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Evaluation of the prevalence of broad-spectrum beta-lactamases (ESBLs) and carbapenemase genes in Klebsiella pneumoniae strains isolated from burn wounds in patients referred to Shahid Motahari Hospital in Tehran
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Ali Shivaee1    , Shahla Shahbazi 2, Armaghan Soltani3 , Elaheh Ahadi4 |
1- MSc in Medical Microbiology, Department of Microbiology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- PhD Student, Department of Bacteriology, Pasteur Institute of Iran, Teheran, Iran , shahbazi.shahla@yahoo.com
3- MSc Student in Microbiology, Department of Bacteriology, Pasteur Institute of Iran, Teheran, Iran
4- MSc Student in Microbiology, Department of Biology, Alborz Branch, Islamic Azad University, Karaj, Iran |
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Abstract: (3044 Views) |
Background: Klebsiella species are common causes of nosocomial, ulcers, blood and urinary tract infections, and also acquired pneumonia from the hospital and various intra-abdominal infections. Bacterial resistance mechanisms against antibiotics are different, but one of these resistance mechanisms, which is very problematic, is the production of β-lactamase enzymes in bacteria. The aim of this study was to determine the antibiotic resistance pattern and the presence of beta-lactamase genes in Klebsiella isolated from burn wounds among patients referred to Shahid Motahhari Hospital in Tehran.
Materials and methods: 100 strains collected for confirmation of production of broad-spectrum beta-lactamases (ESBLs) were tested by CDT (Combined Disk Test). Finally, β-lactamase genes were investigated using polymerase chain reaction.
Results: The highest resistance rate was observed to ampicillin (93%). 23% of isolates produced ESBLs. The highest frequency of genes was bla shv gene (26.8%).
Conclusion: The presence of beta-lactamase genes with high antibiotic resistance is very worrying. Since the present genes can spread through mobile genetic elements in bacteria, among bacteria, it is considered to be a serious alert in the treatment of infections caused by Klebsiella.
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| Keywords: Klebsiella pneumoniae, Antibiotic resistance, Beta-lactamase genes, ESBLs |
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Full-Text [PDF 852 kb]
(1543 Downloads)
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Semi-pilot: Quazi-Experimental |
Subject:
Microbiology
Received: 2018/11/20 | Accepted: 2018/12/5 | Published: 2019/09/28
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