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:: Volume 30, Issue 4 ( winter 2020) ::
MEDICAL SCIENCES 2020, 30(4): 425-431 Back to browse issues page
Method validation and determination of caffeine in drinks in the Iranian market by the HPLC
Hamide Sharifi Darani1, Parisa Ziarati2, Mahboubeh Taherkhani3, Zahra Mousavi 4
1- PharmD Student, Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University (IAUPS), Tehran, Iran
2- Nutrition and Food Sciences Research Center, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , Assistant Professor, Medicinal Chemistry Department, Pharmacy Faculty, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
3- Assistant Professor, Department of Chemistry, College of Sciences, Takestan Branch, Islamic Azad University, Takestan, Iran
4- Associate Professor, Department of Pharmacology and Toxicology, Faculty of Pharmacy and Pharmaceutical Sciences, Tehran Medical Sciences, Islamic Azad University (IAUPS), Tehran, Iran , mosavi50@yahoo.com
Abstract:   (373 Views)
Background: Caffeinated energy drinks are common drinks among athletes and general population that have benefits such as providing quick energy, reducing fatigue and creating alertness, but can lead to serious complications in patients, especially those who have cancer and mental, cardiovascular, or renal diseases. The aim of this study was to validate the quantitative analysis and determine caffeine in 10 caffeine-containing drink samples from Tehran markets using high efficiency liquid chromatography (HPLC).
Materials and methods: In this study, first the HPLC method was validated using the sample standard. Then the amount of caffeine in 10 samples of drinks was evaluated.
Results: The calibration curve of caffeine was linear in the range of 2-200 ppm and its correlation coefficient was 0.997. The limits of detection and quantification (LOQ and LOD) were 44.65 and 13.39 mg/mL respectively. After validating the method, 10 drink samples collected from Tehran were tested for caffeine content. Comparing the results with international standards showed that the caffeine value of these beverages was lower than the standard.
Conclusion: This method has the accuracy and efficiency in measuring the caffeine of beverages. Comparing with international standards, the amount of caffeine in the beverages is lower than the standard. Due to the increased consumption of these drinks in the country, measuring caffeine at different times is essential.
Keywords: Caffeinated drinks, High efficiency liquid chromatography, Method validation, Caffeine value
Full-Text [PDF 450 kb]   (202 Downloads)    
Semi-pilot: Experimental | Subject: Pharmacology
Received: 2019/10/14 | Accepted: 2020/01/13 | Published: 2020/12/20
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Sharifi Darani H, Ziarati P, Taherkhani M, Mousavi Z. Method validation and determination of caffeine in drinks in the Iranian market by the HPLC. MEDICAL SCIENCES. 2020; 30 (4) :425-431
URL: http://tmuj.iautmu.ac.ir/article-1-1849-en.html


Volume 30, Issue 4 ( winter 2020) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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