[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
Main Menu
Home::
Journal Information::
Articles archive::
For Authors::
For Reviewers::
Registration::
Contact us::
Site Facilities::
Webmail::
::
Search in website

Advanced Search
..
Receive site information
Enter your Email in the following box to receive the site news and information.
..
:: Volume 24, Issue 1 (Spring 2014) ::
MEDICAL SCIENCES 2014, 24(1): 1-7 Back to browse issues page
The effect of new born NMRI mouse heart extract on differentiation of NMRI mouse embryonic cord stem cells
Kazem Parivar1 , , Maryam Momayez sefat2 , Nasim Hayati Roodbari3 , Maryam Bananaj4
1- Department of Animal Biology, School of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
2- Department of Animal Biology, School of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran , maryam_ms60@ yahoo.com
3- Department of Animal Biology, School of Basic Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran
4- Department of Animal Biology, School of Biological Sciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
Abstract:   (10063 Views)
Background: Stem cells are cells with high regeneration ability and potential to develop into many different cell types in the body. Stem cells are able to differentiate into specialized cell of different tissues in vitro and are used for cell therapy. In the present study, the ability of umbilical cord mesenchymal stem cells to differentiate in to cardiomyocytes like cells in vitro was investigated.
Materials and methods: In this experimental study, the effect of heart extract on differentiation of mesenchimal stem cells was assessed in vitro. First, MSCs were isolated from mouse NMRI umbilical cord on E16-18 and cultured in plate under a humidified atmosphere of 5% CO2 in air at 36.5◦C. The day 7–10 of culture, these cells received 40 λ heart extract from 2 weeks old mice every 4 days. After induction for 21 days, mesenchymal cells started to differentiate into cardiomyocytes like cells. Immunocytochemistry methods were used to detect the expression of alpha smooth muscle actin protein. Results: Morphological changes and immunocytochemistry studies showed the expression of alpha smooth muscle actin and indicated that the MSCs could have the potential to differentiate into cardiomyocytes like cells in vitro.
Conclusion: These results indicate that umbilical cord mesenchimal stem cells can differentiate into cardiomyocytes like cells in vitro by some growth factors existing in the heart extract.
Keywords: Umbilical cord, Mesenchymal stem cells, Heart extract, Differentiation, Cardiomyocytes like cells.
Keywords: Umbilical cord, Mesenchymal stem cells, Heart extract, Differentiation, Cardiomyocytes like cells.
Full-Text [PDF 414 kb]   (2579 Downloads)    
Semi-pilot: Experimental | Subject: Animal Biology
Received: 2014/03/9 | Accepted: 2014/03/9 | Published: 2014/03/9
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

parivar K, Momayez sefat , M, Hayati Roodbari N, Bananaj M. The effect of new born NMRI mouse heart extract on differentiation of NMRI mouse embryonic cord stem cells . MEDICAL SCIENCES 2014; 24 (1) :1-7
URL: http://tmuj.iautmu.ac.ir/article-1-764-en.html


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 24, Issue 1 (Spring 2014) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
Persian site map - English site map - Created in 0.07 seconds with 37 queries by YEKTAWEB 4710