Background: There are several studies that have identified relationship between VDR gene polymorphisms and colorectal (CRC) or other kinds of cancers, such as breast and prostate cancers. The aim of our study was to evaluate the association of VDR gene polymorphisms, BsmI and FokI, with colorectal cancer risk among Iranian patients.

Materials and methods: In this case-control study, 110 DNA samples from Iranian CRC patients and 110 samples from healthy Iranian people. Genotyping of BsmI and FokI polymorphisms were performed by PCR-RFLP method. To confirm the RFLP results, 5% of samples were sequenced with direct sequencing method.

Results: The frequency of the VDR gene polymorphisms at BsmI and FokI restriction sites in CRC patients and healthy controls was almost similar. Allele distribution in patients and controls was same. There was no statistically significant difference in genotype or allele frequency between CRC patients and control group.

Conclusion: VDR FokI and BsmI genotypes are not associated with increased risk of colorectal cancer in Iranian patients. However, these data remain to be confirmed by studies with larger sample size in Iran