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:: Volume 31, Issue 3 (Fall 2021) ::
MEDICAL SCIENCES 2021, 31(3): 266-275 Back to browse issues page
Evaluation of behavior of human umbilical cord-Wharton’s jelly mesenchymal stem cell on electrospun poly (lactic acid)wax nanofibers scaffold
Tina Shafaf1 , Elham Hoveizi 2, Sayed Reza Kazeminejad3
1- PhD Candidate, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2- Associate Professor, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran , e.hoveizi@yahoo.com
3- Associate Professor, Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
Abstract:   (2175 Views)
Background: Mesenchymal stem cells are pluripotent stromal cells which are capable of differentiating into different cell lines. Nowadays, umbilical cord Wharton’s jelly (UC-WJ) are increasingly used as sources of stem cells. Studies show that scaffolds can affect the differentiation of stem cells to different cells and cause higher cell viability and proliferation as well. The present study aimed to evaluate the adhesion and viability of WJ-MSCs to PLA/Wax scaffold.
Materials and methods: PLA/Wax scaffold was prepared using electrospinning method. Adhesion and viability of MSCs on this scaffold was investigated using scanning electron microscope (SEM) and MTT assay respectively.
Results: SEM results showed that the fibers were homogeneous, uniform, and free of beads with high quality property, and adding wax to PLA significantly reduced the diameter of the nanofibers. These studies confirmed that the cells were attached to the scaffold in large numbers and with appropriate size. The results of MTT show good biocompatibility of the scaffold made with the cells and a significant increase in the survival rate of mesenchymal cells was observed during the period.
Conclusion: In conclusion, using PLA/Wax scaffold has promoted the attachment, survival and proliferation of the cells and has the potential to be an important candidate for developing the efficiency of 3D-cultures in order to cure diseases.
Keywords: Cell viability, PLA/Wax scaffold, Umbilical cord Wharton’s jelly, Adhesion, Mesenchymal stem cells.
Full-Text [PDF 443 kb]   (1649 Downloads)    
Semi-pilot: Experimental | Subject: Animal Biology
Received: 2020/10/31 | Accepted: 2021/04/28 | Published: 2021/09/1
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Shafaf T, Hoveizi E, Kazeminejad S R. Evaluation of behavior of human umbilical cord-Wharton’s jelly mesenchymal stem cell on electrospun poly (lactic acid)wax nanofibers scaffold. MEDICAL SCIENCES 2021; 31 (3) :266-275
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Volume 31, Issue 3 (Fall 2021) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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