[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 31, Issue 4 (WINTER 2021) ::
MEDICAL SCIENCES 2021, 31(4): 388-396 Back to browse issues page
Role of Propolis as a pharmaceutical candidate on interleukin-1β proinflammatory cytokine expression in Alzheimer's rat
Maryam Saeidi1, Fatemeh Rouhollah 2
1- Department of Cellular and Molecular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Cellular and Molecular Sciences, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , Frouhollah@iautmu.ac.ir
Abstract:   (1035 Views)
Background: One of the important factors that play a key role in the pathogenesis of Alzheimer's disease (AD) is inflammatory processes leading to impaired expression of inflammatory cytokines such as interleukin-6, TNF-α, and interleukin-1β (IL-1β). Propolis, as a therapeutic compound, has anti-inflammatory and antioxidant properties. Therefore, the aim of the present study was to investigate the effect of this compound on changes in IL-1β expression levels in AD rats.
Materials and methods: 42 adult male Wistar rats were divided into four groups, including control, sham, Alzheimer, and propolis. Cellular changes in brain tissue was evaluated by preparing histopathologic slides and effect of propolis on changes in IL-1β expression levels were investigated using Real-Time PCR method.
Results: Histopathologic investigations in the studied groups showed a significant difference between scopolamine-treated group compared to control and sham groups in terms of changes in the IL-1β expression levels and cellular damage; whereas there was no difference between the propolis-treated and scopolamine-treated groups in this regard; only-morphological difference in the organization of neurons were observed. The results of Real-Time PCR showed that the IL-1β expression level was significantly different in the propolis-treated group from the Alzheimer's group (p<0.05), indicating that propolis significantly decreased gene expression.
Conclusion: The results of the present study showed that propolis could be considered as an effective drug candidate for the AD treatment considering its anti-inflammatory property and effect on the decrease of IL-1β expression.
Keywords: Alzheimer's disease, Interleukin-1 beta, Propolis, Wistar rats
Full-Text [PDF 802 kb]   (279 Downloads)    
Semi-pilot: Basic | Subject: Brain and Nerves System
Received: 2020/12/27 | Accepted: 2021/08/28 | Published: 2021/12/1
1. Rubio-Perez JM, Morillas-Ruiz JM. A review: inflammatory process in Alzheimer's disease, role of cytokines. ScientificWorldJournal 2012;2012:756357. [DOI:10.1100/2012/756357]
2. Prince M, Albanese E, Guerchet M, Prina M. World Alzheimer Report 2014: dementia and risk reduction: an analysis of protective and modifiable factors. Alzheimer's Disease International 2014.
3. Alzheimer's Association. 2015 Alzheimer's disease facts and figures. Alzheimer's & dementia: J Alzheimers Dis 2015;11:332-84. [DOI:10.1016/j.jalz.2015.02.003]
4. Querfurth HW, LaFerla FM. Mechanisms of disease. N Engl J Med 2010; 362:329-44. [DOI:10.1056/NEJMra0909142]
5. Schmid AW, Lynch MA, Herron CE. The effects of IL‐1 receptor antagonist on beta amyloid mediated depression of LTP in the rat CA1 in vivo. Hippocampus 2009; 19:670-76. [DOI:10.1002/hipo.20542]
6. Su F, Bai F, Zhang Z. Inflammatory cytokines and Alzheimer's disease: a review from the perspective of genetic polymorphisms. Neurosci Bull 2016; 32:469-80. [DOI:10.1007/s12264-016-0055-4]
7. Akiyama H, Barger S, Barnum S, Bradt B, Bauer J, Cole GM, et al. Inflammation and Alzheimer's disease. Neurobiol Aging 2000; 21:383-421. [DOI:10.1016/S0197-4580(00)00124-X]
8. Rao PP, Kabir SN, Mohamed T. Nonsteroidal anti-inflammatory drugs (NSAIDs): progress in small molecule drug development. Pharmaceuticals (Basel) 2010;3:1530-49. [DOI:10.3390/ph3051530]
9. Anand R, Gill KD, Mahdi AA. Therapeutics of Alzheimer's disease: Past, present and future. Neuropharmacology 2014;76:27-50. [DOI:10.1016/j.neuropharm.2013.07.004]
10. Alzheimer's Association. 2014 Alzheimer's disease facts and figures. Alzheimer's & dementia: J Alzheimers Dis 2014;10:e47-92. [DOI:10.1016/j.jalz.2014.02.001]
11. Auld DS, Kornecook TJ, Bastianetto S, Quirion R. Alzheimer's disease and the basal forebrain cholinergic system: relations to β-amyloid peptides, cognition, and treatment strategies. Prog Neurobiol 2002;68:209-45. [DOI:10.1016/S0301-0082(02)00079-5]
12. Farlow MR, Miller ML, Pejovic V. Treatment options in Alzheimer's disease: maximizing benefit, managing expectations. Dement Geriatr Cogn Disord 2008; 25:408-22. [DOI:10.1159/000122962]
13. Simunkova M, Alwasel SH, Alhazza IM, Jomova K, Kollar V, Rusko M,et al. Management of oxidative stress and other pathologies in Alzheimer's disease. Arch Toxicol 2019; 22:1-23. [DOI:10.1007/s00204-019-02538-y]
14. Ha GT, Wong RK, Zhang Y. Huperzine a as potential treatment of Alzheimer's disease: an assessment on chemistry, pharmacology, and clinical studies. Chem Biodivers 2011;8:1189-204. [DOI:10.1002/cbdv.201000269]
15. Polinsky RJ. Clinical pharmacology of rivastigmine: a new-generation acetylcholinesterase inhibitor for the treatment of Alzheimer's disease. Clin Ther 1998;20:634-47. [DOI:10.1016/S0149-2918(98)80127-6]
16. Bemanian MH, Farhoudi A, Pourpak Z, Gharagozlou M, Movahedi M, Nabavi M, et al. Systemic and local reactions of bee venom immunotherapy in Iran. Iranian Journal of Allergy, Asthma and Immunology (IJAAI) 2007;6:203-6.
17. Borrelli F, Maffia P, Pinto L, Ianaro A, Russo A, Capasso F, et al. Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia 2002;73:S53-63. [DOI:10.1016/S0367-326X(02)00191-0]
18. Farooqui T, Farooqui AA. Beneficial effects of propolis on human health and neurological diseases. Front Biosci (Elite Ed) 2012;4:779-93. [DOI:10.2741/e418]
19. Lahouel M, B.K, Kebsa W, Alyane M. Polyphenolic fractions of Algerian propolis reverses doxorubicin induced acuterenal oxidative stress. African J Pharm Pharmacology (AJPP). 2010; 4: 712-720.
20. Lindner MD, Hogan JB, Hodges DB Jr, Orie AF, Chen P, Corsa JA, Leet JE, Gillman KW, Rose GM, Jones KM, Gribkoff VK. Donepezil primarily attenuates scopolamine-induced deficits in psychomotor function, with moderate effects on simple conditioning and attention, and small effects on working memory and spatial mapping. Psychopharmacology (Berl) 2006;188:629-40. [DOI:10.1007/s00213-006-0556-3]
21. Cardiff RD, Miller CH, Munn RJ. Manual hematoxylin and eosin staining of mouse tissue sections. Cold Spring Harb Protoc 2014; 2: 655-8. [DOI:10.1101/pdb.prot073411]
22. Ayikobua ET, Semuyaba I, Eze DE, Kalange M, Nansunga M, Okpanachi AO, et al. Combined Donepezil and Ethanolic Extract of Propolis Improved Memory Better Than Donepezil and Propolis Monotherapy in Wild Type Drosophila melanogaster. Evidence-Based Complementary and Alternative Medicine (eCAM). 2018;2018. [DOI:10.1155/2018/3717328]
23. Nanaware S, Shelar M, Sinnathambi A, Mahadik KR, Lohidasan S. Neuroprotective effect of Indian propolis in β-amyloid induced memory deficit: impact on behavioral and biochemical parameters in rats. Biomed Pharmacother 2017;93:543-53. [DOI:10.1016/j.biopha.2017.06.072]
24. van Gijsel-Bonnello M, Baranger K, Benech P, Rivera S, Khrestchatisky M, de Reggi M, et al. Metabolic changes and inflammation in cultured astrocytes from the 5xFAD mouse model of Alzheimer's disease: alleviation by pantethine. PloS One 2017;12:e0175369. [DOI:10.1371/journal.pone.0175369]
Send email to the article author

Add your comments about this article
Your username or Email:


XML   Persian Abstract   Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Saeidi M, Rouhollah F. Role of Propolis as a pharmaceutical candidate on interleukin-1β proinflammatory cytokine expression in Alzheimer's rat. MEDICAL SCIENCES. 2021; 31 (4) :388-396
URL: http://tmuj.iautmu.ac.ir/article-1-1828-en.html

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 31, Issue 4 (WINTER 2021) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.05 seconds with 30 queries by YEKTAWEB 4419