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Time dependent effects of ischemic-reperfusion on rat’s cardiomyocytes apoptosis
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Yoosof Doustar   , Alirteza Gorjani , Mehrdad Hashemi1 , Rambod Rezapour Shahir |
| 1- , hashemi_mehrdad@yahoo.com |
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Abstract: (21065 Views) |
Background: The study objective was to investigate time dependent effects of ischemic- reperfusion on cardiomyocyte apoptosis.
Materials and Methods: Male Sprague-Dawley rats (270-330 gr) were randomly divided into four groups (n=10) and anesthetized by sodium pentobarbital (50-60 mg/kg-IP). The hearts of rats in each of three treatment groups were removed and quickly mounted on a Langendorff apparatus and perfused by a modified Krebs-Henseleit solution under constant pressure at 37ºC. During the stabilization time, 30 minute regional ischemia (which was followed with 60, 90 or 120 minute reperfusion in respective T/60min, T/90 min and T/120 min reperfusion groups) was established, while in the control group the hearts were intact. Immunohistochemical detection of apoptotic cells was performed using an in-situ cell death detection (TUNEL) kit. The positive cardiomyocytes counted in five random high power fields in each sample. Data were presented as mean±SEM for each group.
Results: In the control group, the number of apoptotic cells was 1.0±0.4, while in the treatment groups of T/60min, T/90 min and T/120 min reperfusion, the corresponding numbers were 2.0±0.5, 3.0±0.3 and 6.0±0.3, respectively. Although the difference between T/60 min & T/90 min and T/60 min & control group was not significant (p>0.05), the difference between T/60 min & T/120 min (p<0.001), T/90 min & T/120 min (p<0.01), T/90 min & control (p<0.01) and T/120 min & control (p<0.001) was significant.
Conclusion: We demonstrated that the duration of ischemic-reperfusion insult can affect the apoptosis changes in cardiomyocytes. |
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| Keywords: Ischemia-reperfusion, Apoptosis, Infarction. |
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Full-Text [PDF 222 kb]
(3840 Downloads)
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Subject:
Epidemiology
Received: 2006/09/6 | Published: 2006/09/15
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