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Microbial synthesis of protein nanoparticles containing silver with Bifidobacterium breve and evaluation their physiochemical properties
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Kimia Parsi1    , Masoud Javanmardi2 , Sepideh Khaleghi 3 |
1- Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2- Department of Medical Biotechnology, Applied Biophotonics Research Center, Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
3- Department of Biotechnology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran , sp.khaleghi@gmail.com |
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Abstract: (317 Views) |
Background: Biological and microbial synthesis processes of nanoparticles are a safe and useful option compared to physical and chemical methods due to their compatibility with the environment and nature and their cost-effectiveness. Probiotics have beneficial effects in improving the body's immune system and are a suitable alternative in the treatment of bacterial infections. The aim of this study was the synthesis of protein nanoparticles containing silver with the probiotic bacteria Bifidobacterium breve.
Materials and methods: First, probiotic bacteria Bifidobacterium breve were cultured and then silver albumin nanoparticles (Ag-Alb-NPs) were synthesized. X-ray diffraction (XRD), infrared spectroscopy (FTIR), transmission electron microscopy (TEM), scanning electron microscopy (SEM) and dynamic light scattering (DLS) tests to investigate the physical and chemical properties of Ag-Alb-NPs used.
Results: Ag-Alb-NPs had spherical, crystalline, semi-crystalline morphology and structure. They had O-H, N-H and C-H bonds, C=C bonds in aromatic rings and C-N bonds in amine compounds and O-H and C-H bonds in CH2 and CH3 groups, N-H, C-O bonds in the structure of C-O-C and C-OH functional groups. In addition, DLS results of albumin-silver nanoparticles and silver nanoparticles showed that they were 259 and 85.3 nm in size, respectively, and the graph was of single peak type.
Conclusion: In general, it was concluded that the synthesized Ag-Alb-NPs had suitable structural and physicochemical characteristics that can be used for future studies.
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| Keywords: Albumin, Nanoparticles, Silver, Probiotics, Bifidobacterium breve. |
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Full-Text [PDF 1432 kb]
(198 Downloads)
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Semi-pilot: Experimental |
Subject:
Nanobiotechnology
Received: 2023/11/19 | Accepted: 2024/03/9 | Published: 2024/11/30
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Parsi K, Javanmardi M, Khaleghi S. Microbial synthesis of protein nanoparticles containing silver with Bifidobacterium breve and evaluation their physiochemical properties. MEDICAL SCIENCES 2024; 34 (4) :345-356 URL: http://tmuj.iautmu.ac.ir/article-1-2190-en.html
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