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:: Volume 26, Issue 2 (summer 2016) ::
MEDICAL SCIENCES 2016, 26(2): 69-75 Back to browse issues page
Survival and proliferation of pancreatic islets isolated from adult mouse in laboratory culture conditions
Mohammad Nabiouni1 , Elham Havizi2 , Azar Chavoshian3 , Somayeh Bahramzadeh4 , Mahmood Hashemitabar 5
1- Department of Cellular and Molecular, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
2- Department of Biology, Faculty of Sciences, Shahid Chamran University, Ahvaz, Iran
3- MSC in Cellular and Molecular Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran
4- Department of Cellular and Molecular Biology, Developmental Cellular Biology, Jondi-Shapoor University of Medical Sciences, Ahvaz, Iran
5- Cellular and Molecular Research Center, Jondi-Shapoor University of Medical Sciences, Ahvaz, Iran , Hashemi _ tabar @ Hotmail.com
Abstract:   (5640 Views)

Background: Recent advances in directed differentiation of pancreatic stem cells offers potential to the development of replacement therapy for diabetic patients. However, the existing differentiation protocols are complex, time-consuming, and costly; thus there is a need for alternative protocols. Today, co-culturing with pancreatic islets is apparently a promising protocol for producing beta cells. Furthermore, we need keep islets viable in vitro for extended time periods.

Materials and methods: We maintained isolated pancreatic islets obtained from the mouse pancreas in tissue culture for 2 weeks, after which we studied the viability, proliferation and morphology of the islets. Pancreatic islets were isolated from overnight-fasted male NMRI mice by Lacy and Kostianovsky modified collagenase digestion method and islets were tested for their specificity by dithizone (DTZ) staining. Also, islet cell viability was tested by MTT assay.

Results: Our results showed that viable pancreatic islets can be isolated from the pancreas of adult mice and maintained in tissue culture for at least 1 week, without loss of the specific functions of the cells. Cell viability of pancreatic islets was decreased after one week and also, the cell number decreased over time.  Cell viability and cell number were decrease if cells were incubated for long time.

Conclusion: It remains to be established whether such islets will survive and remain functionally competent after co culturing. Survey of viability of pancreatic islets is necessary in in vitro, because these were used for cell therapy for diabetes.

Keywords: Survival, Proliferation, Pancreatic islets, Culture, MTT, NMRI.

Keywords: Survival, Proliferation, Pancreatic islets, Culture, MTT, NMRI.
Full-Text [PDF 508 kb]   (3658 Downloads)    
Semi-pilot: Experimental | Subject: Animal Biology
Received: 2015/08/8 | Accepted: 2015/12/27 | Published: 2016/06/28
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Nabiouni M, Havizi E, Chavoshian A, Bahramzadeh S, Hashemitabar M. Survival and proliferation of pancreatic islets isolated from adult mouse in laboratory culture conditions . MEDICAL SCIENCES 2016; 26 (2) :69-75
URL: http://tmuj.iautmu.ac.ir/article-1-1090-en.html


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Volume 26, Issue 2 (summer 2016) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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