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:: Volume 20, Issue 4 (winter 2011) ::
MEDICAL SCIENCES 2011, 20(4): 246-251 Back to browse issues page
Production of collagen type I and its using in medical practice
Mohammad Karim Rahimi 1, Ramin Khajavi2 , Mohammad Amin Mahdavi Hezaveh3 , Elnaz Ebrahimi Hour4
1- Department of Microbiology, Tehran Medical Branch, Islamic Azad University, Tehran, Iran , mohammadkrahimi@yahoo.com
2- Postgraduate center, Tehran South Branch, Islamic Azad University, Tehran, Iran
3- Tehran south Branch, Islamic Azad University, Tehran, Iran
4- Tehran Medical Branch, Islamic Azad University, Tehran, Iran
Abstract:   (33596 Views)
Background: Collagen is a protein with specific importance in pharmacy and medicine. The aim of this study was extraction of collagen type I by optimum using of Iranian fishes of Persian Gulf and also debris of Shilat industry.
Materials and methods: Extraction of collagen type I from the proper specimens was performed by two methods of acidic and alkaline. In acidic method, first, the lipids separated, then, by using HCL 5%, calcium elements were extracted. The pH of medium neutralized and hydrolysis was performed in high stream water vapor. The material have been filtered and in the vicinity of heat in vacuum state collagen concentrated. Then the sheets of collagen, grinding was done. In the alkaline method, the specimens were treated by sodium hydroxid 4% for 3 weeks at room temperature.
Results: The rate of separated collagen from fish hasn’t significant difference between alkaline and acidic methods. For example, mean rate of 20.81% for collagen extraction from skin of fishes in alkaline method and mean of 19.76% in acidic method was detected. The collagen specimens extracted by these methods have equal or higher quality in comparison with specimens extracted from other sources.
Conclusion: According to costless of primary materials, such as debris of fishes, in the production of collagen in Iran and import of collagen is expensive, domestic production is saving money.
Keywords: Skin and debris of fish, Extraction method, Collagen type I, Medical practices
Full-Text [PDF 560 kb]   (9075 Downloads)    
Semi-pilot: Basic | Subject: Labratory Sciences
Received: 2011/02/12 | Accepted: 2013/05/28 | Published: 2013/05/28
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Rahimi M K, Khajavi R, Mahdavi Hezaveh M A, Ebrahimi Hour E. Production of collagen type I and its using in medical practice. MEDICAL SCIENCES 2011; 20 (4) :246-251
URL: http://tmuj.iautmu.ac.ir/article-1-376-en.html

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Volume 20, Issue 4 (winter 2011) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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