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Isolation and assessment of the pigments from the psychrotrophic bacteria with the aim of application in the food industries
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Hadiseh Pazoki1   , Parastoo Pourali 2 |
1- Microbiology, Department of Biology, Neyshabur Branch, Islamic Azad University, Neyshabur, Iran
2- Department of basic science, Faculty of medical science, Shahrood branch, Islamic Azad University, Shahrood, Iran , Parastoo_pourali@yahoo.com |
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Abstract: (5514 Views) |
| Background: Nowadays, the use of the organic pigments produced by the microorganisms according to their nature and safety is increasing. These pigments are various in their nature and have different properties. The aim of the recent research was to extract the new bacterial pigments that are produced in the cold environments.
Materials and methods: Ten soil samples from different areas of Torghabeh city were collected and cultivated. Ten days after, 14 pigmented bacterial colonies were selected then the isolates were identified using the biochemical tests and polymerase chain reaction (PCR). Using the methanol and glass powder, bacterial pigments were extracted and in order to purification TLC was performed. Pigments of two strains, A8 and A13, which had a suitable purity, were used for cell toxicity using three cell lines HepG2, human embryonic fibroblasts, and gastric cancer.
Results: Among 14 pigmented bacterial strains, the strain A8 that was Aeromonas hydrophyla, could prevent the growth of the cells on different cancerous cell line. Also, the pigment that was named A13, had no cytotoxic effects on all the used cell types and can be used as a natural coloring agent in the food industry.
Conclusion: As the results indicate based on the biological properties of the extracted bacterial pigments, they can be used in different areas such as medicine and technology.
Keywords: Psychrotrophic bacteria, Pigments, Food industry, Anti cancer effect. |
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| Keywords: Psychrotrophic bacteria, Pigments, Food industry, Anti cancer effect. |
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Full-Text [PDF 1339 kb]
(2131 Downloads)
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Semi-pilot: Survey/Cross Sectional/Descriptive |
Subject:
Molecular Biology
Received: 2016/10/15 | Accepted: 2017/02/1 | Published: 2017/06/19
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