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Effects of Diamond nanoparticles on mitochondrial apoptosis pathway in rats with testicular ischemia / reperfusion.
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Masoumeh Masoumi1   , Mitra Salehi2 , Seyed Abdolhamid Angaji3 , Mehrdad Hashemi 4 |
1- Department of Genetics, faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
2- Department of Microbiology, faculty of biosciences, Islamic Azad University, North Tehran Branch, Tehran, Iran
3- Department of Cell and Molecular Biology, Faculty of biosciences, ,Kharazmi university, Tehran, Iran
4- Department of Genetics, faculty of Advanced science and Technology, Islamic Azad University, Tehran Medical Sciences, Tehran, Iran ----Farhikhtegan Medical Convergence Sciences Research Center, Farhikhtegan Hospital Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , mhashemi@iautmu.ac.ir |
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Abstract: (564 Views) |
Background and Aim: Testicular torsion is a urological emergency that leads to testicular tissue damage and infertility. In recent years, several studies have been conducted on the antioxidant properties of nanoparticles. The aim of this study was to investigate the effects of Diamond nanoparticles on the apoptosis in rats with testicular ischemia / reperfusion.
Methods: 48 adult rats aged 20 weeks and weighing 250 g in the control and ischemia / testicular reperfusion groups were included in the study. Both groups were treated with q10 and nanodiamond at a dose of LD50 for 10 days. Then the testes were removed and the survival and apoptosis of the cells were assessed by MTT and flowcytometry and the expression of BAX and BCL2 genes was measured.
Results: Treatment with nanodiamond in the control group increased the expression of BAX gene and decreased the expression of BCL2 gene, while in the ischemia / reperfusion group it had the opposite effect. Simultaneous use of Q10 and nanodiamond compensated for the adverse effects of nanodiamond (P <0.05).
Conclusion: Nanodiamond at LD50 dose in rats with testicular ischemia / reperfusion had antioxidant effects and reduced apoptosis.
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| Keywords: Diamond nanoparticles, antioxidant, ischemia / reperfusion, apoptosis |
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Received: 2022/08/24 | Accepted: 2021/12/31 | Published: 2021/12/31
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