, Salahshoor MR. Anti-inflammatory, anti-apoptotic, and antioxidant actions of Middle Eastern Phoenix dactylifera extract on mercury-induced hepatotoxicity in vivo. Mol Biol Reports 2020;47:6053-65.
https://doi.org/10.1007/s11033-020-05680-4
35.Mirza MB, Elkady AI, Al-Attar AM, Syed FQ, Mohammed FA, Hakeem KR. Induction of apoptosis and cell cycle arrest by ethyl acetate fraction of Phoenix dactylifera L. (Ajwa dates) in prostate cancer cells. J Ethnopharmacol 2018;218:35-44.
https://doi.org/10.1016/j.jep.2018.02.030 [ DOI:10.1113/jphysiol.2005.092031] |
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