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:: Volume 30, Issue 2 (summer 2020) ::
MEDICAL SCIENCES 2020, 30(2): 120-127 Back to browse issues page
The effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways gastrocnemius muscle of type 2 diabetic rats
Golnaz Faezi1 , Mohammad Sherafati Moghadam2 , Saeedeh Shadmehri 3, Mohammad Fathalipour4
1- Faculty Member of Kharazmi University, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran
2- Department of Pure and Basic Science, Hashtgerd Branch, Islamic Azad University, Alborz, Iran
3- Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, I. R. Iran. , saeedehsh61@gmail.com
4- Assistant Professor, Department of Pharmacology, Faculty of Pharmacy, Hormozgan University of Medical Sciences, Bandar Abbas, Iran
Abstract:   (3263 Views)
Background: The pathway of mTORC1 is one of the most important pathway in protein synthesis, and type 2 diabetes and insulin resistance can lead to inhibit this pathway. The aim of this study was to investigate the effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways in gastrocnemius muscle in type 2 diabetic rats.
Materials and methods: In this experimental study, 16 Sprague-Dawley male rats (with mean weight of 250 ± 20 gr) were selected and after induction of diabetes by streptozotocin and nicotinamide, the rats were randomly assigned into two groups, including diabetic HIIT training and diabetic control. The experimental group performed 4 days a week the exercise training for 4 weeks, while the control group did not have any training program. Independent T-test was used to analyze the data.
Results: A significant change was not observed in the total content of AKT1 proteins (p<0.31), P70S6K1 (p<0.69) and 4EBP1 (p<0.84) in the HIIT training group compared to the control group, but the total protein content of mTOR (p<0.02) and the form of phosphorylation of AKT1 (p<0.03), mTOR (p<0.03), P70S6K1 (p<0.02) and 4EBP1 (p<0.009) proteins showed significant increase in training group compared to the control group.
Conclusion: The HIIT training can probably activate the pathway of AKT1/mTOR/P70S6K1 and AKT1/mTOR/4EBP1 in the mTORC1 pathway; therefore, the HIIT training can lead to protein synthesis or muscle hypertrophy through this pathway in the gastrocnemius muscle of the trained rats
Keywords: Gastrocnemius muscle, High-intensity interval training, mTORC1 pathway, Type 2 diabetes
Full-Text [PDF 418 kb]   (1627 Downloads)    
Semi-pilot: Experimental | Subject: Physiology
Received: 2019/04/27 | Accepted: 2019/07/23 | Published: 2020/06/28
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Faezi G, Sherafati Moghadam M, Shadmehri S, Fathalipour M. The effect of 4 weeks high-intensity interval training (HIIT) on the content of downstream and upstream mTORC1 pathways gastrocnemius muscle of type 2 diabetic rats. MEDICAL SCIENCES 2020; 30 (2) :120-127
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Volume 30, Issue 2 (summer 2020) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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