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Design and preparation of Chitosan/Alginate nanoparticles containing BSA and In-vitro release study
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Nazli Kashani Javid1   , Nasser Mohammadpour  2, Sepideh Arbabi Bidgoli3 |
1- Department of Medical Nanotechnology, Faculty of Advanced Sciences and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Razi Vaccine and Sera Research Institute, Agricultural Research, Education and Extension Organization, Karaj, Iran , nasser_mohammadpour@yahoo.com
3- Department of Toxicology–Pharmacology, Faculty of Pharmacy, Pharmaceutical Science Branch, Islamic Azad University, Tehran, Iran |
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Abstract: (116 Views) |
Background: Nanoparticles have received more and more attention in the vaccine and drug delivery systems field due to their specific properties. In particular, alginate and chitosan have been known as the most promising nano-carries. The present study aimed to develop alginate/chitosan nanoparticles as a carrier of BSA to Study In-vitro release condition.
Materials and methods: The nanoparticles were produced by the ionic gelation method in mild conditions and the aqueous environment, which leads to the production of particles with favorable size, shape, high entrapment efficiency, and controlled release characteristics.
Results: The synthesized nanoparticles of 60 nm in diameter and -32mv in zeta potential were considerably unparalleled and characterized by SEM, FTIR, and DSC. Nanoparticles resembled a spherical shell, smooth morphology, and homogeneous structure shown by SEM images. FTIR and DSC analyses confirmed conformational changes. Entrapment efficiency and loading capacity were 92% and 74%, respectively, via indirect method. The In-vitro BSA release profile and mechanism of BSA release from the nanoparticles were studied under simulated physiological conditions for various incubation periods. An initial burst effect revealed the release profile, followed by a continuous and controlled release phase.
Conclusion: Our results indicated the prepared nanoparticles could be appropriate for the convenient delivery of the desired drugs.
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| Keywords: Nanoparticles, Chitosan, Alginate, BSA, Release. |
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Full-Text [PDF 1059 kb]
(70 Downloads)
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Semi-pilot: Experimental |
Subject:
Pharmacology
Received: 2023/12/5 | Accepted: 2024/02/27 | Published: 2024/10/1
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Kashani Javid N, Mohammadpour N, Arbabi Bidgoli S. Design and preparation of Chitosan/Alginate nanoparticles containing BSA and In-vitro release study. MEDICAL SCIENCES 2024; 34 (3) :227-236 URL: http://tmuj.iautmu.ac.ir/article-1-2197-en.html
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