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Evaluation of the effect of trans-cinnamic acid on oxidative damage, cholinergic activity of the forebrain, and neuronal density of the medial frontal cortex of the fetus in preeclampsia model rats
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Seyede Fatemeh Hoseiniyan1    , Mohammad Amin Edalatmanesh2 |
1- Msc in Cell and Developmental Biology, Department of Biology, Shi C., Islamic Azad University, Shiraz, Iran
2- Associate Professor of Physiology, Department of Biology, Shi C., Islamic Azad University, Shiraz, Iran , amin.edalatmanesh@gmail.com |
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Abstract: (245 Views) |
Background: Preeclampsia (PE) is a disorder associated with prenatal brain damage. This study investigated the effect of cinnamic acid (CIN) on the parameters of oxidative stress, oxidative DNA damage and cholinesterase (AChE) enzyme activity in the forebrain and the cell structure of the fetal medial frontal cortex (mFC) of l-NAME-induced PE rat model.
Materials and methods: 25 pregnant rats were randomly divided into 5 groups: control group (no treatment), PE+NS group (intraperitoneal administration of 250 mg l-NAME from embryonic day (ED) 15 to ED20 and gavage normal saline), PE+CIN25, PE+CIN50 and PE+CIN100 groups (gavage of cinnamic acid with doses of 25, 50 and 100 mg/kg, after l-NAME injection). On the ED21, with cesarean section of live fetuses, the fetal brain was dissected. AChE, catalase (CAT), superoxide dismutase (SOD), 8-hydroxydeoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels in the forebrain and cell density in the mFC were measured.
Results: A significant decrease in CAT and SOD, and a significant increase in MDA, 6-OHdG and AChE in the forebrain along with a decrease in neuronal density in the mFC of the PE+NS compared to the control were seen (p<0.05). While in the CIN-treated groups, they showed a significant increase in CAT and SOD, a significant decrease in MDA, 6-OHdG, and AChE in the forebrain and an increase in mFC neuronal density compared to the PE+NS (p<0.05).
Conclusion: It seems that CIN with antioxidant effects in the fetal forebrain improves brain function and reduces cell damage in the middle frontal cortex of PE model fetuses.
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| Keywords: Preeclampsia, Cinnamic acid, Antioxidant, Frontal cortex, Fetus. |
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Full-Text [PDF 1357 kb]
(80 Downloads)
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Semi-pilot: Experimental |
Subject:
Animal Biology
Received: 2024/08/27 | Accepted: 2024/11/12 | Published: 2025/05/31
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1. Ma'ayeh M, Costantine MM. Prevention of preeclampsia. Semin Fetal Neonatal Med 2020;25:101123. [ DOI:10.1016/j.siny.2020.101123] 2. Jung E, Romero R, Yeo L, Gomez-Lopez N, Chaemsaithong P, Jaovisidha A, Gotsch F, Erez O. The etiology of preeclampsia. Am J Obstet Gynecol 2022;226:S844-866. [ DOI:10.1016/j.ajog.2021.11.1356] 3. Ives CW, Sinkey R, Rajapreyar I, Tita ATN, Oparil S. Preeclampsia-Pathophysiology and Clinical Presentations: JACC State-of-the-Art Review. J Am Coll Cardiol 2020;76:1690-702. [ DOI:10.1016/j.jacc.2020.08.014] 4. Chaemsaithong P, Sahota DS, Poon LC. First trimester preeclampsia screening and prediction. Am J Obstet Gynecol 2022;226:S1071-97.e2. [ DOI:10.1016/j.ajog.2020.07.020] 5. Dimitriadis E, Rolnik DL, Zhou W, Estrada-Gutierrez G, Koga K, Francisco RPV, Whitehead C, Hyett J, da Silva Costa F, Nicolaides K, Menkhorst E. Pre-eclampsia. Nat Rev Dis Primers 2023;9:8. [ DOI:10.1038/s41572-023-00417-6] 6. Melchiorre K, Giorgione V, Thilaganathan B. The placenta and preeclampsia: villain or victim? Am J Obstet Gynecol 2022;226:S954-62. [ DOI:10.1016/j.ajog.2020.10.024] 7. Jena MK, Sharma NR, Petitt M, Maulik D, Nayak NR. Pathogenesis of Preeclampsia and Therapeutic Approaches Targeting the Placenta. Biomolecules 2020;10:953. [ DOI:10.3390/biom10060953] 8. Bokslag A, van Weissenbruch M, Mol BW, de Groot CJ. Preeclampsia; short and long-term consequences for mother and neonate. Early Hum Dev 2016;102:47-50. [ DOI:10.1016/j.earlhumdev.2016.09.007] 9. Joo EH, Kim YR, Kim N, Jung JE, Han SH, Cho HY. Effect of Endogenic and Exogenic Oxidative Stress Triggers on Adverse Pregnancy Outcomes: Preeclampsia, Fetal Growth Restriction, Gestational Diabetes Mellitus and Preterm Birth. Int J Mol Sci 2021;22:10122. [ DOI:10.3390/ijms221810122] 10. Uzunov AV, Secara DC, Mehedințu C, Cîrstoiu MM. Preeclampsia and neonatal outcomes in adolescent and adult patients. J Med Life 2022;15:1488-92. [ DOI:10.25122/jml-2022-0264] 11. Liu X, Liu H, Gu N, Pei J, Lin X, Zhao W. Preeclampsia promotes autism in offspring via maternal inflammation and fetal NFκB signaling. Life Sci Alliance 2023;6:e202301957. [ DOI:10.26508/lsa.202301957] 12. Postma IR, Bouma A, de Groot JC, Aukes AM, Aarnoudse JG, Zeeman GG. Cerebral white matter lesions, subjective cognitive failures, and objective neurocognitive functioning: A follow-up study in women after hypertensive disorders of pregnancy. J Clin Exp Neuropsychol 2016;38:585-98. [ DOI:10.1080/13803395.2016.1143453] 13. Bakrania BA, George EM, Granger JP. Animal models of preeclampsia: investigating pathophysiology and therapeutic targets. Am J Obstet Gynecol 2022;226:S973-87. [ DOI:10.1016/j.ajog.2020.10.025] 14. Gatford KL, Andraweera PH, Roberts CT, Care AS. Animal Models of Preeclampsia: Causes, Consequences, and Interventions. Hypertension 2020;75:1363-81. [ DOI:10.1161/HYPERTENSIONAHA.119.14598] 15. Stefanovic V, Andersson S, Vento M. Oxidative stress - Related spontaneous preterm delivery challenges in causality determination, prevention and novel strategies in reduction of the sequelae. Free Radic Biol Med 2019;142:52-60. [ DOI:10.1016/j.freeradbiomed.2019.06.008] 16. Sebastiani G, Navarro-Tapia E, Almeida-Toledano L, Serra-Delgado M, Paltrinieri AL, García-Algar Ó, Andreu-Fernández V. Effects of Antioxidant Intake on Fetal Development and Maternal/Neonatal Health during Pregnancy. Antioxidants (Basel) 2022;11:648. [ DOI:10.3390/antiox11040648] 17. Drakontaeidi A, Pontiki E. Multi-Target-Directed Cinnamic Acid Hybrids Targeting Alzheimer's Disease. Int J Mol Sci 2024;25:582. [ DOI:10.3390/ijms25010582] 18. Hemmati AA, Alboghobeish S, Ahangarpour A. Effects of cinnamic acid on memory deficits and brain oxidative stress in streptozotocin-induced diabetic mice. Korean J Physiol Pharmacol 2018;22:257-67. [ DOI:10.4196/kjpp.2018.22.3.257] 19. Safarpour M, Edalatmanesh MA, Hosseini SE. The effect of cinnamic acid on fetal hippocampus in pregnant rats. Comp Clin Pathol 2020; 29: 945-54. [ DOI:10.1007/s00580-020-03118-8] 20. Raghebi R, Mohammadi Safari Kuchi S, Karimi M, Edalatmanesh M A. The effect of Gallic acid on prenatal entorhinal cortex and CA1/CA3 hippocampal areas in trimethyltin intoxication rat. Medical Sciences 2022; 32:293-302. [In Persian] [ DOI:10.52547/iau.32.3.293] 21. Nakamura N, Ushida T, Onoda A, Ueda K, Miura R, Suzuki T, Katsuki S, Mizutani H, Yoshida K, Tano S, Iitani Y, Imai K, Hayakawa M, Kajiyama H, Sato Y, Kotani T. Altered offspring neurodevelopment in an L-NAME-induced preeclampsia rat model. Front Pediatr 2023;11:1168173. [ DOI:10.3389/fped.2023.1168173] 22. Mokhtarkia S, Edalatmanesh M A. Effect of Trans-cinnamic Acid on Cognitive Deficit, Cell Density of CA1/CA3 Regions, and Cholinergic Activity of Hippocampus in Trimethylettin Model of Alzheimer's Disease. J. Ilam Uni Med Sci 2023;31:1-12. [In Persian] 23. Nemati S, Edalatmanesh M A, Forouzanfar M. The effect of Naringin on hippocampal cell damage and the antioxidant defense system of the fetal forebrain in an animal model of uteroplacental insufficiency. Medical Sciences 2023; 33:122-32. [In Persian] 24. Chiarello DI, Abad C, Rojas D, Toledo F, Vázquez CM, Mate A, Sobrevia L, Marín R. Oxidative stress: Normal pregnancy versus preeclampsia. Biochim Biophys Acta Mol Basis Dis 2020;1866:165354. [ DOI:10.1016/j.bbadis.2018.12.005] 25. Nirupama R, Divyashree S, Janhavi P, Muthukumar SP, Ravindra PV. Preeclampsia: Pathophysiology and management. J Gynecol Obstet Hum Reprod 2021;50:101975. [ DOI:10.1016/j.jogoh.2020.101975] 26. Cortés-Albornoz MC, García-Guáqueta DP, Velez-van-Meerbeke A, Talero-Gutiérrez C. Maternal Nutrition and Neurodevelopment: A Scoping Review. Nutrients 2021;13:3530. [ DOI:10.3390/nu13103530] 27. Liu X, Zhao W, Liu H, Kang Y, Ye C, Gu W, Hu R, Li X. Developmental and Functional Brain Impairment in Offspring from Preeclampsia-Like Rats. Mol Neurobiol 2016;53:1009-19. [ DOI:10.1007/s12035-014-9060-7] 28. Hofsink N, Dijkstra DJ, Stojanovska V, Scherjon SA, Plösch T. Preeclampsia-induced alterations in brain and liver gene expression and DNA methylation patterns in fetal mice. J Dev Orig Health Dis 2023;14:146-51. [ DOI:10.1017/S2040174422000344] 29. Check J, Shuster C, Hofheimer J, Camerota M, Dansereau LM, Smith LM, Carter BS, DellaGrotta SA, Helderman J, Kilbride H, Loncar CM, McGowan E, Neal CR, O'Shea TM, Pastyrnak SL, Sheinkopf SJ, Lester BM. Preeclampsia, Fetal Growth Restriction, and 24-Month Neurodevelopment in Very Preterm Infants. JAMA Netw Open 2024;7:e2420382. [ DOI:10.1001/jamanetworkopen.2024.20382] 30. Johnson AC, Tremble SM, Cipolla MJ. Experimental Preeclampsia Causes Long-Lasting Hippocampal Vascular Dysfunction and Memory Impairment. Front Physiol 2022;13:889918. [ DOI:10.3389/fphys.2022.889918] 31. Chen ZR, Huang JB, Yang SL, Hong FF. Role of Cholinergic Signaling in Alzheimer's Disease. Molecules 2022;27:1816. [ DOI:10.3390/molecules27061816] 32. Cox MA, Bassi C, Saunders ME, Nechanitzky R, Morgado-Palacin I, Zheng C, Mak TW. Beyond neurotransmission: acetylcholine in immunity and inflammation. J Intern Med 2020;287:120-33. [ DOI:10.1111/joim.13006] 33. Antartani R, Ashok K. Effect of lycopene in prevention of preeclampsia in high risk pregnant women. J Turk Ger Gynecol Assoc 2011;12:35-38. [ DOI:10.5152/jtgga.2011.08] 34. Rahnemaei FA, Fashami MA, Abdi F, Abbasi M. Factors effective in the prevention of Preeclampsia:A systematic review. Taiwan J Obstet Gynecol 2020;59:173-82. [ DOI:10.1016/j.tjog.2020.01.002] 35. Stupakova EG, Lazareva GA, Gureev VV, Dolzhikova IN, Zhilinkova LA, Gureeva AV. L-NAME-induced preeclampsia: correction of functional disorders of the hemostasis system with resveratrol and nicorandil. Res Result Pharmacol 2019;5:1-12. [ DOI:10.3897/rrpharmacology.5.35316] 36. Elkharsawy H, Eldomany RA, Mira A, Soliman AF, Amir M, El-Sharkawy S. New neuroprotective derivatives of cinnamic acid by biotransformation. Food Funct 2024;15:4323-37. [ DOI:10.1039/D3FO04802K] 37. Pozdnyakov DI. 4-Hydroxy-3,5-di-tret-butyl cinnamic acid restores the activity of the hippocampal mitochondria in rats under permanent focal cerebral ischemia. Iran J Basic Med Sci 2021;24:1590-601. [ DOI:10.22541/au.161058198.82817521/v1] 38. Ren Z, Zhang R, Li Y, Li Y, Yang Z, Yang H. Ferulic acid exerts neuroprotective effects against cerebral ischemia/reperfusion-induced injury via antioxidant and anti-apoptotic mechanisms in vitro and in vivo. Int J Mol Med 2017;40:1444-56. [ DOI:10.3892/ijmm.2017.3127] |
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Hoseiniyan S F, Edalatmanesh M A. Evaluation of the effect of trans-cinnamic acid on oxidative damage, cholinergic activity of the forebrain, and neuronal density of the medial frontal cortex of the fetus in preeclampsia model rats. MEDICAL SCIENCES 2025; 35 (2) :145-155 URL: http://tmuj.iautmu.ac.ir/article-1-2298-en.html
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