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:: Volume 31, Issue 4 (WINTER 2021) ::
MEDICAL SCIENCES 2021, 31(4): 406-412 Back to browse issues page
Determination of hemolysine genes frequency in antibiotic-resistant Staphylococcus aureus isolated from wound and urine samples of patients
Mina Aghsafi1 , Zahra Tahmasebi Fard 2
1- MSc of Microbial Biotechnology, Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran
2- Associated Professor of Cellular and Molecular Biology, Department of Biology, Roudehen Branch, Islamic Azad University, Roudehen, Iran , ztahmasebifard@yahoo.com
Abstract:   (1791 Views)
Background: Staphylococcus aureus is a pathogenic bacterium that can target host cell membranes with virulence factors such as toxins and peptides. This study was evaluated the frequency of alpha, beta, and delta hemolysine genes in antibiotic-resistant Staphylococcus aureus isolated from patients' urine and wound samples.
Materials and methods: This cross-sectional study was performed on 100 antibiotic-resistant Staphylococcus aureus (tetracycline, penicillin, gentamicin, co-trimoxazole, tobramycin, and ciprofloxacin) isolated from wound and urine samples of patients who referred to medical diagnostic laboratories. Then, their antibiotic susceptibility was determined by the disk diffusion method based on CLSI instruction. After examining the MIC and MBC of the samples, a single colony culture was performed for the samples and their DNA was extracted by using a commercial kit. By designing specific primers, hemolysine alpha, beta, and delta genes were amplified in the samples. The results were analyzed by statistical software.
Results: In isolated Staphylococcus aureus, the highest antibiotic resistance was related to co-trimoxazole with 63% and the highest sensitivity to penicillin with 53%. There was a statistically significant relationship between resistance to tetracycline and co-trimoxazole antibiotics and age, and also ciprofloxacin showed greater resistance in women than men. The frequency of hemolysine genes was 91% for HLA, 96% for HLB, and 99% for HLD.
Conclusion: Penicillin antibiotic with 53% sensitivity seems to be a better candidate than other antibiotics and the high abundance of hemolysine genes should be considered as an important concern in the medical community.
Keywords: Alpha hemolysine, Beta hemolysine, Delta hemolysine, Staphylococcus aureus.
Full-Text [PDF 418 kb]   (1470 Downloads)    
Semi-pilot: case-control | Subject: Microbiology
Received: 2021/07/10 | Accepted: 2021/09/15
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Aghsafi M, Tahmasebi Fard Z. Determination of hemolysine genes frequency in antibiotic-resistant Staphylococcus aureus isolated from wound and urine samples of patients. MEDICAL SCIENCES 2021; 31 (4) :406-412
URL: http://tmuj.iautmu.ac.ir/article-1-1897-en.html
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Volume 31, Issue 4 (WINTER 2021) Back to browse issues page
فصلنامه علوم پزشکی دانشگاه آزاد اسلامی واحد پزشکی تهران Medical Science Journal of Islamic Azad Univesity - Tehran Medical Branch
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